Study on the angiogenesis ability of Polymethyl methacrylate-mineralized collagen/Mg-Ca composite material in vitro and the bone formation effect in vivo

Abstract

Magnesium (Mg) and its alloys show high degrees of biocompatibility and biodegradability, used as biodegrad able materials in biomedical applications. In this study, Polymethyl methacrylate (PMMA) - mineralized collagen (nano-Hydroxyapatite/collagen; nHAC)/Mg-Ca composite materials were prepared, to study the angiogenesis ability of its composite materials on Human umbilical vein endothelial cells (HUVECs) and its osteogenesis effect in vivo. The results showed that the PMMA-nHAC reinforcement materials can promote the proliferation and adhesion in HUVECs of Mg matrix significantly, it can enhance the migration motility and VEGF expression of HUVECs. In vivo, Micro-CT examination showed that with coated samples presenting the highest bone formation. Histologically, the materials and their corrosion products caused no systematic or local cytotoxicological effects. Therefore, the Mg matrix composites prepared in the present study has good biocompatibility and PMMA-nHAC/Mg-Ca composite may be an ideal orthopedic material to improve the bone formation, and biodegradable magnesium based implants with bioactivity have potential applications in bone tissue.

Composite Nanocoatings of Biomedical Magnesium Alloy Implants: Advantages, Mechanisms, and Design Strategies

“…[ 157 ] Nano‐mineralized collagen (nHAC) mixed with polymethyl methacrylate and CS and deposited on an Mg‐Ca alloy surface induced VEGF secretion and consequent EC proliferation and migration. [ 25,158 ] This coating facilitated angiogenesis and osteogenesis within four weeks of implantation in a rabbit calvarial defect model, while coatings without nHAC formed uneven nodular bone until 24 weeks after surgery. In addition to activating the VEGF signaling pathway, Au NPs [ 159 ] or NPs containing Zn 2+ or Sr 2+[ 160 ] upregulate Ang I through the Ang‐Tie signaling pathway to mediate EC migration, adhesion, and proliferation; thus, stimulating early angiogenesis.…”