Background: This field experiment investigated the acute effects of brief mindfulness-based intervention (MBI) coupled with carbohydrate (CHO) intake on players’ recovery from half-time break in a simulated soccer competition. Methods: In a single-blinded randomized crossover experiment, 14 male players received 3 treatments (Control: non-carbohydrate solution + travelling introduction audio; CHO: CHO–electrolyte solution + travelling introduction audio; and CHO_M: CHO–electrolyte solution + MBI) during simulated half-time breaks. Vertical jump, sprint performance, mindfulness level, rate of perceived exertion, muscle pain, mental fatigue, blood glucose, and lactate were measured immediately before, during, and after the exercise. Results: (1) MBI significantly increased participants’ mindfulness level (Control vs. CHO_M, p < 0.01; CHO vs. CHO_M, p < 0.01) and decreased mental fatigue for CHO_M condition (pre vs. post, p < 0.01); (2) participants in the CHO_M condition performed better in the repeated sprint tests than in the Control and CHO condition (Control vs. CHO_M, p = 0.02; CHO vs. CHO_M, p = 0.02). Conclusion: Findings of this study provide preliminary evidence of the positive effect of MBI coupled with CHO ingestion on athletes’ recovery from fatigue in the early stage of the second half of a game.