Sucrose metabolism in sugarcane cell suspension cultures

Goldner, William; Thom, Margaret; Maretzki, Andrew

doi:10.1016/0168-9452(91)90021-y

Cited by 44 publications

(31 citation statements)

References 16 publications

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“…Sucrose is hydrolyzed (1%) when the culture medium is autoclaved. During culture, hexoses are also gradually hydrolyzed by invertases located in the cell wall or in the plasmalemma (Goldner et al 1991;Wendler et al 1991). In general, significant levels of free invertases were not detected in the culture medium, although this could sometimes occur (Goldner et al 1991;Wendler et al 1991).…”

Section: Discussionmentioning

confidence: 96%

CO2-enriched microenvironment affects sucrose and macronutrients absorption and promotes autotrophy in the in vitro culture of kiwi (Actinidia deliciosa Chev. Liang and Ferguson)

Arigita

Cañal

Tamés

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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In traditional in vitro culture, the low CO 2 concentration inside the vessels restricts photosynthesis and necessitates the addition of sucrose to the culture medium as the main energy source, thus bringing about changes in the absorption of mineral elements from the culture medium. In this study, we investigated macronutrient absorption and sugar consumption in Actinidia deliciosa Chevalier Liang and Ferguson cv. Hayward (kiwi), cultured on medium supplemented with varying amounts of sucrose (0, 10, and 20 g l −1 ) under both heterotrophy and autotrophy, flushed with different concentrations of CO 2 (non-ventilation, 300, 600, and 2,000 μl l −1 ). In ventilated systems with 20 g l −1 of sucrose, sucrose absorption was less than under non-ventilation. The lowest rate of sucrose absorption was recorded when the explants were cultured on medium supplemented with 20 g l −1 of sucrose and flushed with 600 μl l −1 CO 2 . Absorption of NO 3 − , PO 4 3− , and Mg 2+ were high (maximum) at the end of the culture period (40 d) in explants flushed with 600 μl l −1 CO 2 that have been cultured 20 d in the presence of sucrose and then transferred to a sucrose-free medium. These autotrophic conditions promoted maximum plant growth in terms of both fresh and dry mass as well as the length and number of shoots and leaves. The study shows that to maintain an optimum regime of mineral nutrition for prolonged culture of kiwi in vitro, an increased amount of these three ions should be supplemented in Murashige and Skoog's medium.

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Section: Discussionmentioning

confidence: 96%

CO2-enriched microenvironment affects sucrose and macronutrients absorption and promotes autotrophy in the in vitro culture of kiwi (Actinidia deliciosa Chev. Liang and Ferguson)

Arigita

Cañal

Tamés

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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“…Afterward, the two resulting hexoses are readily taken up by the cells. However, no important secretion of free invertase enzymes into the culture medium could be detected by several authors (Goldner et al, 1991;Wendler et al, 1991;Kanabus et al, 1986) although it might occur (King and Street, 1977). Also for the used culture system no apparent 14C-sucrose degradation could be detected when samples of media that were previously used for cultivation of rose microshoots were incubated.…”

Section: Mechanism Of Sucrose Hydrolysis and Absorptionmentioning

confidence: 93%

Sucrose uptake and metabolism in a double layer system for micropropagation ofRosa multiflora

Riek

Piqueras

Debergh

1997

Plant Cell Tiss Organ Cult

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Uptake and metabolism of sucrose in micropropagated Rosa multiflora using the double layer technique was investigated. In the multiplication as well as the root induction stage, hydrolysis of sucrose in the culture medium was observed. A mathematical model was developed to quantify sucrose hydrolysis and the uptake of sucrose, glucose and fructose, based on the time series for the different sugars in the culture medium. These data were linked to a study of the sugar metabolism in the microshoots. After 48 h of incubation on 14C-[U]-glucose containing medium, the incorporated label was mainly detected in the ethanol soluble fraction; within this fraction sucrose was the most important compound. This indicates a significant re-synthesis of sucrose in the plant material after the uptake of hexose. To assess the extent that different enzymes of sucrose metabolism (invertases, sucrose synthase and sucrose-P-synthase) were involved, their activity in different plant parts (of final stage III microshoots) were assayed. A decreasing gradient for sucrose metabolising enzymes from the roots toward the leaves gave a good indication of how the different tissues depend on sucrose absorbed from the medium.

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“…If Suc is predominantly synthesized through SUC phosphate synthase (Wendler et al, 1990;Goldner et al, 1991), then both the fructosyl and glucosyl moieties of the synthesized Suc molecule would be labeled at more or less the same frequency. In a11 the internodes analyzed after feeding with labeled Glc, significant amounts of label were found in the Fru moiety of SUC.…”

Section: Carbon Cyclingmentioning

confidence: 99%

Carbon Partitioning during Sucrose Accumulation in Sugarcane Internodal Tissue

1997

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l h e temporal relationship between sucrose (Suc) accumulation and carbon partitioning was investigated in developing sugarcane internodes. Radiolabeling studies on tissue slices, which contained Suc concentrations ranging from 14 to 42% of the dry mass, indicated that maturation coincided with a redirection of carbon from water-insoluble matter, respiration, amino acids, organic acids, and phosphorylated intermediates into SUC. It is evident that a cycle of Suc synthesis and degradation exists in all of the internodes. l h e decreased allocation of carbon to respiration coincides with a decreased flux from the hexose pool. 60th the glucose and fructose (Fru) concentrations significantly decrease during maturation. The phosphoenolpyruvate, Fru-6-phosphate (Fru-6-P), and Fru-2,6-bisphosphate (Fru-2, 6-P,) concentrations decrease between the young and older internodal tissue, whereas the inorganic phosphate concentration increases. lhe calculated mass-action ratios indicate that the ATP-dependent phosphofructokinase, pyruvate kinase, and Fru-l,6-bisphosphatase reactions are tightly regulated in all of the internodes, and no evidence was found that major changes in the regulation of any of these enzymes occur. l h e pyrophosphatedependent phosphofructokinase reaction is in apparent equilibrium in ali the internodes. Substrate availability might be one of the prime factors contributing to the observed decrease in respiration.

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Sucrose metabolism in sugarcane cell suspension cultures

Cited by 44 publications

References 16 publications

CO2-enriched microenvironment affects sucrose and macronutrients absorption and promotes autotrophy in the in vitro culture of kiwi (Actinidia deliciosa Chev. Liang and Ferguson)

CO2-enriched microenvironment affects sucrose and macronutrients absorption and promotes autotrophy in the in vitro culture of kiwi (Actinidia deliciosa Chev. Liang and Ferguson)

Sucrose uptake and metabolism in a double layer system for micropropagation ofRosa multiflora

Carbon Partitioning during Sucrose Accumulation in Sugarcane Internodal Tissue

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