Substitution mutation induced migration anomaly of a D10S2325 allele on capillary electrophoresis

Chen, Pengyu; Zhang, Ji; Luo, Haibo; Schneider, Peter M.; Hou, Yiping

doi:10.1007/s00414-012-0779-2

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…Forensic geneticists and forensic DNA analysts are extremely strict about the accuracy and precision of the genotyping results produced by STR kits in their daily work. Even if the size of STR-PCR products differs by only one base, it is possible to have a serious effect on genotyping, resulting in incorrect typing results ( Chen et al, 2013 ) which may even lead to the wrongful arrest or release of criminal suspects. In practical work, due to the need of case investigation, the STR-PCR products are usually detected at different time points several times to ensure the accuracy and reliability of the typing results.…”

Section: Discussionmentioning

confidence: 99%

Excessive addition split peak formed by the non-templated nucleotide addition property of Taq DNA polymerase after PCR amplification

Zhou

Fan

Tian

et al. 2023

Front. Bioeng. Biotechnol.

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Because of its non-template addition feature, Taq DNA polymerase can catalyze one or more extra nucleotides onto the 3′ terminus of PCR products. An extra peak is observed at DYS391 locus after the PCR products stored for 4 days at 4°C. To explore the formation mechanism of this artifact, PCR primers and amplicon sequences of Y-STR loci are analyzed, furthermore, PCR products storage conditions and termination of PCR are discussed. The extra peak is a + 2 addition product, which we call excessive addition split peak (EASP). The most significant difference between EASP and the incomplete addition of adenine product is that the size of EASP is about one base larger than the true allele, and the EASP locates on the right side of the real allelic peak. The EASP cannot be eliminated by increasing loading mixture volume and conducting heat denaturation prior to electrophoresis injection. However, the EASP is not observed when the PCR is terminated with ethylenediaminetetraacetic acid or formamide. These findings suggest that formation of EASP is a result of 3′ end non-template extension by Taq DNA polymerase, rather than being the result of DNA fragment secondary structure produced under a suboptimal electrophoresis condition. In addition, the EASP formation is affected by the primer sequences and the storage conditions of PCR products.

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Section: Discussionmentioning

confidence: 99%

Excessive addition split peak formed by the non-templated nucleotide addition property of Taq DNA polymerase after PCR amplification

Zhou

Fan

Tian

et al. 2023

Front. Bioeng. Biotechnol.

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“…Evident differences (P < 0.05) were found between the Hunan Han and the Dutch [10], North Italian [11], Somali [12], Lithuanian (except D3S1744, P = 0.0723) [13] and Swedish (except D3S1744, P = 0.1267 and D8S1132, P = 0.2776) [12] populations. Significant differences were also observed from the Hunan Han population at D2S1360, D3S1744, D10S2325, SE33 and D12S391 with the Hebei Han [4]; at D4S2366, D8S1132, SE33 and D12S391 with the Sichuan Han [14]; and at D3S1744, D10S2325, SE33 and D12S391 with the Shanghai Han [15]. Table S4 shows the genetic distances between Hunan Han and other populations.…”

Section: Dear Editormentioning

confidence: 92%

Genetic polymorphism of 29 STR loci in the Hunan Han population from China

Liu

Guo

et al. 2017

Forensic Sciences Research

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Genetic polymorphism of the 26 short tandem repeat loci in the Chinese Hebei Han population using two commercial forensic kits

Liu

et al. 2014

Mol Biol Rep

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We determined the allele frequencies and forensic parameters for the 26 short tandem repeat (STR) autosomal markers in two commercial kits (the Investigator HDplex and AmpFLSTR(®) Identifiler(®) systems) for 183 unrelated individuals from the Han population of the Hebei Province of China. The 26 STRs were all in Hardy-Weinberg equilibrium. No linkage disequilibrium was detected between any pair of loci. The combined power of discrimination and the combined power of exclusion for the 26 STR loci were 1-7.74E-31 and 1-1.21E-11, respectively. Six rare alleles of D10S2325 were identified and named 20, 21, 22, 23, 24, and 31. All the length of the six rare alleles were out of the range of allelic ladder. We calculated the population pairwise genetic distance based on the allele frequencies, using published population data including German, central Polish, south Dutch, northeastern Polish, south Brazilian, Korean, Sichuan Han of China, and Shanghai Han of China. Also we examined the population pairwise genetic distance of loci included in Identifiler system between Hebei Han and other ethnic population of China. These 26 autosomal STR loci could provide highly informative polymorphic data for paternity testing and forensic identification in the Hebei Han population in China. Because they are all in linkage equilibrium, they could be used together to solve deficient kinship cases or cases with mutations.

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Substitution mutation induced migration anomaly of a D10S2325 allele on capillary electrophoresis

Cited by 3 publications

References 26 publications

Excessive addition split peak formed by the non-templated nucleotide addition property of Taq DNA polymerase after PCR amplification

Excessive addition split peak formed by the non-templated nucleotide addition property of Taq DNA polymerase after PCR amplification

Genetic polymorphism of 29 STR loci in the Hunan Han population from China

Genetic polymorphism of the 26 short tandem repeat loci in the Chinese Hebei Han population using two commercial forensic kits

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