Study of the experimental conditions for the lipase production by a newly isolated strain of Pseudomonas aeruginosa for the enantioselective hydrolysis of (±)-methyl trans-3(4-methoxyphenyl) glycidate

Abstract

A lipase producing bacterium has been isolated from the soil to enantiospecifically hydrolyze the (+/-)-methyl trans-3(4-methoxyphenyl) glycidate (MPGM), an intermediate in the synthesis of cardiovascular drug, diltiazem. This hydrolysis provided the desired (-)-MPGM in 44% yield with 99% enantiomeric excess. The organism was identified and confirmed as Pseudomonas aeruginosa by 16S rRNA sequencing. The various physiochemical parameters have been optimized for the maximum production of lipase in shake flask. Beef extract was found to be the best nitrogen source for lipase production. The optimized cultivation conditions were 30 degrees C with an initial medium pH 8 in shake flask. Both inoculum age and inoculum concentration have positive effect on the lipase production and (+/-)-MPGM (3 mM) was found to be the optimal inducer.

Enhanced lipase production from Aeromonas sp. S1 using Sal deoiled seed cake as novel natural substrate for potential application in dairy wastewater treatment

“…Very poor enzyme activities were observed at pH 5.0 (4.7 U mL −1 ), 7.0 (63 U mL −1 ) and 10.0 (39 U mL −1 ), while at pH 9.0 good lipase production of 111 U mL −1 was obtained. Similar pH optima of 8.0 for maximum lipase production have been reported by Immanuel et al 18 and Singh et al 20 for Serratia rubidaea and Pseudomonas aeruginosa , respectively.…”

“…At 25 °C, 171.6 U mL −1 of lipase activity was detected. Singh et al 20 and Kanwar et al 24 also reported optimized temperatures of 30 °C and 34 °C for lipase production by a newly isolated strain of Pseudomonas aeruginosa and Pseudomonas sp. respectively.…”

Chiral resolution of differently substituted racemic acetyl-1-phenyl ethanol using lipase from Bacillus subtilis

“…Maximum lipase production with sucrose was reported among the various carbohydrates sources including glucose, galactose, fructose, xylose and sucrose used as carbon source 12. Beef extract was found to be the best nitrogen source for production of lipase from Pseudomonas aeruginosa at shake flask level 13. Fadiloglu and Erkmen14 reported that the production of lipase from Candida rugosa yielded highest enzyme activity (5.58 U mL −1 ) with yeast extract and proteose‐peptone medium with olive oil whereas the minimum lipase activity (2.81 U mL −1 ) was observed with tryptone and lactose medium.…”