Study of the enantioseparation capability of chiral dual system based on chondroitin sulfate C in CE

Abstract

It has been reported that chiral dual system is able to improve the enantioseparation of enantiomers in many cases. Currently, the dual systems involved in CE chiral separation are mostly dual CDs systems, and the polysaccharides-based chiral dual system was reported in only one paper. To the best of our knowledge, the use of chondroitin sulfate C (CSC)-based dual system for enantiomeric separation has not been reported previously. Herein, four CSC-based chiral dual systems, namely CSC/glycogen, CSC/chondroitin sulfate A (CSA), CSC/hydroxypropyl-β-CD (HP-β-CD), as well as CSC/β-CD (β-CD), were evaluated for the first time for their enantioseparation capability by CE in this paper. During the course of the work, the influences of chiral selector concentration and buffer pH values on enantioseparation in dual systems were systematically investigated. Under the optimized conditions, the dual system consisting of CSC and glycogen exhibited better separations toward nefopam, duloxetine, sulconazole, atenolol, laudanosine, and cetirizine enantiomers compared to the single CSC or glycogen system. The combination of CSC and HP-β-CD improved the separation of amlodipine and chlorphenamine enantiomers. However, no synergistic effect was observed in the CSC/CSA and CSC/β-CD systems.

Synergistic enantioseparation systems with either cyclodextrins or cyclofructans and L‐alanineTertbutyl ester lactate

“…On the other hand, the chiral ionic liquid, which, in most cases, improves both efficiency and resolution significantly, is simple and cheap to synthesize . In addition, the analysis times of NEF , FXD , WAR , and COU were sufficiently lower than the ones reported in the literature.…”

Chiral selectors in CE: Recent development and applications (mid‐2014 to mid‐2016)

“…The combination of these two CSs resulted in greater resolution values (>1.5), while in the case of a single‐CS system, no or partial chiral separations were achieved. In 2015, the polysaccharide chondroitin sulfate C (CSC) was combined with several CSs (chondroitin sulfate A, glycogen, HP‐β‐CD, and β‐CD), and the enantioseparation of different analytes was investigated . The results indicated that, in comparison to the single system, the system CSC/glycogen enhanced the enantioseparations significantly.…”

Study of Different Chiral Columns for the Enantiomeric Separation of Azoles Using Supercritical Fluid Chromatography

“…Considering these differences between pairs of enantiomers, the development of enantioselective analytical methods is an important task. Several analytical techniques have been applied for the enantiomeric separation of antifungal compounds, such as gas chromatography (GC) [4], capillary electrophoresis (CE) [5][6][7][8][9][10][11], high-performance liquid chromatography (HPLC) [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31] and supercritical fluid chromatography (SFC) [14,[32][33][34][35][36][37][38][39]. Commercially or hand-made chiral stationary phases (CSPs), based on different cellulose [12,[14][15][16][19][20][21][22][23][25]…”