Subcellular Organization of the Developing Cotyledons of Pisum Sativum L.

Bain, Joan M; Mercer, FV

doi:10.1071/bi9660049

Cited by 143 publications

(56 citation statements)

References 30 publications

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“…We reported earlier that during cotyledon development of V. faba the principal sugars change from a high hexose-to-sucrose ratio to high concentrations of sucrose . Interestingly, there possibly exists a relationship between sugar content and the subcellular organisation of the vacuolar system (Bain and Mercer 1966;Goodchild and Craig 1982). Goodchild and Craig (1982) speculated that the appearance of proteins inside the vacuole, as well as the formation of protein bodies, may be in¯uenced by the sugar levels in the cotyledons.…”

Section: Discussionmentioning

confidence: 98%

“…Later on, the vegetative vacuole disappears by a currently unknown mechanism. According to early investigations by Bain and Mercer (1966), Craig (1988) and Craig et al (1979) the vegetative vacuole is thought to be continuously ®lled with storage proteins and then to fragment into small protein bodies. Other investigators have proposed that the storage proteins are not deposited into existing vegetative vacuoles but into newly formed protein-storage vacuoles.…”

Section: Introductionmentioning

confidence: 99%

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Sugar levels altered by ectopic expression of a yeast-derived invertase affect cellular differentiation of developing cotyledons of Vicia narbonensis L.

Neubohn

Gubatz

Wobus

et al. 2000

Planta

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In order to change the sugar status during seed development a yeast-derived invertase gene was expressed in cotyledons of Vicia narbonensis. As a result, sucrose decreased whereas hexoses accumulated. We analysed cell structure and cellular differentiation in cotyledons expressing the yeast-invertase. Transgenic cells contained large and long-persisting vacuoles apparently serving as storage compartments for hexoses and clusters of storage-protein aggregates. In the wild-type, large vacuoles did not persist but were replaced by smaller protein bodies. During maturation and desiccation, the transgenic cells showed plasmolysis and vesiculation of the endo-membrane system. Immunogold-labelling revealed that the storage proteins vicilin and legumin were present within the cytoplasm and the extraprotoplasmic space and were attached to membranes of the endoplasmic reticulum and the nuclei. Protein storage vacuoles in mature seeds appeared heterogeneous and only partially filled. The data suggest that sugars control the subcellular organisation of the vacuolar system. Transcript levels encoding a tonoplast intrinsic protein, a marker for membranes of protein storage vacuoles, remained unchanged whereas mRNA levels of a hexose and a sucrose transporter increased. Generally, transgenic seeds appeared to be physiologically younger than wild-type seeds of the same age. The data underline the important role of sugars in legume seed development.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Sugar levels altered by ectopic expression of a yeast-derived invertase affect cellular differentiation of developing cotyledons of Vicia narbonensis L.

Neubohn

Gubatz

Wobus

et al. 2000

Planta

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“…In Pisum (pea), as is the case with many other grain legumes, seed storage proteins are synthesised only in parenchyma cells of developing cotyledons, during the cell expansion phase of embryogenesis, when other storage products (starch and lipid) are also synthesised [ 13,46]. The majority of pea storage protein is soluble in dilute salt solution and therefore classified as globulin [43] and is sequestered in membrane-bound vesicles known as protein bodies [2,15]. On the basis of their sedimentation [20] storage proteins in Pisum may be classified into 11S (legumin) and 7S (vicilin) fractions.…”

Section: Introductionmentioning

confidence: 99%

The effect of different alleles at the r locus on the synthesis of seed storage proteins in Pisum sativum

Turner

Barratt²,

Casey

1990

Plant Mol Biol

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Rocket immunoelectrophoresis was used to measure the accumulation of storage proteins in developing cotyledons of two Pisum sativum (pea) genotypes, that were close to isogenic except for the nature of the allele at the r locus. There was a marked decrease in legumin accumulation in the rr (wrinkled-seeded) genotype compared to the RR (round-seeded) genotype. The accumulation of vicilin did not differ greatly between the two genotypes. Pulse-labelling studies indicated that the differences in rates of accumulation of legumin between the rr and RR genotypes were a consequence of differences in rates of protein synthesis. Measurements of relative amounts of specific mRNAs, using cDNA clones as probes, showed lower amounts of legumin mRNA in developing cotyledons of the rr, compared to the RR, genotype. Both vicilin mRNAs and convicilin mRNA, the latter of which shows a similar temporal pattern of expression to those of the major legumin species, are relatively unaffected by the nature of the allele at the r locus. Nuclear run-on transcription experiments indicated no differences in the rate of synthesis of legumin transcripts in the rr and RR near-isolines. The consequences of homozygosity for the r allele on storage protein mRNA levels in vitro may be mimicked by manipulating the sucrose concentration of the culture medium.

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“…During later stages and maturation of seeds, food reserved in the form of lipids, carbohydrates, and proteins was laid down in the cotyledons. Ultrastructural studies of Phaseolus vulgaris [40], Vicia faba [41], and P. sativum [42] showed the pattern of multivasiculation in the cotyledon cells. The albumin fraction continuously increased from 5th to 25th day (DAF), and globulin proteins started synthesizing at later stages from the tenth day, and the maximum amount was observed at the 20th day; after that, its synthesis decreased gradually.…”

Section: Seed Storage Proteins Fractionation and Analysismentioning

confidence: 98%

Heterologous Expression of Legumin Gene in E. coli Isolated from cDNA Clones of Immature Seeds of Pigeonpea (Cajanus cajan L.)

Gupta

Mishra

Verma

et al. 2008

Appl Biochem Biotechnol

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Proteins are one of the targets for improving the nutritional quality, and attempts are being made through manipulation of its native gene(s). Pigeonpea (Cajanus cajan L.) is one of the nutritionally important legumes of tropical and subtropical regions of the world, and studies of the structure of seed storage proteins and their interactions have been limited by the difficulty of isolating single-protein subunits in large amounts from a complex mixture of the seed endosperm. One way to overcome this problem is the expression of seed storage protein-encoded gene(s) in heterologous systems that have additional advantages wherein specific gene modifications can be made and the new gene constructs can quickly be expressed. Legumin protein was extracted from pigeonpea seeds of different developmental stages (5th to 25th day after flowering [DAF]) and characterized. The legumin gene (leg) of size 1.482 kb was screened, using the deoxygenin-labeled legumin probe, from the complementary deoxyribonucleic acid (cDNA) library, constructed from 18-day-old (DAF) immature seeds of pigeonpea and sequenced (accession no. AF3555403). The legumin gene was further characterized by DNA blotting, and its probable secondary structure was predicted using online ExPASy server. Significant Protein Data Bank (PDB) alignment of the deduced legumin protein by BLASTP was observed with proglycinin of soybean. Comparative 3D structural homology was predicted by Cn3D software, and the legumin protein showed the 3D structure alignment and interaction homology with proglycinin chain 1FXZA (PDB no. 1FXZ). The legumin gene was subcloned in vector pET-24a driven by the bacterial promoter, and its expression was detected in Escherichia coli by immunoblotting using polyclonal antibodies, raised against the purified legumin protein.

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Subcellular Organization of the Developing Cotyledons of Pisum Sativum L.

Cited by 143 publications

References 30 publications

Sugar levels altered by ectopic expression of a yeast-derived invertase affect cellular differentiation of developing cotyledons of Vicia narbonensis L.

Sugar levels altered by ectopic expression of a yeast-derived invertase affect cellular differentiation of developing cotyledons of Vicia narbonensis L.

The effect of different alleles at the r locus on the synthesis of seed storage proteins in Pisum sativum

Heterologous Expression of Legumin Gene in E. coli Isolated from cDNA Clones of Immature Seeds of Pigeonpea (Cajanus cajan L.)

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