Successful pregnancy after intracytoplasmic sperm injection with testicular spermatozoa transported only under refrigeration

Yamashita²,

Tsujimoto³

et al. 2017

Clin Exp Reprod Med

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ObjectiveOocyte degeneration often occurs after intracytoplasmic sperm injection (ICSI), and the risk factor is low-quality oocytes. The follicular fluid (FF) provides a crucial microenvironment for oocyte development. We investigated the relationships between the FF volume aspirated from individual follicles and oocyte retrieval, oocyte maturity, oolemma stretchability, fertilization, and development.MethodsThis retrospective study included data obtained from 229 ICSI cycles. Ovarian stimulation was performed according to a gonadotropin-releasing hormone antagonist protocol. Each follicle was individually aspirated and divided into six groups according to FF volume (<1.0, 1.0 to <2.0, 2.0 to <3.0, 3.0 to <4.0, 4.0 to <5.0, and ≥5.0 mL). Oolemma stretchability during ICSI was evaluated using a mechanical stimulus for oolemma penetration, that is, the stretchability was assessed by oolemma penetration with aspiration (high stretchability) or without aspiration (low stretchability).ResultsOocyte retrieval rates were significantly lower in the <1.0 mL group than in the ≥1.0 mL groups (46.0% [86/187] vs. 67.5%–74.3% [172/255 to 124/167], respectively; p<0.01). Low oolemma stretchability was significantly more common in the <1.0 mL group than in the ≥1.0 mL groups during ICSI (22.0% [13/59] vs. 5.8%–9.4% [6/104 to 13/139], respectively; p=0.018). There was a relationship between FF volume and oolemma stretchability. However, there were no significant differences in the rates of fertilization, cleavage, ≥7 cells at day 3, and blastocyst development among all groups.ConclusionFF volume is potentially associated with the stretchability of metaphase II oolemma during ICSI. Regarding oolemma stretchability, ensuring a uniform follicular size during ovarian stimulation is crucial to obtain good-quality oocytes.

Section: Methodsmentioning

confidence: 99%

The association of follicular fluid volume with human oolemma stretchability during intracytoplasmic sperm injection

Yamashita²,

Tsujimoto³

et al. 2017

Clin Exp Reprod Med

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“…Controlled ovarian stimulation was performed using a long, short, or gonadotropin‐releasing hormone (GnRH) antagonist protocol depending on the patient as described previously. 16 , 17 , 18 A GnRH analogue acetate (Fuji Pharma Co., Tokyo, Japan), human menopausal gonadotropin (ASKA Pharmaceutical Co., Tokyo, Japan), and a GnRH antagonist (Cetrotide; Merck KGaA, Darmstadt, Germany) were administered as dictated in these established protocols. When at least 2 follicles reached 18–20 mm in diameter (as determined by transvaginal ultrasonography), 5000 IU of hCG (Fuji Pharma Co.) was administered.…”

Section: Methodsmentioning

confidence: 99%

The migration speed of nucleolar precursor bodies in pronuclei affects in vitro fertilization‐derived human embryo ploidy status and live birth

Reprod Medicine & Biology

Taguchi²,

Uemura

et al. 2023

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Purpose To study the relationship between clinical outcomes after assisted reproduction and the migration speed of nucleolus precursor bodies (NPBs) in male and female pronuclei (mPN; fPN). Methods NPB migration speed, embryo ploidy status, and live birth (LB) were retrospectively analyzed in IVF‐derived zygotes. The central coordinates of the mPN, fPN, and NPBs were noted at multiple timepoints. The migration distance of NPBs between two sequential images was measured to calculate NPB migration speed. Results The NPB migration speeds in mPN and fPN were significantly faster in euploid zygotes than in aneuploid zygotes. In multivariate logistic analysis, NPB migration speed in mPN and the female age were associated with euploidy. The NPB migration speeds in mPN and fPN were also significantly faster in zygotes that led to LB than in zygotes that led to no pregnancy. In a receiver operating characteristic curve analysis of LB by NPB migration speed in mPN, the cut‐off value was 3.74 μm/h (AUC: 0.825, 95%CI: 0.688–0.963). When the zygotes were categorized by this cut‐off value, there were significantly more LBs in zygotes with migration speed ≥ the cut‐off (78.9% vs. 21.1%). Conclusions Zygotes with quickly migrating NPBs demonstrated the developmental potential to become a baby.

“…Several spermatozoa were picked up, washed with a 5 μL drop of 5% polyvinylpyrrolidone (Irvine Scientific, Santa Ana, CA, USA) that was dissolved in mHTF with 10% serum protein substitute, and immobilized. The oocytes were inseminated through ICSI by using injection pipettes (inner diameter: 4.7 μm; outer diameter: 6.0 μm; Cook Medical, Bloomington, IN, USA) . The level of oolemma stretchability was evaluated, as described previously .…”

Section: Methodsmentioning

confidence: 99%

Association of follicular fluid volume with membrane stretchability of human metaphase II oocytes following the gonadotropin‐releasing hormone agonist protocol during intracytoplasmic sperm injection

Reprod Medicine & Biology

Taguchi²,

Hirao³

et al. 2018

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PurposeThe authors previously revealed the association of the follicular fluid (FF) volume with oolemma stretchability following the gonadotropin‐releasing hormone (GnRH) antagonist protocol during intracytoplasmic sperm injection (ICSI). However, the impact of the GnRH agonist protocol on oolemma stretchability remains unclear.MethodsData that were obtained from 74 ICSI cycles were reviewed retrospectively. Controlled ovarian stimulation was performed in accordance with the short GnRH agonist protocol. Each follicle was individually aspirated and assigned to one of six groups, according to the FF volume. The oolemma stretchability during ICSI was evaluated by using a mechanical stimulus for oolemma penetration; that is, oolemma penetration with or without aspiration (high vs low stretchability, respectively).ResultsThe incidence of low oolemma stretchability was significantly higher in the <1.0 mL group than that in the ≥1.0 mL group. The normal fertilization rate was significantly lower in the <1.0 mL group than that in the 2.0‐<3.0 mL group. The rate of blastocyst development was lower in the <1.0 mL group than that in the 3.0‐<4.0 mL group.ConclusionThe FF volume potentially was associated with metaphase II oolemma stretchability, fertilization, and blastocyst development.