Study of the drop size frequencies in a microbial growth system with an aqueous-organic culture medium: lipase production from Candida rugosa

A comprehensive study of lipase production byYarrowia lipolyticaCECT 1240 (ATCC 18942): from shake flask to continuous bioreactor

“…Some authors have reported the significant influence of agitation speed on lipase production by yeasts, namely Candida rugosa, for which higher enzyme biosynthesis levels were found at low agitation speeds, and therefore large drop sizes in the fatty acid/water emulsion. 6 Similar conclusions were also reached for Y. lipolytica. 7,8 First, an air lift bioreactor was employed, and operated at two different air rates (0.40 and 0.75 vvm) in order to preliminarily assess the potential effect of this variable in the process.…”

Candida rugosa Lipase: A Traditional and Complex Biocatalyst

“…From Table 1 we can also assume that there is not a direct relationship between lipase production and cell growth [11,39,40], in agreement with the conclusions reported by Montesinos et al [41], who showed that the substrate/biomass ratio is the key variable for the induction of lipase (inducer, oleic acid). In addition, Lotti et al [11] have shown that the transport of the protein towards the periplasmic zone may be the limiting secretion step, so that this transport controls the production of CRL.…”

Study of drop and bubble sizes in a simulated mycelial fermentation broth of up to four phases

“…The oils, in addition to being the main or a supplementary carbon source, have been claimed to protect fragile mutants and also to reduce the concentration in the media of some inhibitory metabolites, including the antibiotics themselves, by virtue of their high solubility in the oil. Also, the use of oils has obvious advantages in the production of lipidsoluble materials (e.g., ␤-carotene) and in the production of lipases (Dalmau et al, 1998;Geraats, 1994); furthermore, the use of oils is beneficial in improving the yields of a variety of fermentations, such as those for the production of riboflavin and citric acid (Ratledge, 1977). Another threephase system of interest is found in the biodesulfurization of crude oil (Tsouris et al, 1996) where the biocatalyst is a sulfate-reducing bacteria, suspended in the aqueous phase.…”

“…The size distribution of oil drops in a three-phase fermentation was described by Dalmau et al (1998) in batch cultures of Candida rugosa in the presence of oleic acid at 2 g L −1 [i.e., ⌽ in Eq. (2) equal to ∼0.002] aerated at 0.6 vvm.…”