Substituting fishmeal with Hermetia illucens in the diets of African catfish (Clarias gariepinus): Effects on growth, nutrient utilization, haemato-physiological response, and oxidative stress biomarker

Fawole, Femi John; Adeoye, Ayodeji A.; Tiamiyu, Lateef Oloyede; Ajala, Khadijah I.; Obadara, Saudat O.; Ganiyu, Ibrahim O.

doi:10.1016/j.aquaculture.2019.734849

Cited by 128 publications

(82 citation statements)

References 37 publications

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“…In the present study, the higher T-SOD and CAT activities observed in HILM-fed groups could be due to the accumulation of hydrogen peroxide (H 2 O 2 ), thereby triggering the activity of catalase enzyme to decompose H 2 O 2 and resulting in higher induction of CAT in HILM groups compared with the control. Our observation is in line with the opinion of Li et al (2017) and Fawole et al (2020) who found that CAT activities in fish increased when including HILM in their diets. Similarly, Xu et al (2020) showed that increasing HILM dietary content significantly increased plasma CAT activity and decreased MDA content of the juvenile mirror carp.…”

Section: Discussionsupporting

confidence: 93%

Effects of different levels of Hermetia illucens larvae meal on performance, egg quality, yolk fatty acid composition and oxidative status of laying hens

Liu

Yao

et al. 2021

Italian Journal of Animal Science

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To investigate the supplemental effects of Hermetia illucens larvae meal (HILM) on the production, egg quality, yolk fatty acid composition, egg amino acid content, oxidative status, and immune indexes of laying hens. A total of 432 Xuefeng black-bone chickens (45-week-old) were randomly assigned to 4 treatments with 6 replicates per treatment and 18 hens in each replicate. The laying hens were fed the basal diet supplemented with 0%, 1%, 3%, and 5% HILM (CON, HILM-1, HILM-3, and HILM-5, respectively) for 56 d. The experimental diets were isonitrogenous and isoenergetic. The highest egg weight and Haugh unit were obtained with 3% HILM supplementation, but the HILM-3 or HILM-5 supplementation have negative effect on the eggshell thickness, yolk index, and albumen height during the early stage of the experiment. The HILM supplementation linearly or quadratically (p < .05) increased the C14:0, C17:0, C18:2n6c, C20:2 contents of yolk, the Glu, Val, Met, Phe, and Leu contents of egg, the T-SOD and CAT activities, and the NDV-Ab and AIV-Ab levels of plasma. With increasing supplementation of HILM in the basal diet, FCR, the C16:0 and C16:1 contents of yolk, MDA content and IL-2 level of plasma decreased both linearly or quadratically (p < .05). In conclusion, Hermetia illucens larvae meal can be a suitable alternative protein source for Xuefeng black-bone chicken. Dietary supplementation of 3% HILM in basal diets may be a feasible means of effectively increasing the production performance of laying hens, and partially enhanced hens' antioxidant capability and immune function. HIGHLIGHTS Supplementation of 1% HILM had no effect on laying hens, and supplementation of 3% HILM increased the egg weight and Haugh unit. Supplementation of 3% HILM or 5% HILM have a negative effect on the eggshell thickness, yolk index, and albumen height during the early stage of the experiment. Supplementation of 3% HILM increased the C14:0, C17:0, and C20:2 contents of yolk, the Glu, Val, Met, Phe, and Leu contents of egg. The plasma antioxidant status and immune response improved by dietary addition of HILM at 3% levels.

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Section: Discussionsupporting

confidence: 93%

Effects of different levels of Hermetia illucens larvae meal on performance, egg quality, yolk fatty acid composition and oxidative status of laying hens

Liu

Yao

et al. 2021

Italian Journal of Animal Science

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“…In light of a sustainable circular economy, insects represent a very promising example of bio-converting organisms 7,9,19 . The substitution of dietary FM with BSF prepupae meal in aquafeeds is still controversial possibly due to differences in the diet formulation, the insects' biomass nutritional quality and the fish species used during the trials 7,10,11 . Growth substrate selection plays a key role on the nutritional quality of BSF biomass 32 and several studies have already demonstrated that BSF prepupae biomass can be improved by using an organic substrate containing proper amounts of PUFAs 6 .…”

Section: Discussionmentioning

confidence: 99%

Zebrafish (Danio rerio) physiological and behavioural responses to insect-based diets: a multidisciplinary approach

Zarantoniello

Randazzo

Gioacchini

et al. 2020

Sci Rep

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Black Soldier Fly (BSF) meal is considered as an alternative, emerging and sustainable ingredient for aquafeed production. However, results on fish physiological responses are still fragmentary and often controversial, while no studies are available on fish behavior in response to these new diets. The present work represents the first comprehensive multidisciplinary study aimed to investigate zebrafish physiological and behavioural responses to BSF-based diets. Five experimental diets characterized by increasing inclusion levels (0, 25, 50, 75 and 100% respect to fish meal) of full fat BSF prepupae meal were tested during a 2-months feeding trial. Prepupae were cultured on coffee silverskin growth substrate enriched with a 10% Schizochytrium sp. to improve insects' fatty acids profile. The responses of zebrafish were assayed through biometric, histological, gas chromatographic, microbiological, spectroscopic, molecular and behavioural analyses. Results evidenced that BSF-based diets affected fish fatty acid composition, while behavioural tests did not show differences among groups. Specifically, a 50% BSF inclusion level diet represented the best compromise between ingredient sustainability and proper fish growth and welfare. Fish fed with higher BSF inclusions (75 and 100%) showed hepatic steatosis, microbiota modification, higher lipid content, fatty acid modification and higher expression of immune response markers. The promotion of high-quality fish production as well as fish welfare are the main aquaculture goals, both strictly related to an adequate fish nutrition 1. The use of fish meal (FM) and fish oil (FO) in aquafeed formulation is no more feasible because of important environmental and economic issues 2. Over the last decades, several alternative ingredients (plant origin proteins, microalgae and processed animal proteins) to FM and FO have been tested 3. However, each of these ingredients showed some disadvantages in its application in aquafeed formulation including unbalanced amino acid profile, poor protein digestibility, presence of anti-nutritional factors and high production costs 3,4. Insects are now considered as an alternative and sustainable ingredient for feed production 5. In particular, the Black Soldier Fly (Hermetia illucens; BSF) larvae are one of the most promising candidates because of their proper protein content and the amino acid composition similar to that of FM 6,7. In addition, BSF have low environmental requirements, a high feed conversion efficiency and they can growth on organic by-products, promoting sustainability and the circular economy concept in the aquaculture sector 8,9. Several studies tested different BSF inclusion levels in aquafeed formulation but results on fish physiological responses are still controversial, while behavioural effects on fish are completely missing 10-13. The use of BSF in aquafeed has been shown, at certain inclusion levels,

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“…Due to a favourable nutritional profile, compatible with the nutritional requirement of most aquaculture species 1 , the aquaculture industry has traditionally relied on fishmeal (FM) as the main protein source 2 . Since this dietary protein source is considered environmentally and ecologically unsustainable, there is societal and economical pressure on the aquaculture industry to search for alternative protein ingredients.…”

Section: Introductionmentioning

confidence: 99%

Supplementation of tuna hydrolysate and insect larvae improves fishmeal replacement efficacy of poultry by-product in Lates calcarifer (Bloch, 1790) juveniles

Chaklader

Howieson

Siddik

et al. 2021

Sci Rep

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The effects of feeding different levels of poultry by-product meal (PBM) replacing fishmeal (FM) protein, supplemented with tuna hydrolysate (TH) and Hermetia illucens (HI) larvae, on the growth, fillet quality, histological traits, immune status, oxidative biomarker levels and gut microbiota of juvenile barramundi, Lates calcarifer were investigated for six weeks. Barramundi were fed four isonitrogenous and isolipidic diets in which a FM based diet was used as the Control diet (Diet1) and compared with other non-FM diets containing 80%, 85% and 90% PBM along with the concurrent supplementation of 5% and/or 10% TH and HI larvae meal. These treatment diets were designated as 80PBM10TH+10HI (Diet2), 85PBM5TH+10HI (Diet3) and 90PBM5TH+5HI (Diet4). The growth and condition factor of fish fed 80PBM10TH+10HI and 85PBM5TH+10HI were significantly higher than the Control. Total saturated, monounsaturated and polyunsaturated fatty acid retention in the fish muscle increased in fish fed PBM-based diets, supplemented with TH and HI larvae meal, with no adverse effect on post-harvest characteristics such as texture and colour of fish fillets. Improvement in serum total bilirubin and total protein content was found in all fish fed TH and HI larvae supplemented PBM. Similarly, immune response showed a significant increase in fish fed non-FM test diets than the Control. In the distal intestine, supplementation of any quantities of TH and HI larvae to PBM led to an increase in the microvilli density and neutral mucins while the number of goblet cells in the skin were unchanged. Liver, kidney, and spleen histology demonstrated a normal structure with no obvious changes in response to all test diets. Bacterial diversity increased in fish fed Diets 2 and 3 with a high abundance of Proteobacteria in Diets 1 and 4 and Firmicutes in Diets 2 and 3. The fish on test diets showed a lower abundance of genus Vibrio. Fish fed TH and HI larvae supplemented PBM diets showed lower infection rate to V. harveyi than the Control. Collectively, concurrent supplementation of TH and HI larvae could improve the quality of PBM diets with positive effects on growth, fillet quality, intestinal health, immunity, and disease resistance.

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Substituting fishmeal with Hermetia illucens in the diets of African catfish (Clarias gariepinus): Effects on growth, nutrient utilization, haemato-physiological response, and oxidative stress biomarker

Cited by 128 publications

References 37 publications

Effects of different levels of Hermetia illucens larvae meal on performance, egg quality, yolk fatty acid composition and oxidative status of laying hens

Effects of different levels of Hermetia illucens larvae meal on performance, egg quality, yolk fatty acid composition and oxidative status of laying hens

Zebrafish (Danio rerio) physiological and behavioural responses to insect-based diets: a multidisciplinary approach

Supplementation of tuna hydrolysate and insect larvae improves fishmeal replacement efficacy of poultry by-product in Lates calcarifer (Bloch, 1790) juveniles

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