Sulphur treatment alters the therapeutic potency of alliin obtained from garlic leaf extract

Alliin obtained from leaf extract of garlic grown underin situconditions possess higher therapeutic potency as analyzed in alloxan-induced diabetic rats

“…Leaves from plants grown under in situ condition showed noted enhancement ~50% in alliin production (Figure 2). Alliin accumulation depends upon plant variety and environmental conditions in which the plants are grown (Nasim et al, 2009a(Nasim et al, , 2010. Controlled growth and environmental conditions including light and temperature factors affect production of carbohydrates, thus influencing the production of alliin (Nasim et al, 2010).…”

“…Final dose administered corresponded to 0.5 g/kg body weight. The lower concentration was prepared according to the body weight of the rats by diluting the leaf extract with cold, sterile 0.9% saline (Nasim et al, 2009a). The present dose was decided on the basis of an earlier experiment that showed toxicity above the given value (data not shown).…”

“…The induction of diabetes in rats was done following methods described previously (Mansour et al, 2002;Nasim et al, 2009a).…”

“…The separation of serum was done as described previously (Nasim et al, 2009a). Serum collected was analyzed for enzyme levels immediately or within 24 h after storing at 4°C.…”

“…Garlic possesses high therapeutic and pharmacological properties including antibiotic, antitumor, antithrombotic, hypocholesterolemic, fibrinolytic, hypertensive effects (Eidi et al, 2005;El-Demerdash et al, 2005;Thomson et al, 2007;Nasim et al, 2009a). The hypoglycemic activity of plant contributing to treatment of diabetes has been attributed to S-allyl cysteine sulfoxide, the main active principle localized in cloves (Jones et al, 2007;Nasim et al, 2009b).…”