Substantial decrease of heat-shock protein 90 precedes the decline of sperm motility during cooling of boar spermatozoa

Huang, San-Yuan; Kuo, Y. H.; Lee, W.C; Tsou, H. L.; Lee, Y.P; Chang, Haixin; Wu, Jiong; Yang, Ping-Cheng

doi:10.1016/s0093-691x(99)00046-1

Cited by 67 publications

(38 citation statements)

References 31 publications

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“…The higher levels of HSP90AA1 in frozenthawed spermatozoa in our study may be a cell stress response to low temperature. However, other studies have shown that the levels of HSP90AA1 in spermatozoa are decreased substantially after cryopreservation (Huang et al 1999, Cao et al 2003a, 2003b. The relationship between cryopreservation and higher HSP90AA1 levels in boar sperm should be studied further.…”

Section: Sperm Energy Metabolismmentioning

confidence: 96%

“…It has been shown that the most evident damages are to the plasma membrane, acrosome, mitochondrial sheath (midpiece), and axonema after freezing and thawing (Cerolini et al 2001), and studies on the proteins of cryopreserved sperm have revealed profound implications for fertility and embryo development (Oliva et al 2009). Cryopreservation changes the functional state of many proteins, such as enzymes related to sperm metabolism (Huang et al 1999), proteins related to capacitation and acrosome reaction (Tabuchi et al 2008), proteins related to membrane and structure (Desrosiers et al 2006), and proteins related to apoptosis (Jeong et al 2009). All these variations influence the structural integrity, biological processes, and function of sperm, ultimately reducing their fertilization capacity.…”

Section: Introductionmentioning

confidence: 99%

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Identification of differentially expressed proteins in fresh and frozen–thawed boar spermatozoa by iTRAQ-coupled 2D LC–MS/MS

Chen

Zhu²,

Hu³

et al. 2014

Reproduction

101

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Cryodamage is a major problem in semen cryopreservation, causing changes in the levels of proteins that influence the function and motility of spermatozoa. In this study, protein samples prepared from fresh and frozen-thawed boar spermatozoa were compared using the isobaric tags for relative and absolute quantification (iTRAQ) labeling technique coupled to 2D LC-MS/MS analysis. A total of 41 differentially expressed proteins were identified and quantified, including 35 proteins that were present at higher levels and six proteins that were present at lower levels in frozen-thawed spermatozoa by at least a mean of 1.79-fold (P!0.05). On classifying into ten distinct categories using bioinformatic analysis, most of the 41 differentially expressed proteins were found to be closely relevant to sperm premature capacitation, adhesions, energy supply, and sperm-oocyte binding and fusion. The expression of four of these proteins, SOD1, TPI1, ODF2, and AKAP3, was verified by western blot analysis. We propose that alterations in these identified proteins affect the quality of cryopreserved semen and ultimately lower its fertilizing capacity. This is the first study to compare protein levels in fresh and frozenthawed spermatozoa using the iTRAQ technology. Our preliminary results provide an overview of the molecular mechanisms of cryodamage in frozen-thawed spermatozoa and theoretical guidance to improve the cryopreservation of boar semen.

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Section: Sperm Energy Metabolismmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Identification of differentially expressed proteins in fresh and frozen–thawed boar spermatozoa by iTRAQ-coupled 2D LC–MS/MS

Chen

Zhu²,

Hu³

et al. 2014

Reproduction

101

View full text Add to dashboard Cite

show abstract

“…The process of freezing-thawing could cause damage to the function of sperm proteins (Cao et al 2003;Martin et al 2007). Previous study suggested that some proteins also play important roles in the maintenance of sperm motility during the process of freezing-thawing (Huang et al 1999). Therefore, analyzing the change of protein in sperm could be a useful method to predict sperm quality.…”

Section: Introductionmentioning

confidence: 99%

Association of heat shock protein 70 with motility of frozen-thawed sperm in bulls

Zhang¹,

Hong²,

Yan³

et al. 2015

Czech J. Anim. Sci.

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ABSTRACT:Heat shock protein 70 (HSP70) is considered as a gene which affects semen quality traits. The present study attempted to investigate the relationship between the HSP70 expression level and motility of bull sperm during the process of freezing-thawing. Semen samples were collected from 5 QinChuan bulls by artificial vagina. Sperm motility and plasma membrane integrity of the semen samples at three stages (fresh, after equilibration, and frozen-thawed) were evaluated. The HSP70 expression level at the three stages was detected using real-time PCR. The results indicated that HSP70 expression level, membrane integrity, and sperm motility in the fresh semen were higher than those of the sperm after equilibration and freezing-thawing (P < 0.05), the HSP70 expression level, plasma membrane integrity, and sperm motility in sperm after equilibration were higher than those of the frozenthawed sperm (P < 0.05). The correlation between HSP70 expression level and sperm motility was positive (ranging from 0.327 to 0.785). The results suggest that HSP70 expression level in bull spermatozoa was gradually decreased following the process of freezing-thawing, and might be associated with bull sperm motility.

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“…In human (Cao et al, 2003) and boar (Huang et al, 1999), levels of heat-shock protein 90 (HSP 90) have been studied. The results demonstrated that HSP 90 decrease substantially in boar sperm after cryopreservation when analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blot (Huang et al, 1999). Cao et al (2003) found significantly decreased levels of HSP 90 after cryopreservation of human sperm.…”

Section: The Effect Of Cryopreservation On Spermatozoamentioning

confidence: 99%

The effect of cryopreservation on DNA damage, gene expression and protein abundance in vertebrate

Lin

Tsai

2012

Italian Journal of Animal Science

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Cryopreservation techniques allow the longterm storage of a wide variety of biological materials without significant deterioration in quality. Immediate post-thaw survival is most often used to assess the effect of the freezethaw process on cells. However, this parameter provides no information on possible subtle effects of cryopreservation, including DNA damage, and alteration of mRNA levels and protein function that may not be evident immediately post thaw. These potential adverse effects do not necessarily result in cell death. While there are many comprehensive reviews of gamete and embryo cryopreservation in vertebrate species, we review here the publications relating to the impact of cryopreservation on the genome of sperm, embryos and oocytes.

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Substantial decrease of heat-shock protein 90 precedes the decline of sperm motility during cooling of boar spermatozoa

Cited by 67 publications

References 31 publications

Identification of differentially expressed proteins in fresh and frozen–thawed boar spermatozoa by iTRAQ-coupled 2D LC–MS/MS

Identification of differentially expressed proteins in fresh and frozen–thawed boar spermatozoa by iTRAQ-coupled 2D LC–MS/MS

Association of heat shock protein 70 with motility of frozen-thawed sperm in bulls

The effect of cryopreservation on DNA damage, gene expression and protein abundance in vertebrate

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