Subcellular localization of the <i>Bacillus subtilis</i> structural maintenance of chromosomes (SMC) protein

Lindow, Janet C.; Kuwano, Masayoshi; Moriya, Shigeki; Grossman, Alan D.

doi:10.1046/j.1365-2958.2002.03235.x

Cited by 73 publications

(72 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5 B, iii and iv). The distribution of EptC loci is consistent with the distribution of SMC/MukB protein in other prokaryotes (37).…”

Section: Bioinformatic Analysis Of Eptc Reveals Homology To Smc Protesupporting

confidence: 82%

“…This consistent mobility pattern irrespective of oligonucleotide sequences showed that like other SMCs, EptC bound to ssDNA in a dose-dependent but sequenceindependent fashion. Efficient segregation of low-copy plasmids in E. coli is affected by plasmid topology (36), and SMC proteins are well known to alter DNA topology after binding to DNA (7,28,37). A coupled topoisomerase assay was used to examine the effect of EptC on DNA topology (38).…”

Section: Bioinformatic Analysis Of Eptc Reveals Homology To Smc Protementioning

confidence: 99%

See 1 more Smart Citation

Noncanonical SMC protein in Mycobacterium smegmatis restricts maintenance of Mycobacterium fortuitum plasmids

Panas

Jain

Yang

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Research on tuberculosis and leprosy was revolutionized by the development of a plasmid transformation system in the fast-growing surrogate, Mycobacterium smegmatis. This transformation system was made possible by the successful isolation of a M. smegmatis mutant strain mc 2 155, whose efficient plasmid transformation (ept) phenotype supported the replication of Mycobacterium fortuitum pAL5000 plasmids. In this report, we identified the EptC gene, the loss of which confers the ept phenotype. EptC shares significant amino acid sequence homology and domain structure with the MukB protein of Escherichia coli, a structural maintenance of chromosomes (SMC) protein. Surprisingly, M. smegmatis has three paralogs of SMC proteins: EptC and MSMEG_0370 both share homology with Gramnegative bacterial MukB; and MSMEG_2423 shares homology with Gram-positive bacterial SMCs, including the single SMC protein predicted for Mycobacterium tuberculosis and Mycobacterium leprae. Purified EptC was shown to bind ssDNA and stabilize negative supercoils in plasmid DNA. Moreover, an EptC-mCherry fusion protein was constructed and shown to bind to DNA in live mycobacteria, and to prevent segregation of plasmid DNA to daughter cells. To our knowledge, this is the first report of impaired plasmid maintenance caused by a SMC homolog, which has been canonically known to assist the segregation of genetic materials.plasmid segregation | electroporation | DNA topology | partition errors | cell division

show abstract

“…5 B, iii and iv). The distribution of EptC loci is consistent with the distribution of SMC/MukB protein in other prokaryotes (37).…”

Section: Bioinformatic Analysis Of Eptc Reveals Homology To Smc Protesupporting

confidence: 82%

Section: Bioinformatic Analysis Of Eptc Reveals Homology To Smc Protementioning

confidence: 99%

Noncanonical SMC protein in Mycobacterium smegmatis restricts maintenance of Mycobacterium fortuitum plasmids

Panas

Jain

Yang

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Heat stable b-galactosidase activity of the bgaB gene from B. stearothermophilus was measured at 62 uC as described elsewhere (Yuan & Wong, 1995;Platt et al, 1972;Sadaie, 1998). Lindow et al (2002) Samples were heated at 68 uC for 5 min before measuring BgaB activity, to inactivate the endogenous b-galactosidase of the lacZ gene of the E. coli in the integrated pMutin plasmid.…”

Section: Methodsmentioning

confidence: 99%

Regulatory role of RsgI in sigI expression in Bacillus subtilis

et al. 2007

View full text Add to dashboard Cite

The sigma gene, sigI, of Bacillus subtilis belongs to the group IV heat-shock response genes and has many orthologues in the bacterial phylum Firmicutes. The B. subtilis sigI gene is considered to constitute an operon with rsgI (regulation of sigI, formerly ykrI). As little is known about either the structure and function of the sigI-rsgI operon or the SigI regulons, the role of RsgI in heat-inducible transcription of the sigI-rsgI operon was investigated, using Northern analysis and a heat-stable b-galactosidase reporter assay. Heat-inducible, SigI-dependent transcription of the sigI-rsgI operon was stimulated greatly by disrupting rsgI. Yeast two-hybrid analysis showed direct interaction between the N-terminal portion of the presumed RsgI protein and SigI. Without RsgI function, induction of transcription of the sigI-rsgI operon upon transient heat stress depended on dnaK activity. However, transcription of the operon was induced during growth at prolonged higher temperature even without DnaK function. Without RsgI function, sigI-rsgI operon transcription was induced after the end of growth independent of any temperature shift in a sporulation medium and toward the end of growth in a rich complex medium. Furthermore, glucose addition resulted in a strong suppression of sigI-rsgI transcription. Therefore it is hypothesized that transcription of the sigI-rsgI operon of B. subtilis is negatively regulated by the putative transmembrane protein RsgI, which moderates SigI's sensitivity to heat shock or nutritional stress.

show abstract

“…Overexpression of MukB has been observed to cause chromosome overcondensation in vivo (Wang et al 2006). Estimates of 1000 bsSMC condensins in B. subtilis (Lindow et al 2002) suggest that there is roughly one bacterial condensin per 10 kb of (replicated) DNA, not terribly different from the eukaryote ratio. SMCs are also found in archaeal species (Hirano 2005), making them a chromosomal protein that can be found in all three domains of life.…”

Section: Universality Of Smc Complexesmentioning

confidence: 99%

Micromechanical studies of mitotic chromosomes

2008

View full text Add to dashboard Cite

Mitotic chromosomes respond elastically to forces in the nanonewton range, a property important to transduction of stresses used as mechanical regulatory signals during cell division. In addition to being important biologically, chromosome elasticity can be used as a tool for investigating the folding of chromatin. This paper reviews experiments studying stretching and bending stiffness of mitotic chromosomes, plus experiments where changes in chromosome elasticity resulting from chemical and enzyme treatments were used to analyse connectivity of chromatin inside chromosomes. Experiments with nucleases indicate that non-DNA elements constraining mitotic chromatin must be isolated from one another, leading to the conclusion that mitotic chromosomes have a chromatin Fnetwork_ or Fgel_ organization, with stretches of chromatin strung between Fcrosslinking_ points. The as-yet unresolved questions of the identities of the putative chromatin crosslinkers and their organization inside mitotic chromosomes are discussed.

show abstract

Subcellular localization of the Bacillus subtilis structural maintenance of chromosomes (SMC) protein

Cited by 73 publications

References 68 publications

Noncanonical SMC protein in Mycobacterium smegmatis restricts maintenance of Mycobacterium fortuitum plasmids

Noncanonical SMC protein in Mycobacterium smegmatis restricts maintenance of Mycobacterium fortuitum plasmids

Regulatory role of RsgI in sigI expression in Bacillus subtilis

Micromechanical studies of mitotic chromosomes

Contact Info

Product

Resources

About