Substrate conductivity dependent modulation of cell proliferation and differentiation in vitro

Thrivikraman, Greeshma; Mallik, Prafulla Kumar; Basu, Bikramjit

doi:10.1016/j.biomaterials.2013.05.076

Cited by 80 publications

(56 citation statements)

References 30 publications

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“…The lengths of myotubes were typically 1.7–3.2 folds higher on the HPLAAT than on HPLA, which demonstrated again the positive correlation between the increase of myotube length and AT contents. These results were also consistent with other reports about myogenic differentiation on conductive substrates [6, 9, 41, 42]. The diameters of myotubes were also measured to further quantify myotube formation on these polymer substrates.…”

Section: Resultssupporting

confidence: 90%

“…Conducting scaffolds are attractive for skeletal muscle tissue engineering because they not only provide physical support, but also transmit electrical signal [2, 4–7]. Conducting substrates derived from polypyrrole (PPy) [8], HA-CaTiO3 (hydroxyapatite-calcium titanate), [9], polyaniline (PANi) and poly(ε-caprolactone) (PCL) [6] show positive effect in promoting the proliferation, differentiation, and maturation of skeletal muscle tissue in comparison with non-conductive polymeric substrates [2]. However, there are significant challenges in utilizing these conductive materials for muscle regeneration because of their low or non-degradability, poor mechanical properties, poor solubility and poor processability [10–15].…”

Section: Introductionmentioning

confidence: 99%

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Ductile electroactive biodegradable hyperbranched polylactide copolymers enhancing myoblast differentiation

et al. 2015

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Myotube formation is crucial to restoring muscular functions, and biomaterials that enhance the myoblast differentiation into myotubes are highly desirable for muscular repair. Here, we report the synthesis of electroactive, ductile, and degradable copolymers and their application in enhancing the differentiation of myoblasts to myotubes. A hyperbranched ductile polylactide (HPLA) was synthesized and then copolymerized with aniline tetramer (AT) to produce a series of electroactive, ductile and degradable copolymers (HPLAAT). The HPLA and HPLAAT showed excellent ductility with strain to failure from 158.9% to 42.7% and modulus from 265.2 to 758.2 MPa. The high electroactivity of the HPLAAT was confirmed by UV spectrometer and cyclic voltammogram measurements. These HPLAAT polymers also showed improved thermal stability and controlled biodegradation rate compared to HPLA. Importantly, when applying these polymers for myotube formation, the HPLAAT significantly improved the proliferation of C2C12 myoblasts in vitro compared to HPLA. Furthermore, these polymers greatly promoted myogenic differentiation of C2C12 cells as measured by quantitative analysis of myotube number, length, diameter, maturation index, and gene expression of MyoD and TNNT. Together, our study shows that these electroactive, ductile and degradable HPLAAT copolymers represent significantly improved biomaterials for muscle tissue engineering compared to HPLA.

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Section: Resultssupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Ductile electroactive biodegradable hyperbranched polylactide copolymers enhancing myoblast differentiation

et al. 2015

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“…However, coating the substrates with conductive films of gold and titanium did upregulate expression of these markers. The authors attribute this to the changes in inherent conductivity – a property known to upregulate myogenesis as reported elsewhere384647. It is possible that the regulatory effects of conductivity on differentiation are seen herein.…”

Section: Discussion/conclusionmentioning

confidence: 55%

Relationship between nanotopographical alignment and stem cell fate with live imaging and shape analysis

Newman

Galenano-Niño

Graney

et al. 2016

Sci Rep

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The topography of a biomaterial regulates cellular interactions and determine stem cell fate. A complete understanding of how topographical properties affect cell behavior will allow the rational design of material surfaces that elicit specified biological functions once placed in the body. To this end, we fabricate substrates with aligned or randomly organized fibrous nanostructured topographies. Culturing adipose-derived stem cells (ASCs), we explore the dynamic relationship between the alignment of topography, cell shape and cell differentiation to osteogenic and myogenic lineages. We show aligned topographies differentiate cells towards a satellite cell muscle progenitor state - a distinct cell myogenic lineage responsible for postnatal growth and repair of muscle. We analyze cell shape between the different topographies, using fluorescent time-lapse imaging over 21 days. In contrast to previous work, this allows the direct measurement of cell shape at a given time rather than defining the morphology of the underlying topography and neglecting cell shape. We report quantitative metrics of the time-based morphological behaviors of cell shape in response to differing topographies. This analysis offers insights into the relationship between topography, cell shape and cell differentiation. Cells differentiating towards a myogenic fate on aligned topographies adopt a characteristic elongated shape as well as the alignment of cells.

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“…Importantly, a higher current (40 mA) could increase the surface extracellular substances and the net negative surface electrostatic charge. Further, the manipulation of cell behavior in the presence of electric field depends on the size, shape, membrane thickness of the cells, strength and duration of applied electric field [14][15][16]. It is reported that the weak electric fields enhance the efficacy of antibiotics in killing bacterial biofilm even on the surfaces that are not used as electrodes [17].…”

Section: Introductionmentioning

confidence: 99%

“…As far as the sole influence of substrate conductivity effect is concerned, an earlier study from our group illustrated the role of substrate conductivity as a guiding factor in the orientation and differentiation of C2C12 mouse myoblast cells on moderately conducting HACaTiO 3 substrates in the absence of electric field stimulation during culture [16]. In a follow-up study, our research group also reported the influence of pulse electrical stimulation (lateral electric field) on the osteogeneic cell proliferation on HACaTiO 3 substrates [34] and stainless steel [35] over a narrow window of field strength.…”

mentioning

confidence: 99%

Vertical electric field induced bacterial growth inactivation on amorphous carbon electrodes

Jain

Sharma

Basu

2015

Carbon

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Substrate conductivity dependent modulation of cell proliferation and differentiation in vitro

Cited by 80 publications

References 30 publications

Ductile electroactive biodegradable hyperbranched polylactide copolymers enhancing myoblast differentiation

Ductile electroactive biodegradable hyperbranched polylactide copolymers enhancing myoblast differentiation

Relationship between nanotopographical alignment and stem cell fate with live imaging and shape analysis

Vertical electric field induced bacterial growth inactivation on amorphous carbon electrodes

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