Study of the Association of Vitamin D Receptor (VDR) Gene Polymorphism in Iraqi Patients with Inflammatory Bowel Disease

Abstract

Inflammatory bowel disease (IBD) is a chronic inflammatory disorder divided into two types, Ulcerative colitis (UC), and Crohnʹs disease (CD). In total, 80 blood samples were collected for this study, 40 blood samples were collected from IBD and 40 blood samples were collected from healthy individuals as. Genomic DNA extracted from whole blood and (FokI) polymorphism was detected by polymerase chain reaction Restriction fragment length polymorphism (PCR- RFLP). The results of the statistical analysis showed high frequency and percentage in age group (< 30 years) was 16 (40%) with significant (P≤0.05) when compeered with control group. The frequency and percentage of body mass index (BMI) for the patient group was (27.91 ± 0.45). While BMI was (27.31 ± 0.54) for the control group with highly significant association (p<0.01) between Iraqi patients with IBD when compeered with control group.(VDR- FokI) wild allele PCR products (without restriction site) have 265bp (F allele) and when digested and electrophoresis of the digestion products on agarose gel the resulted fragment size of 196bp, 265 bp and 69 bp (f allele).The genotype of FokI gene (TT/FF) was 14 (35%) (P-value=0.0039), TG / Ff 15(37.5%) (p= 0.0253), GG /ff 11(27.5%) (p=0.0039) in patient group which have a highly significant in frequency and percentage, as compared with control group. In conclusion The FokI genes polymorphism was shown to be associated with an increased incidence of Iraqi patients with IBD when compeered with control group. The genetic factors of FokI gene polymorphism may have a role in inflammatory bowel disease.

Study the Relationship between polymorphisms of Vitamin D Receptor (VDR FokI and VDR ApaI) Genes and colorectal cancer in some Iraqi population

“…There are eleven exons in total [20, 21,22] even though many SNPs linked to VDR function have been reported, related studies have largely focused on five frequent SNPs: FokI, Tru9I, BsmI, ApaI, and TaqI, which are usually coinherited as haplotype blocks [23]. Important functional SNPs in the VDR gene are: the FokI (C/T) found at the 5′ end of exon 2 in ch-12 location 47879112 (rs2228570) whereas ApaI G/T in ch-12 at location 47845054 (rs7975232) are found inside intron 8 [24,25,26]. The FokI (C/T allele) polymorphism is an independent marker among many polymorphisms located in the VDR gene.…”