Substance P-Induced Histamine Release from Human Basophils, Skin and Lung Fragments: Effect of Nedocromil Sodium and Theophylline

Louis, Renaud; Radermecker, Maurice

doi:10.1159/000235160

Cited by 34 publications

(16 citation statements)

References 14 publications

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“…In our experimen tal conditions, it was not possible to construct a complete dose-rcsponsc curve for each subject because 3 different sccretagogucs were tested on the same blood sample. Previous studies from our labora tory have, however, shown that antihuman IgE diluted to contain 0.25 ug antibodics/ml and FMP I0-1 M were optimal concentrations for leukocyte histamine release [5]. In preliminary experiments, we first determined the concentration of venom causing maximum specific (i.c.…”

Section: Leukocyte Releasabilitymentioning

confidence: 99%

Basophil Releasability in Patients with Hymenoptera Venom Allergy

Radermecker

Leclercq

Mariz³

et al. 1993

Int Arch Allergy Immunol

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Basophils in about 15% of subjects allergic to hymenoptera venom do not release histamine in the presence of antigen. Little is known on the basophil releasability in these patients. We therefore measured maximum percent leukocyte histamine release to antigen (Vespula venom), anti-IgE and formylmethionylphenylalanine (FMP) in 39 patients allergic to wasp venom and compared our results according to basophil responsiveness to antigen. Mean maximum percent histamine release was 39,34 and 22%, respectively, for venom (100 ng/ ml), anti-IgE (0.25 µg/ml) and FMP (10^––⁴M). The amount of histamine specifically released by venom correlated significantly with anti-IgE but not with FMP-induced histamine release. Leukocytes were unresponsive to antigen in 10 subjects. The clinical characteristics and anaphylactic symptoms of these patients were not different from those with antigen-responsive cells. Unresponsive leukocytes responded to FMP in all and to anti-IgE in 8 of the 10 subjects. Mean anti-IgE and FMP-induced histamine release were, respectively, lower and higher than those observed with leukocytes responsive to antigen (p < 0.05). In unresponsive basophils, there was a negative correlation between maximum percent anti-IgE and FMP-induced histamine release. We confirm that basophils of a minority of the subjects allergic to Vespula venom do not release histamine in the presence of antigen. The negative correlation between anti-IgE and FMP-induced histamine release in unresponsive basophils may suggest individual differences in the ratio of FcΕRI and FMP receptors on the surface of basophils.

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Section: Leukocyte Releasabilitymentioning

confidence: 99%

Basophil Releasability in Patients with Hymenoptera Venom Allergy

Radermecker

Leclercq

Mariz³

et al. 1993

Int Arch Allergy Immunol

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“…It is now increasingly recognized that, besides these classical clinical properties, theophylline and more selective PDE inhibitors have anti-inflammatory and immunomodulatory effects. Indeed several studies have demonstrated that, in vitro, xanthines have a nonspecific suppressor activity in human peripheral lymphocytes [1], inhibit eosinophil degranulation [2], inhibit histamine release from mast cells and basophils [3], and have a direct inhibitory action on human alveolar respiratory burst [4]. These anti-inflammatory and immunomodulatory effects of xanthines have also been observed in vivo in animal models and also in humans.…”

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confidence: 97%

Theophylline and phosphodiesterase inhibitors

Aubier¹,

Barnes²

1995

Eur Respir J

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“…Whilst traditionally theophylline has been regarded as a bronchodilator, there is now growing evidence to suggest it possesses anti-inflammatory properties even at subtherapeutic levels [9±11]. Indeed, several studies have demonstrated that xanthines may exert their anti-inflammatory and immunomodulatory effects by the inhibition of eosinophil degranulation [12], histamine release from mast cells and basophils [13] antigen-and mitogen-induced T-cell proliferation [14] and T-cell recruitment into the bronchial mucosa [10], and the generation of cytokines [15]. However, the current knowledge concerning its anti-inflammatory mechanisms remains nondefinitive.…”

mentioning

confidence: 99%

Effects of theophylline, dexamethasone and salbutamol on cytokine gene expression in human peripheral blood CD4+ T-cells

Choy¹,

Ko²,

Li³

et al. 1999

Eur Respir J

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CD4+ T-cells are considered as pivotal in orchestrating the airway inflammation in asthma through the actions of their cytokines. Current hypothesis suggests that the anti-asthma effect of theophylline may be due to its anti-inflammatory actions, although the exact mechanisms remain unclear.The in vitro effect of theophylline on cytokine gene expression in peripheral blood CD4+ T-cells in normal subjects was compared with that of dexamethasone and salbutamol. CD4+ T-cells were cultured with phytohaemagglutin and phorbol myristate acetate in the presence of different concentrations of theophylline (10 -8 ±10 -3 M or 0.0018±180 mg . mL -1 ) in one group of subjects (n=8), dexamethasone (10 -9 ±10 -6 M or 0.39±390 ng . mL -1 ) in a second group (n=8) and salbutamol (10 -9 ±10 -4 M or 0.00058±58 mg . mL -1 ) in a third group (n=8). Gene expression of interleukin (IL)-3, IL-4, IL-5, granulocyte-macrophage colony-stimulating factor (GM-CSF) and interferon (IFN)-c was semiquantified by reverse transcription-polymerase chain reaction.Suppressed expression of IL-3 (36.9%), IL-4 (38.8%), GM-CSF (24.6%) and IFN-c (37.7%), but not of IL-5, was only seen with theophylline at a concentration of 10 -3 M (180 mg . mL -1 ) (p<0.05) and not at lower concentrations. In contrast, dexamethasone caused a dose-dependent suppression of transcription of all cytokines, with 39.5% for IL-3, 84.4% for IL-4, 40.6% for IL-5, 50.9% for GM-CSF and 31.8% for IFN-c at 10 -6 M (390 ng . mL -1 ) (p<0.05±0.001). Salbutamol did not suppress gene expression of any of the cytokines at the concentrations examined.These data suggest that cytokine gene expression of CD4+ T-cells is not affected at therapeutic concentrations of theophylline and salbutamol, but its suppression is likely to be an important mechanism underlying the therapeutic effect of corticosteroids in asthma.

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Substance P-Induced Histamine Release from Human Basophils, Skin and Lung Fragments: Effect of Nedocromil Sodium and Theophylline

Cited by 34 publications

References 14 publications

Basophil Releasability in Patients with Hymenoptera Venom Allergy

Basophil Releasability in Patients with Hymenoptera Venom Allergy

Theophylline and phosphodiesterase inhibitors

Effects of theophylline, dexamethasone and salbutamol on cytokine gene expression in human peripheral blood CD4+ T-cells

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