<sup>1</sup>H NMR studies of human blood plasma Assignment of resonances for lipoproteins

Bell, Jimmy D.; Sadler, Peter J.; Macleod, Andrew; Turner, Peter R.; Ville, A.E. La

doi:10.1016/0014-5793(87)81224-3

Cited by 94 publications

(41 citation statements)

References 12 publications

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“…Although no one has systematically studied the domain of preclinical breast cancer, our results agree with those of several other investigators (Bell et al, 1987;Nicholson & Nicholson, 1987;Small & Hamilton, 1987;Buchthal et al, 1988;Berger et al, 1989;Herring et al, 1989;Engan et al, 1990;Otvos et al, 1991).…”

Section: Data Collectionsupporting

confidence: 91%

Failure to detect early breast cancer using in vitro nuclear magnetic resonance spectroscopy of plasma

Holmberg

Jakobsson²,

Berglund³

et al. 1993

Br J Cancer

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Summary Water suppressed proton nuclear magnetic resonance ('H NMR) spectroscopy of human plasma has been described as successful in detection of malignancy. We designed a prospective study to test the hypothesis that in vitro NMR spectroscopy has a high sensitivity for detecting early breast cancer. One hundred and thirty-five women were referred for breast biopsy due to abnormal mammograms. One hundred of these were recruited through a population-based mammography screening project. Sixty-nine of 135 women were found to have breast cancer and their average line width of the methyl and methylene resonance in the plasma were compared to those women who had a benign or normal histopathology in the biopsy and to the line width for 100 healthy subjects from the same population. Thus the biopsy group was subdivided into one group with malignant and another with benign or normal histological findings. The sample size was predetermined to 120 by power calculations. Our aim was to detect differences in line width of the same magnitude as that described by Fossel, with 80% power and a 5% significance level. One hundred of the women were referred directly from a mammography screening center. The screening was a population-based public health care program for mammography alone.To obtain a reference group that was not subject to preoperative psychological stress, 100 healthy subjects from the same population were enrolled from an ongoing populationbased screening study for primary hyperparathyroidism among women 50 years of age and older. All these women gave their informed consent. Data collectionOn the day before surgery the women in the biopsy group were asked whether they had had a malignant tumour earlier in life, whether they smoked, whether they were on a special diet, when they had had their most recent meal and what it consisted of. Blood samples for determinations of the line width, serum triglycerides, serum cholesterol, serum FSH and estradiol were then drawn. Data about source of referral, histopathological diagnosis and tumour stage were obtained from the medical records.The healthy women were asked the same set of questions as those in the biopsy group. At the same time as the blood samples were drawn for the primary hyperparathyroidism screening, a blood sample for line width, serum triglycerides and serum cholesterol was drawn.A second blood sample was taken from 20 of the 69 patients with breast cancer 3-4 months after completion of therapy. These women had stage I or II cancer and were considered to be free of disease at the time.Laboratory methods EDTA-prepared tubes were used for blood sampling. The samples were centrifuged to obtain the plasma fraction within 60 min.

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Section: Data Collectionsupporting

confidence: 91%

Failure to detect early breast cancer using in vitro nuclear magnetic resonance spectroscopy of plasma

Holmberg

Jakobsson²,

Berglund³

et al. 1993

Br J Cancer

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“…In addition, the spectra of all synovial fluid samples examined contained fatty acid acyl chain terminal -CH, and bulk (-CHz-)n resonances (predominantly attributable to chylomicron-and very-low-density-lipoprotein (VLDL)-associated triacylglycerols [20]) which were of markedly weaker intensity than those present in corresponding spectra of matched serum samples (Fig. 1).…”

Section: Resultsmentioning

confidence: 98%

An investigation of the abnormal metabolic status of synovial fluid from patients with rheumatoid arthritis by high field proton nuclear magnetic resonance spectroscopy

et al. 1993

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The 'H Hahn spin-echo NMR profiles of rheumatoid synovial fluids have been investigated and compared with those of matched serum samples. In addition to markedly elevated lactate and diminished glucose concentrations, inflammatory synovial fluids contained (i) substantially lower levels ofNMR-detectable chylomicron-and very-low-density-lipoprotein-associated triacylglycerols which appear to have a shortened mean chain-length, and (ii) high concentrations of ketone bodies (predominantly 3-o-hydroxybutyrate), relative to those of corresponding paired serum samples. These observations confirm the abnormal metabolic status of the inflamed rheumatoid joint and provide evidence for an increased utilisation of lipids for fuel therein.

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“…However, this test for cancer is of little general use in view of the complexities of the lipoprotein resonances themselves [4,21,22].…”

Section: Discussionmentioning

confidence: 99%

NMR‐invisible lactate in blood plasma

et al. 1988

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Resonances for lactate are broadened in 500 MHz 1H NMR spectra of human blood plasma and only about one‐third is visible in Hahn spin‐echo spectra. Similar effects are observed for some other carboxylate anions. Lactate added to the high‐M r fraction of plasma can give rise to peaks which are too broad to observe in either single‐pulse or spin‐echo spectra. Addition of agents such as NH4Cl of SDS dramatically increases the intensities of lactate peaks. Some glycoproteins appear to broaden lactate resonances.

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¹H NMR studies of human blood plasma Assignment of resonances for lipoproteins

Cited by 94 publications

References 12 publications

Failure to detect early breast cancer using in vitro nuclear magnetic resonance spectroscopy of plasma

Failure to detect early breast cancer using in vitro nuclear magnetic resonance spectroscopy of plasma

An investigation of the abnormal metabolic status of synovial fluid from patients with rheumatoid arthritis by high field proton nuclear magnetic resonance spectroscopy

NMR‐invisible lactate in blood plasma

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1H NMR studies of human blood plasma Assignment of resonances for lipoproteins

Cited by 94 publications

References 12 publications

Failure to detect early breast cancer using in vitro nuclear magnetic resonance spectroscopy of plasma

Failure to detect early breast cancer using in vitro nuclear magnetic resonance spectroscopy of plasma

An investigation of the abnormal metabolic status of synovial fluid from patients with rheumatoid arthritis by high field proton nuclear magnetic resonance spectroscopy

NMR‐invisible lactate in blood plasma

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¹H NMR studies of human blood plasma Assignment of resonances for lipoproteins