Study of the Properties of the Mixed Water/Salt House Dust Mite Allergen Aiming to Design Its Sublingual Form

Berzhets, V M; Khlgatian, Svetlana V.; Petrova, Stanislava Yu.; Khruleva, V. A.; Efremov, M. A.; Vasilyeva, Anna V.; Petrova, Nina S.; Akutina, V. A.

doi:10.15789/1563-0625-2018-4-597-600

Cited by 3 publications

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“…разработана технология приготовления гранулированной лечебной формы для сублингвального применения. Данная лечебная форма представляет собой набор из восьми последовательных десятичных разведений (Д1-Д8) стандартного экстракта микст-аллергена, нанесенного на сахарную крупку [15].…”

Section: Introductionunclassified

Study of the specific activity of the granulated form of the mixed-allergen from house dust mites Dermatophagoides pteronyssinus and Dermatophagoides farinae for oral immunotherapy

Berzhets¹,

Koreneva²,

Khlgatian³

et al. 2019

Immunopathol Allergol Infectol

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Цель. Изучить свойства гранулированной формы миксталлергена из Dermatophagoides pteronyssinus (Der.p.) и Dermatophagoides farinae (Der.f.). Методы. Для определения наличия аллергенов в экспериментальном препарате был использован микроточечный иммуноблот. Для изучения иммуногенных свойств экспериментального аллергена (ЭА) проводилась иммунизация морских свинок с последующим изучением сывороток крови животных методом ИФА. Результаты. Доказано, что гранулированная форма микст-аллергена из клещей Der.p. и Der.f. обладает аллергенспецифической активностью. ЭА вызывает статистически достоверный прирост специфических к Der.p. и Der.f. IgG антител у иммунизированных морских свинок по сравнению с контрольной группой. Выводы. Полученные результаты свидетельствуют о возможности применения препарата для иммунотерапии. Ключевые слова Гранулированная форма аллергена, клещи домашней пыли, Dermatophagoides pteronyssinus, Dermatophagoides farinaе, сублингвальная иммунотерапия Summary Objective: To study the properties of the granulated form of the mixed-allergen from Dermatophagoides pteronyssinus (Der.p.) and Dermatophagoides farinae (Der.f.). Methods: Immunodot was used for determination of allergens in the experimental preparation. Guinea pigs were immunized to study the immunogenic properties of the experimental allergen (ЕА), this was followed by the study of animal blood serum by ELISA. Results: It has been proved, that the granulated form of the mixed-allergen from Der.p. and Der.f. has an allergen specific activity. The ЕА causes a statistically significant increase of specific IgG antibodies to Der.p. and Der.f. among immunized Guinea pigs compared to the control group. Conclusion: The results indicate possible therapeutic efficacy of the ЕА in sublingual immunotherapy.

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Section: Introductionunclassified

Study of the specific activity of the granulated form of the mixed-allergen from house dust mites Dermatophagoides pteronyssinus and Dermatophagoides farinae for oral immunotherapy

Berzhets¹,

Koreneva²,

Khlgatian³

et al. 2019

Immunopathol Allergol Infectol

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Modern ideas about mechanisms of allergen-specific immunotherapy

Petrova

Юрьевна²,

Khlgatian

et al. 2021

Russian Journal of Allergy

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Reviews of domestic and foreign authors consider different approaches to understanding the formation of immunological and clinical tolerance induced by allergen-specific immunotherapy (ASIT). Despite the wide variety of theoretical research, the mechanism of the bodys immune systems response to ASIT remains unclear. The aim of this review is to analyze the current understanding of the mechanisms of formation of changes in the bodys reactivity in response to an allergen after ASIT. It is known that the type of response to the antigen is determined by its dose. In low-dose ASIT tolerance to the antigen is formed in the absence of inflammation, which is apparently associated with the activation of specific high-affinity receptors on cells of the immune system. High doses of allergen in ASIT probably lead to a rearrangement of cellular receptors, causing a decrease in their number by internalization or a weakening of their sensitivity to an excessive signal (desensitization). Due to a decrease in the number of receptors and / or their loss of sensitivity, the response to the antigen changes according to the principle of negative regulation, implemented at the level of receptor or postreceptor mechanisms. The formation of an anti-inflammatory cytokine response to antigen contributes to the differentiation of naive T cells into inducible regulatory T cells (iTreg). The suppressing effect of Treg on immune system cells affects Th effector cells, mast cells, basophils, eosinophils, B cells, and dendritic cells. The occurring immunological shifts form a new type of tolerant response to the allergen, namely, the change in the type of immunoglobulins from IgE to IgG and IgA and new phenotypes of T memory and B memory cells.

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Actual questions of standardization of water-salt extracts of house dust mites mixed allergens intended for medicinal forms of sublingual immunotherapy

Berzhets

Petrova

Нестеренко

et al. 2022

Russian Journal of Allergy

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Background: Standardization of medical forms of allergens is the process of establishing and applying a unified system of quality indicators and control methods. Quality control of therapeutic forms of allergens is an essential condition for guaranteeing the clinical efficacy and safety of allergen-specific immunotherapy (ASIT). The technology for obtaining the granular form of the mixed allergen from house dust mites Dermatophagoides pteronyssinus (Der p) and Dermatophagoides farinae (Der f) for oral use was developed in the laboratory of allergens Mechnikov Research Institute of Vaccines and Sera. Aims: to determine the authenticity of the anphenolic water-salt extract of the mixed allergen from mites Der p and Der f, intended for the therapeutic form of the drug. Materials and methods: Standardization of the obtained allergen extract was carried out by protein nitrogen unit by the Nessler method. The protein concentration was determined by the Bradford method. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was used to study the presence of protein composition in mixed allergen. The pH value was determined potentiometrically. The comparison of the specific activity of the standard allergen and the experimental mixed allergen was carried out by the method of reverse immunoassay (REAST). Results: SDS-PAGE showed the preservation of the protein profile of the water-salt extract of the mixed allergen Der p and Der f, corresponding to the protein profile of allergens of house dust mites. There were no statistically significant differences in the optical density of the studied allergen (four drug series) and the standard allergen with reference serums containing specific IgE to Der p or Der f when reacting with a Class 3 reference serum in REAST(р0,05). Conclusions: The above in accordance with the State Pharmacopoeia of the Russian Federation XIV edition is a confirmation of the authenticity of the drug. Not phenolic extraction did not affect the main allergenic components of the mixed allergen Der p and Der f and allowed it to maintain a high degree of specificity.

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Study of the Properties of the Mixed Water/Salt House Dust Mite Allergen Aiming to Design Its Sublingual Form

Cited by 3 publications

References 1 publication

Study of the specific activity of the granulated form of the mixed-allergen from house dust mites Dermatophagoides pteronyssinus and Dermatophagoides farinae for oral immunotherapy

Study of the specific activity of the granulated form of the mixed-allergen from house dust mites Dermatophagoides pteronyssinus and Dermatophagoides farinae for oral immunotherapy

Modern ideas about mechanisms of allergen-specific immunotherapy

Actual questions of standardization of water-salt extracts of house dust mites mixed allergens intended for medicinal forms of sublingual immunotherapy

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