Subclinical mastitis in dairy cows: somatic cell counts and associated bacteria in Mymensingh, Bangladesh

Sumon, Smmr; Ehsan, M. A.; Islam, Taohidul

doi:10.3329/jbau.v15i2.35073

Cited by 21 publications

(29 citation statements)

References 22 publications

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“…Although the provision of SCC is readily available to most dairy farms in developed countries on a monthly basis through the dairy herd improvement (DHI) program, this practice yet not accustomed in Bangladesh. Because of the high prevalence (30-72%) of SCM and abundance of the mastitis pathogens [14][15][16][17][18][19], the provision of SCCs for routine SCM screening in dairy herds will be of great benefit in reducing the occurrence of SCM and ensuring milk quality as well.…”

Section: Introductionmentioning

confidence: 99%

Relationship between somatic cell counts and subclinical mastitis in lactating dairy cows

et al. 2020

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Background and Aim: Subclinical mastitis (SCM) is one of the most prevalent diseases of dairy cows, and somatic cell count (SCC) is widely used to determine SCM and milk quality. However, setting the threshold of SCC is very important. This study aimed to determine the cow-level threshold of SCC to differentiate milk of SCM affected cows from normal milk in Bangladesh. Materials and Methods: Sensitivity (Se) and specificity (Sp) along with other characteristics of different thresholds of SCC were determined considering the bacterial culture as the gold standard test. Three definitions of intramammary infection (IMI) were set based on the group of pathogens involved. Five categories of SCC thresholds were considered for analysis. Results: Se and Sp of thresholds of SCC greatly varied as definitions of IMI changed. Irrespective of SCC thresholds, Se increased when isolation of major pathogens included in IMI definitions. Se decreased when SCC thresholds increased (from 100 to 300 × 103 cells/mL) for all IMI definitions and ranged from 60.6% to 20.3%. The highest Se was found at low SCC threshold (100 × 103 cells/mL), which resulted in less false-negative outcome. On the other hand, Sp increased with the increment of SCC thresholds giving rise to a less false-positive outcome. Conclusion: The cow-level SCC threshold of 100 × 103 cells/mL was found appropriate to identify subclinical IMI of dairy cows.

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Section: Introductionmentioning

confidence: 99%

Relationship between somatic cell counts and subclinical mastitis in lactating dairy cows

et al. 2020

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“…In another study, we found the CMT as an effective test kit for assessing SCM [13]. However, Sarker et al [23] and Sumon et al [17] found only 20.2% and 25% prevalence of SCM in dairy cows, respectively. A medium ranged prevalence of SCM (50.4% and 58%, respectively) was reported by Tripura et al [24] and Mpatswenumugabo et al [25].…”

Section: Resultsmentioning

confidence: 93%

“…Thus, prevention and control of mastitis is a big challenge throughout the world. Several researchers have reported the prevalence, potential risk factors, and comparison of different screening tests for bovine mastitis in Bangladesh [10–17]. However, the screening tests of SCM using commercially available foreign kits need huge money.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of BLRI Mastitis Test Kit at Bangladesh Livestock Research Institute Regional Station, Sirajganj

et al. 2019

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Objective:The objective of this study was to develop a low-cost kit for the detection of subclinical mastitis (SCM) and to check its validity, reproducibility, and efficacy at the field level.Materials and Methods:A total of 550 quarter milk samples from crossbred dairy cows were collected, of which 400 milk samples were used to validate the newly developed BLRI mastitis test (BMT) kit to justify its efficacy as an individual test kit in detecting SCM based on somatic cell count (SCC) by direct microscopic count (DMC). The efficacy of the newly developed BMT was compared with the California Mastitis Test (CMT) kit. Another 150 milk samples were subjected to SCC determined by DMC and DCC (De Laval cell counter®) categorized by CMT and BMT scores.Results:A SCM test kit, namely, BMT kit was successfully developed in this study. The percentage accuracy of CMT and BMT were 76.75% and 75.75%; sensitivity 69.36% and 67.56%; specificity 85.95% and 85.85%; positive predictive value 86.03% and 85.71%; negative predictive value 69.23% and 68%, respectively. A p value of 0.001 was found for both CMT and BMT. However, CMT and BMT had no significant difference in sensitivity (p = 0.778). Average SCCs (cells/ml) determined by DCC and DMC, respectively, were mostly corresponded to the SCC ranges of both CMT and BMT scores.Conclusion:The newly developed BMT kit is an independent, cheap, farmer-friendly, first country made, and reliable SCM diagnostic test kit that can be used at field condition.

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“…Enterobacter spp has been reported from previous studies (Sumon et al, 2017) and are important agents of environmental intramammary infection and are mostly sourced from farm manure, bedding, and soil (Swartz and Christina, 2016).…”

Section: Discussionmentioning

confidence: 97%

Prevalence and Antimicrobial Resistance Profiles of Bacterial Isolates Associated with Intramammary Infections in Malaysian Dairy Herds

Zunita

Jesse

Ramanoon

et al. 2020

Preprint

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Intramammary infections are costly endemic disease in dairy cows and it highly affects both the quality and quantity of milk production in addition to the animal well-being. It a major cause of considerable economic losses to the dairy farmers. This study was aimed to determine the prevalence of mastitis pathogens and antimicrobial resistance pattern of selected most prevalent pathogens. A total of 1945 quarter samples from 517 cows across 33 dairy herds were used. Isolation and identification of mastitis pathogens was carried out based on standard bacteriological procedures. Antimicrobial resistance profile was conducted by disk diffusion technique. The overall culture prevalence was 67.6% (n=503/744, CI= 64.1-70.9). The respective states’ culture prevalence were 66.4% (n=71/107, CI= 56.5-75.0), 60.3% (184/305, CI= 54.6-65.8), 58% (n=94/162, CI= 50-65.6), 100% (n=75/75, CI= 93.9-100), and 83.3% (n=79/95, CI= 73.8-89.8) for Pahang, Perak, Selangor, Negeri Sembilan and Johor respectively. The most prevalent isolates identified were non-aureus staphylococci (NAS) 39.5%, S. aureus 13.1%, K. pneumoniae 6.5%, S. agalactiae 4.8%, and S. uberis 4.3%. Resistance profile of K. pneumoniae isolates against ampicillin and Penicillin G were 70.4% and 88.9% respectively while that of Actinobacter spp against chloramphenicol and streptomycin were 71.4% and 35.7% respectively. For S. uberis isolates, 66.7% and 73.3% have shown resistance to tetracycline and streptomycin respectively. This study provides knowledge of the epidemiology of bacterial isolates associated with subclinical mastitis in Malaysia and their resistance profile. The high prevalence of these pathogens in milk and multidrug resistance observed should not be over looked.

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Subclinical mastitis in dairy cows: somatic cell counts and associated bacteria in Mymensingh, Bangladesh

Cited by 21 publications

References 22 publications

Relationship between somatic cell counts and subclinical mastitis in lactating dairy cows

Relationship between somatic cell counts and subclinical mastitis in lactating dairy cows

Development and validation of BLRI Mastitis Test Kit at Bangladesh Livestock Research Institute Regional Station, Sirajganj

Prevalence and Antimicrobial Resistance Profiles of Bacterial Isolates Associated with Intramammary Infections in Malaysian Dairy Herds

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