Success rates in isolating mesenchymal stem cells from permanent and deciduous teeth

Nakajima, Kengo; Kunimatsu, Ryo; Ando, Kazuyo; Hiraki, Tomoka; Rikitake, Kodai; Tsuka, Yuji; Abe, Takaharu; Tanimoto, Kotaro

doi:10.1038/s41598-019-53265-4

Cited by 17 publications

(16 citation statements)

References 29 publications

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“…For instance, Biebak et al [ 28 ] optimized the protocols to increase the isolation yield of umbilical cord blood MSCs (UCB-MSCs) up to 63%, while BMSCs were used as a positive control (success rate 100%). Nakajima et al [ 22 ] investigated the success rate of isolating DPSCs and SHED, reporting 70% and 82%, respectively. Neither of these studies harvested the two cell types from the same donor, most probably owing to the apparent difficulty in collecting cells from two different sites within the same patient, and hence derives the relevance of the protocol proposed in the present work.…”

Section: Discussionmentioning

confidence: 99%

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Isolation and Characterization of Buccal Fat Pad and Dental Pulp MSCs from the Same Donor

et al. 2021

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Mesenchymal stem cells (MSCs) can be harvested from different sites in the oral cavity, representing a reservoir of cells useful for regenerative purposes. As direct comparisons between at least two types of MSCs deriving from the same patient are surprisingly rare in scientific literature, we isolated and investigated the osteoinductive potential of dental pulp stem cells (DPSCs) and buccal fat pad stem cells (BFPSCs). MSCs were isolated from the third molar dental pulp and buccal fat pads of 12 patients. The number of viable cells was quantified through manual count. Proliferation and osteodifferentiation assays, flow cytometry analysis of cell phenotypes, and osteocalcin release in vitro were performed. The isolation of BFPSCs and DPSCs was successful in 7 out of 12 (58%) and 3 out of 12 (25%) of retrieved samples, respectively. The yield of cells expressing typical stem cell markers and the level of proliferation were higher in BFPSCs than in DPSCs. Both BFP-SCs and DPSCs differentiated into osteoblast-like cells and were able to release a mineralized matrix. The release of osteocalcin, albeit greater for BFPSCs, did not show any significant difference between BFPSCs and DPSCs. The yield of MSCs depends on their site of origin as well as on the protocol adopted for their isolation. Our data show that BFP is a valuable source for the derivation of MSCs that can be used for regenerative treatments.

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Section: Discussionmentioning

confidence: 99%

“…According to the protocol described by Nakajima et al [ 22 ], successful isolation was defined when the cell culture reached the third passage (P3) without any contamination. Cells were observed daily under an optical microscope until the day when cell adhesion was observed, and colonies were formed.…”

Section: Methodsmentioning

confidence: 99%

Isolation and Characterization of Buccal Fat Pad and Dental Pulp MSCs from the Same Donor

et al. 2021

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“…In agreement with 6). In addition, other related factors, such as the patient's age, length of the remaining root, and mechanical stress influence the successful isolation and establishment of SHEDs (17). SHEDs from both groups in established cultures displayed a similar, characteristic spindle-shaped, fibroblastlike morphology.…”

Section: Discussionmentioning

confidence: 99%

“…It was suggested that the pulp acquired from deciduous teeth without root resorption did not support the derivation of viable cell lines in comparison to those from teeth with resorbed roots (15). Although the detailed biological properties of SHED are being rigorously investigated (5,16,17), the effect of different levels of root resorption on the characteristics of cell lines obtained from these teeth remains inconclusive. Thus, the tooth source that is most suitable for the isolation of SHEDs needs to be determined.…”

Section: Dental Pulp Stem Cells (Dpscs) From Impacted Third Molarsmentioning

confidence: 99%

Comparison of stem cells from human exfoliated deciduous posterior teeth with varying levels of root resorption

et al. 2021

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Background: Stem cells from human exfoliated deciduous teeth (SHED) are regarded as an attractive cell source for tissue regeneration. However, the effect of different levels of root resorption on the characteristics of SHED remains less understood. Thus, the tooth source that is most suitable for the isolation of SHEDs needs to be determined. To compare cellular and biological characteristics of stem cells from human exfoliated deciduous posterior teeth with varying levels of root resorption. Methods:The pulp was obtained from the deciduous posterior teeth depending on the level of root resorption, and isolated SHEDs were grouped as follows: Teeth with 0 to 1/3 rd root resorption as SHEDs (G1) and 1/3 rd to 2/3 rd root resorption as SHEDs (G2). Teeth were also collected from >2/3 rd root resorption status, but failed to establish primary culture of SHED as the availability of pulp tissue was too less. Later, isolated SHEDs were compared on their morphology, viability, growth kinetics, colony-forming ability, expression of cell surface markers and in vitro differentiation into osteocytes and adipocytes.Results: No major differences were observed in terms of cellular morphology, viability, proliferation rate, colony-forming ability, cell surface markers expression, and mesenchymal lineage differentiation of SHEDs isolated from posterior teeth with 0 to 1/3 rd and 1/3 rd to 2/3 rd root resorption. However, SHED from teeth with 0 to 1/3 rd root resorption (G1) displayed relatively higher proliferation capacity and expression of selected markers.Conclusions: Collectively, SHEDs (G1) and SHEDs (G2) showed comparable cellular and biological characteristics that enable their possible applications in regenerative therapies.

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“…Human dental pulp stem cells and SHED are obtained non-invasively, reducing the physical burden on patients, and these cells have the same regenerative ability as BMSCs [17][18][19][20] . However, transplantation of BMSCs does not result in signi cantly higher bone regeneration compared to autogenous bone graft 21,22 .…”

Section: Discussionmentioning

confidence: 99%

Effect of CD146 on Bone Regeneration by Transplantation of Stem Cells from Human Exfoliated Deciduous Teeth into Mouse Skull Defect

Rikitake

Kunimatsu

Yoshimi

et al. 2021

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Stem cells from human exfoliated deciduous teeth (SHED) possess bone regeneration ability and may have therapeutic applications. CD146, a cell adhesion protein expressed by vascular endothelial cells, is involved in the osteoblastic differentiation of stem cells. However, the effect of CD146 on SHED-mediated bone regeneration in vivo remains unknown. Hence, in this study we aimed to establish efficient conditions for SHED transplantation. SHED were isolated from the pulp of an extracted deciduous tooth and cultured, and CD146-positive (CD146+) and CD146-negative (CD146−) populations were sorted. Heterogeneous populations of SHED and CD146+ and CD146– cells were transplanted into bone defects generated in the skulls of individual immunodeficient mice. Micro-computed tomography was performed immediately post-transplantation and at 4- and 8-weeks thereafter to evaluate bone regeneration. Histological and immunohistochemical assessments were also performed at 8 weeks after transplantation. Micro-computed tomography revealed bone regeneration upon transplantation with CD146+ and heterogeneous populations of SHED, particularly at 8 weeks after transplantation, with significantly higher bone regeneration observed following transplantation with CD146+ cells. Furthermore, histological and immunohistochemical assessments revealed that CD146+ cells promoted bone regeneration and angiogenesis. Therefore, transplantation of CD146+ SHED into bone defects may serve as a useful strategy for bone regeneration.

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Success rates in isolating mesenchymal stem cells from permanent and deciduous teeth

Cited by 17 publications

References 29 publications

Isolation and Characterization of Buccal Fat Pad and Dental Pulp MSCs from the Same Donor

Isolation and Characterization of Buccal Fat Pad and Dental Pulp MSCs from the Same Donor

Comparison of stem cells from human exfoliated deciduous posterior teeth with varying levels of root resorption

Effect of CD146 on Bone Regeneration by Transplantation of Stem Cells from Human Exfoliated Deciduous Teeth into Mouse Skull Defect

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