Submicron 3,4-dihydroxybenzoic acid–TiO<sub>2</sub> composite particles for enhanced MALDI MS imaging of secondary metabolites in the root of differently aged baical skullcap

Sun, Ruiyang; Zhang, Ying; Tang, Weiwei; Li, Bin

doi:10.1039/d2an00710j

Cited by 10 publications

(4 citation statements)

References 35 publications

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“…Sources which do not require matrix application are less popular due to their lower versatility compared to standard sources. MALDI is usually used for large molecules (i.e., proteins), but it works also well for smaller organic compounds and sporadically can be employed for inorganic materials [ 27 ]. The principle of MALDI is mixing the sample with the matrix, co-crystallization at ambient conditions, and loading the sample into a vacuum, where the molecules are desorbed and ionized with the laser beam (see Figure 1 A).…”

Section: Techniques Used For Biomaterials Analysismentioning

confidence: 99%

Mass Spectrometry Imaging of Biomaterials

Kret,

Bodzon-Kulakowska,

Drabik

et al. 2023

Materials

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The science related to biomaterials and tissue engineering accounts for a growing part of our knowledge. Surface modifications of biomaterials, their performance in vitro, and the interaction between them and surrounding tissues are gaining more and more attention. It is because we are interested in finding sophisticated materials that help us to treat or mitigate different disorders. Therefore, efficient methods for surface analysis are needed. Several methods are routinely applied to characterize the physical and chemical properties of the biomaterial surface. Mass Spectrometry Imaging (MSI) techniques are able to measure the information about molecular composition simultaneously from biomaterial and adjacent tissue. That is why it can answer the questions connected with biomaterial characteristics and their biological influence. Moreover, this kind of analysis does not demand any antibodies or dyes that may influence the studied items. It means that we can correlate surface chemistry with a biological response without any modification that could distort the image. In our review, we presented examples of biomaterials analyzed by MSI techniques to indicate the utility of SIMS, MALDI, and DESI—three major ones in the field of biomaterials applications. Examples include biomaterials used to treat vascular system diseases, bone implants with the effects of implanted material on adjacent tissues, nanofibers and membranes monitored by mass spectrometry-related techniques, analyses of drug-eluting long-acting parenteral (LAPs) implants and microspheres where MSI serves as a quality control system.

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Section: Techniques Used For Biomaterials Analysismentioning

confidence: 99%

Mass Spectrometry Imaging of Biomaterials

Kret,

Bodzon-Kulakowska,

Drabik

et al. 2023

Materials

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“…11 Currently, it is worth mentioning that inorganic nanomaterial matrices, such as platinum nanomaterial, 14 and carbon-based nanoparticles 15 have attracted much attention due to their few background ions. 16 Nevertheless, the drawbacks of inorganic matrices are also obvious, such as low sensitivity, poor reproducibility, high cost, and some damage to the mass spectrometers. 17 In comparison, the application of organic matrices is preferred.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Moreover, an ideal matrix has few matrix-related ion signals, strong absorption at the laser operating wavelength, and high ionization efficiency for the analytes . Currently, it is worth mentioning that inorganic nanomaterial matrices, such as platinum nanomaterial, and carbon-based nanoparticles have attracted much attention due to their few background ions . Nevertheless, the drawbacks of inorganic matrices are also obvious, such as low sensitivity, poor reproducibility, high cost, and some damage to the mass spectrometers .…”

Section: Introductionmentioning

confidence: 99%

2-Hydroxy-5-nitro-3-(trifluoromethyl)pyridine as a Novel Matrix for Enhanced MALDI Imaging of Tissue Metabolites

Bao,

Yu,

et al. 2024

Anal. Chem.

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Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), which is a label-free imaging technique, determines the spatial distribution and relative abundance of versatile endogenous metabolites in tissues. Meanwhile, matrix selection is generally regarded as a pivotal step in MALDI tissue imaging. This study presents the first report of a novel MALDI matrix, 2-hydroxy-5-nitro-3-(trifluoromethyl)pyridine (HNTP), for the in situ detection and imaging of endogenous metabolites in rat liver and brain tissues by MALDI-MS in positive-ion mode. The HNTP matrix exhibits excellent characteristics, including strong ultraviolet absorption, μm-scale matrix crystals, high chemical stability, low background ion interference, and high metabolite ionization efficiency. Notably, the HNTP matrix also shows superior detection capabilities, successfully showing 185 detectable metabolites in rat liver tissue sections. This outperforms the commonly used matrices of 2,5-dihydroxybenzoic acid and 2-mercaptobenzothiazole, which detect 145 and 120 metabolites from the rat liver, respectively. Furthermore, a total of 152 metabolites are effectively detected and imaged in rat brain tissue using the HNTP matrix, and the spatial distribution of these compounds clearly shows the heterogeneity of the rat brain. The results demonstrate that HNTP is a new and powerful positive-ion mode matrix to enhance the analysis of metabolites in biological tissues by MALDI-MSI.

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“…22 ) In recent years, many research groups have used this technology to study the biological macromolecules of living organisms, for instance, MALDI imaging analysis of breviscapine and its metabolites in mouse kidneys 23 and visualizing the spatial distribution of endogenous molecules in wolfberry fruit at different development stages. 24 Although MALDI-MSI has become popular in the detection of biological macromolecules, its capability to analyze low molecular weight compounds ( m / z < 500) is limited because of the interferences of the added organic matrix in this mass range. It is easy to interfere the mass spectral peaks when low molecular weight compounds are detected in biological tissues.…”

Section: Introductionmentioning

confidence: 99%

Visualizing the distribution of curcumin in the root of Curcuma longa via VUV-postionization mass spectrometric imaging

Nie

et al. 2023

Analyst

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Submicron 3,4-dihydroxybenzoic acid–TiO₂ composite particles for enhanced MALDI MS imaging of secondary metabolites in the root of differently aged baical skullcap

Abstract: This work provides a high-efficient organic-inorganic hybrid matrix for MALDI MSI of secondary metabolites in plant tissues.

Cited by 10 publications

References 35 publications

Mass Spectrometry Imaging of Biomaterials

Mass Spectrometry Imaging of Biomaterials

2-Hydroxy-5-nitro-3-(trifluoromethyl)pyridine as a Novel Matrix for Enhanced MALDI Imaging of Tissue Metabolites

Visualizing the distribution of curcumin in the root of Curcuma longa via VUV-postionization mass spectrometric imaging

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Submicron 3,4-dihydroxybenzoic acid–TiO2 composite particles for enhanced MALDI MS imaging of secondary metabolites in the root of differently aged baical skullcap

Abstract: This work provides a high-efficient organic-inorganic hybrid matrix for MALDI MSI of secondary metabolites in plant tissues.

Cited by 10 publications

References 35 publications

Mass Spectrometry Imaging of Biomaterials

Mass Spectrometry Imaging of Biomaterials

2-Hydroxy-5-nitro-3-(trifluoromethyl)pyridine as a Novel Matrix for Enhanced MALDI Imaging of Tissue Metabolites

Visualizing the distribution of curcumin in the root of Curcuma longa via VUV-postionization mass spectrometric imaging

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Product

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Submicron 3,4-dihydroxybenzoic acid–TiO₂ composite particles for enhanced MALDI MS imaging of secondary metabolites in the root of differently aged baical skullcap