Study on the extraction and purification of metabolites obtained from Trichoderma harzianum

Gupta, Bina Pani; Mathur, Abhishek; Koul, Akshma; Shrivastava, Vandana

doi:10.31024/ajpp.2019.5.3.16

Cited by 6 publications

(12 citation statements)

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“…The strains of F. oxysporum presented themselves more resistant to the antibiosis mechanisms when compared to the strains of F. solani . The general average inhibition observed was superior to 50% and the best results were connected to values above 70%, indicating that each species of Trichoderma possesses different abilities to inhibit different strains of Fusarium (Table 4) [1, 58, 61, 62].…”

Section: Resultsmentioning

confidence: 99%

Biodiversity and phylogeny of novel Trichoderma isolates from mangrove sediments and potential of biocontrol against Fusarium strains

et al. 2019

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Background: Studies carried out with novel 13 strains of Trichoderma, isolated from mangrove sediments (PE, Brazil) using morphophysiological and molecular characterization, followed evaluation of biocontrol using Fusarium strains isolated from Caatinga soil (PE, Brazil). Trichoderma strains were characterized by polyphasic taxonomic approach, and the extracted DNA was amplified with primers ITS 1 and 4, and sequenced. The biocontrol evaluation was conducted at 24 and 48 h of growth intervals by Tukey test, with a significance of 5%. Antibiosis tests were assessed in vitro by dual plate and partition plate techniques against Fusarium strains. Results: Trichoderma molecular identification, sequences of 500 bp were amplified, deposited into GenBank, and used for phylogenetic analyses. The strains were identified as T. asperellum (10), as T. harzianum (2) and one as T. longibrachiatum. Growth rate presented an average of 0.1207 cm h −1 for Trichoderma and lower growth rate of 0.031 cm h −1 for Fusarium spp., respectively. Antibiosis tests presented the best antagonist level of efficiency for T. asperellum UCP 0149 against F. solani UCP 1395 (82.2%) and F. solani UCP 1075 (70.0%), followed by T. asperellum UCP 0319 against F. solani UCP1083 (73.4%) and T. asperellum UCP 0168 against F. solani UCP1098 (71.5%), respectively. Conclusions: The data obtained in this study as tool for identification of novel Trichoderma strains serve as basis for development of several sustainable use for biotechnological processes. Those Trichoderma strains found promising for the management antagonistic potential and interaction could aid the conduct of biotechnological biocontrol of contaminants, and improve environmental conditions for the health of plants.

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Section: Resultsmentioning

confidence: 99%

Biodiversity and phylogeny of novel Trichoderma isolates from mangrove sediments and potential of biocontrol against Fusarium strains

et al. 2019

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“…Ag*NP have a unique electrical, thermal, optical & biological properties [12,13]. It is the reason that silver Nanoparticle has been introduced in practical application like molecular diagnostics and a trial of its fused version with natural antimicrobial agent is undergoing research to find out the pharmaceutical approach in the field of clinical sciences [14]. The basic chemistry behind Ag*NP is when this nanoparticle is in solution, molecules associated with the nanoparticle surface to establish a double layer of charge that stabilize the particle and prevent aggregation [15,16].…”

Section: Silver: Properties and Usementioning

confidence: 99%

“…Silver nanoparticle are being used in numerous technologies and incorporated into a wide array of consumer products and take benefits of their desirable optical conductive and antibacterial properties [14,17]. Ag*NP have diagnostic application used as biological tags for quantitative detection, antibacterial application like Ag*NP are incorporated in apparel, footwear, wound dressing & bactericidal & bacteriostatic capability [16].…”

Section: Silver: Properties and Usementioning

confidence: 99%

“…Trichoderma kongii prepared Ag*NP treated against S.typhimurium, 45 µg/ml inhibit its growth [9]. Trichoderma first came into known as antimicrobial agent by Christian Hendrik Persoon and its quality is it is fast growing [12,14].…”

Section: Approx Same Absorbance Peak Observed Bymentioning

confidence: 99%

“…There are list of organism that have ability of forming Ag*NP that is T. resai, T. viridae, T. harzianum, T. Koningii, F. oxysporangium, Alternana Alternata, A. Terrus, T. Virens, T. aspergillum, Phomaglamerata, T. longibraditum [14,22]. There are many approaches of nanoparticle of silver antibacterial, antiviral, antifungal, anti inflammatory, antiangiogenic, antitumor.…”

Section: Approx Same Absorbance Peak Observed Bymentioning

confidence: 99%

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Standardization, Characterization and Isolation of Trichoderma-Silver Nanoparticle-A Pharmaceutical Approach in Field of Nano-Medicine

Gupta¹,

Gaur²

2021

JPRI

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Silver is a well known antimicrobial agent. It is utilized in many antimicrobial and medication. The Trichoderma and its Metabolites have also been meant for antimicrobial activity against the various bacterial and fungal strain. In the present investigation Nanoparticle of silver is prepared by chemical method, green synthesis using plants and biosynthesis using microbes. Trichoderma secondary metabolite prepared by solvent extraction method from Trichoderma harzianum which act as a capping and reducing agent. The biosynthesised silver Nanoparticles were characterized by UV-Vis spectroscopy and TEM. UV-Vis spectra of silver Nanoparticle and trichoderma extract showed absorption spectra at 420nm & 430 nm respectively while the fused nanoparticle with Trichoderma secondary metabolite showed absorption spectra at 415 nm corresponding to the surface Plasmon resonance of silver Nanoparticle. It was determined the nanoparticle showed absorption spectra at 415 nm and morphology as spherical with size range 8 to 24 nm and providing good antimicrobial activity as Trichoderma silver fused nanoparticle against many microbial strain, so it can be prepared for pharmaceutical approach against the infectious disease caused by clinical pathogenic organisms.

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Concurrent application of bacterial-mediated and mycosynthesized ZnO nanofungicides to maintain high ascorbic acid and delay postharvest decay of apricot

Farhana

Ali

Akbar

et al. 2023

Microbial Pathogenesis

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Study on the extraction and purification of metabolites obtained from Trichoderma harzianum

Cited by 6 publications

References 0 publications

Biodiversity and phylogeny of novel Trichoderma isolates from mangrove sediments and potential of biocontrol against Fusarium strains

Biodiversity and phylogeny of novel Trichoderma isolates from mangrove sediments and potential of biocontrol against Fusarium strains

Standardization, Characterization and Isolation of Trichoderma-Silver Nanoparticle-A Pharmaceutical Approach in Field of Nano-Medicine

Concurrent application of bacterial-mediated and mycosynthesized ZnO nanofungicides to maintain high ascorbic acid and delay postharvest decay of apricot

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