Study on Angelica and its different extracts by Fourier transform infrared spectroscopy and two-dimensional correlation IR spectroscopy

Abstract

In order to develop a rapid and effective analysis method for studying integrally the main constituents in the medicinal materials and their extracts, discriminating the extracts from different extraction process, comparing the categories of chemical constituents in the different extracts and monitoring the qualities of medicinal materials, we applied Fourier transform infrared spectroscopy (FT-IR) associated with second derivative infrared spectroscopy and two-dimensional correlation infrared spectroscopy (2D-IR) to study the main constituents in traditional Chinese medicine Angelica and its different extracts (extracted by petroleum ether, ethanol and water in turn). The findings indicated that FT-IR spectrum can provide many holistic variation rules of chemical constituents. Use of the macroscopical fingerprint characters of FT-IR and 2D-IR spectrum can not only identify the main chemical constituents in medicinal materials and their different extracts, but also compare the components differences among the similar samples. This analytical method is highly rapid, effective, visual and accurate for pharmaceutical research.

Formation of Polyhydroxyalkanoate in Aerobic Anoxygenic Phototrophic Bacteria and Its Relationship to Carbon Source and Light Availability

“…The peak absorption band of the PHB standard, at about 1,728.6 cm Ϫ1 , corresponds to the ester carbonyl group of PHB. (25). At about 1,650 cm Ϫ1 is the vibration peak of the CAO bond of amides associated with proteins, usually called the amide I band, which may also contain contributions from CAC stretches of olefinic and aromatic compounds.…”

Evaluation of Phytoconstituents of Bryonopsis laciniosa fruit by UV-Visible Spectroscopy and FTIR analysis

“…The result confirms the occurrence of peaks at 208-881 nm reveals that the absorption bands are due to the presence of flavonoids, phenol and its derivatives in the bryonopsis laciniosa fruit. 29 FTIR Spectrum identified the functional group of the active chemical components present in the fruit based on the peak value in the region of infra red radiation. When the fruit extract was passed in to the FTIR, the functional group of the components was separated based on its ratio.…”

Peptide and Amino Acids Separation and Identification from Natural Products

“…These have often used reagents such as 2,4-dinitrofluorobenzene [2] and genipin [3]. Also, it has also been reported on the use of IR spectroscopy for the study of various extracts of Angelica [4].…”