Honey nanoparticles have shown numerous unique characteristics such as biocompatibility and are excellent agents for biological applications. This study aims to investigate honey nanoparticles for 2, 2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical scavenging activity and their ability to offer protection against gamma radiation. In vitro antioxidant activity study of honey nanoparticles has shown good free radical scavenging activity for DPPH radical assayed within a concentration range of (0.05–2.5)[Formula: see text]×[Formula: see text][Formula: see text][Formula: see text]g/l, at higher concentrations of nanoparticles showed reverse trend in absorbance and inhibition indicating this finite range of concentration is suitable for scavenging free radicals, also nanoparticles were found to have significant antioxidant capacity and thus can be used as potential radical scavenger against deleterious damages caused by the free radicals.