Sulfate Content of Carrageenan and Agar Determined by Infrared Spectroscopy

Rochas, C.; Lahaye, Marc; Yaphe, W.

doi:10.1515/botm.1986.29.4.335

Cited by 250 publications

(118 citation statements)

References 12 publications

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“…They present lower absorbance in the 805 cm (1 region (3,6-anhydro-D-galactose-2-sulphate), which indicates a presence of low quantities of i-carrageenan [5,6]. The ratio between 805 and 845 cm (1 absorption bands was calculated [5,7] and used as parameter to determine the degree of the kÁ/i hybridisation (Figs. 1, 3 and 4).…”

Section: Resultsmentioning

confidence: 99%

Carrageenophytes of occidental Portuguese coast: 1-spectroscopic analysis in eight carrageenophytes from Buarcos bay

Pereira

Mesquita

2003

Biomolecular Engineering

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Infrared and Raman spectroscopic analysis of the carrageenan (alkaline extraction) in eight species (representing seven genera and four families) of Gigartinales, in different reproductive phases from Buarcos bay (Figueira da Foz, Portugal), were studied. Female gametophytes and non-fertile thalli samples of Chondrus crispus , Mastocarpus stellatus , Chondracanthus teedei var. lusitanicus , Gigartina pistillata and Chondracanthus acicularis present a k-carrageenan profile or varying degrees of a k Á/i hybrid. The presence of k Á/i hybrid carrageenan in C. teedei var. lusitanicus was confirmed by 13 C NMR. The carrageenans extracted from Gymnogongrus crenulatus and Ahnfeltiopsis devoniensis are constituted mainly by i-carrageenan but seasonal variations in the nature of carrageenans are present. l-Family carrageenans were found in tetrasporophytes of C. crispus , M. stellatus , C. teedei var. lusitanicus , C. acicularis and G. pistillata . Calliblepharis jubata presents carrageenans of i-type in all reproductive stages. #

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Section: Resultsmentioning

confidence: 99%

Carrageenophytes of occidental Portuguese coast: 1-spectroscopic analysis in eight carrageenophytes from Buarcos bay

Pereira

Mesquita

2003

Biomolecular Engineering

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“…Several authors have used infrared spectroscopy (IR) to characterize and differentiate carrageenans κ, ι, and λ (HILLIOU et al, 2006;MENDOZA et al, 2006;PEREIRA et al, 2009;PRADO-FERNÁNDEZ et al, 2003;ROCHAS;LAHAYE;YAPHE, 1986). Figure 1a-c shows the FTIR spectra of native carrageenan and the carrageenan references for commercial and κ-carrageenan, respectively.…”

Section: Infrared Spectroscopymentioning

confidence: 99%

Optimization of the extraction of carrageenan from Kappaphycus alvarezii using response surface methodology

et al. 2012

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This study aims to optimize an alternative method of extraction of carrageenan without previous alkaline treatment and ethanol precipitation using Response Surface Methodology (RSM). In order to introduce an innovation in the isolation step, atomization drying was used reducing the time for obtaining dry carrageenan powder. The effects of extraction time and temperature on yield, gel strength, and viscosity were evaluated. Furthermore, the extracted material was submitted to structural analysis, by infrared spectroscopy and nuclear magnetic resonance spectroscopy (¹H-NMR), and chemical composition analysis. Results showed that the generated regression models adequately explained the data variation. Carrageenan yield and gel viscosity were influenced only by the extraction temperature. However, gel strength was influenced by both, extraction time and extraction temperature. Optimal extraction conditions were 74 ºC and 4 hours. In these conditions, the carrageenan extract properties determined by the polynomial model were 31.17%, 158.27 g.cm-2, and 29.5 cP for yield, gel strength, and viscosity, respectively, while under the experimental conditions they were 35.8 ± 4.68%, 112.50 ± 4.96 g.cm-2, and 16.01 ± 1.03 cP, respectively. The chemical composition, nuclear magnetic resonance spectroscopy, and infrared spectroscopy analyses showed that the crude carrageenan extracted is composed mainly of κ-carrageenan.

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“…The band at 928-933 cm-1 is connected with a coupling of the C-O stretching vibrations of 3,6-anhydro bridges [45,46]. This study shows that absorption peaks at 1257 and 845 cm-1 contribute to S=O of sulfate esters and C-O-S of axial secondary sulfate on C-4 of galactose, respectively.…”

Section: Ft-ir Spectramentioning

confidence: 55%

Chemical and Spectral Characterization of The Ozonation Products of κ-Carrageenan

et al. 2018

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Abstract. Kappa (κ-) carrageenan oligomers are known to have several biological activities. Recent progress in the development of modified κ-carrageenan has resulted low molecular of κ-carrageenan. Ozone is a powerful oxidant and considered for depolymerization of κ-carrageenan. However, few studies have investigated the changes in κ-carrageenan properties associated with ozone treatment. This study would investigate on the changes in chemical structure after ozonation process. The experiments were carried out in a glass reactor equipped with an ozone bubble diffuser. Ozone with concentration of 80 ± 2 was bubbled into the solution. The ozone treatment was conducted at different times, i.e., 0 (control), 5, 10, 15, and 20 minutes. The experiments were conducted at pH 7 and constant stirring speed (200 rpm). Ozone-treated κ-carrageenan was dried at 60 o C for 24 h in a forced air oven. The chemical and spectral analyses of κ-carrageenan after ozonation process were carried out using UV-Vis and FT-IR spectroscopy. These changes are seen in the UV spectra as a high intensity of absorbance peak at 290 nm. It is shows that ozonation of κ-carrageenan leads to some chemical changes such as the formation of carbonyl, carboxyl or double bonds.The FT-IR spectra reveals that the chemical structure of degraded κ-carrageenan, in term of sulfate content, is only slightly affected by the ozone treatment.

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Sulfate Content of Carrageenan and Agar Determined by Infrared Spectroscopy

Cited by 250 publications

References 12 publications

Carrageenophytes of occidental Portuguese coast: 1-spectroscopic analysis in eight carrageenophytes from Buarcos bay

Carrageenophytes of occidental Portuguese coast: 1-spectroscopic analysis in eight carrageenophytes from Buarcos bay

Optimization of the extraction of carrageenan from Kappaphycus alvarezii using response surface methodology

Chemical and Spectral Characterization of The Ozonation Products of κ-Carrageenan

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