Study on the fingerprint profile of Monascus products with HPLC–FD, PAD and MS

Accumulation of yellow Monascus pigments by extractive fermentation in nonionic surfactant micelle aqueous solution

“…The concentration of Monascus pigments is usually represented by the absorbance at their characteristic wavelength (Babitha et al 2007). However, there is overlap among the absorbance spectrum of single component in Monascus pigments (Zheng et al 2009) and the composition of Monascus pigments is always changed with the various culture conditions (Kang et al 2013a), which makes the representation of pigment concentration by color value inaccurate. Thus, the visible spectrum of Monascus pigments was determined.…”

“…Different from submerged culture in the aqueous solution, most of Monascus pigments had been exported into its broth by extractive fermentation in the nonionic surfactant micelle aqueous solution. At the same time, the absorbance spectra of both intracellular and extracellular Monascus pigments had one peak at approximately 410 nm, which were consisted with the characteristic absorbance of yellow Monascus pigments (Zheng et al 2009). It should be stressed that the total absorbance of cell interiors and extracellular broth at 410 nm in the extractive fermentation was much higher than that of submerged culture in the aqueous solution.…”

“…Monascus pigments were majorly accumulated intracellularly by submerged culture in the aqueous solution. The spectrum of intracellular Monascus pigments had a board absorbance with one peak at approximately 470 nm, which exhibited the characteristic absorbance of orange Monascus pigments (Zheng et al 2009). Different from submerged culture in the aqueous solution, most of Monascus pigments had been exported into its broth by extractive fermentation in the nonionic surfactant micelle aqueous solution.…”

Identification of components in Zhi-Zi-Da-Huang decoction by HPLC coupled with electrospray ionization tandem mass spectrometry, photodiode array and fluorescence detectors

“…1: FD for peaks 4, 7 ( ex 329 nm/ em 446 nm), 25, 27, 28, 29 and 30 ( ex 440 nm/ em 520 nm), MS-TIC for peaks 5,6,8,9,11,12,13,14,16,17,19,21,23,24 and 26, MS-EIC for peaks 1, 2, 3, 10, 15, 18, 20 and 22.…”

Simultaneous determination of two Monascus metabolites in red yeast rice by HPLC using fluorescence detection

“…1a. The two Monascus metabolites were also reported in a literature (Zheng, Xin, & Guo, 2009). However, there are no detailed studies available concerning the distribution of MFA and MFB in red yeast rice or the analytical methods used for their determination in red yeast rice samples.…”