Supercritical ammonia as mobile phase in capillary chromatography

Kuei, J. C.; Markides, Karin E.; Lee, M. L.

doi:10.1002/jhrc.1240100509

Cited by 48 publications

(12 citation statements)

References 20 publications

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“…This layer was used to immobilize p[HPMA] by a free radical cross-linking reaction. The effectiveness of this immobilization method has been demonstrated in GC and SFC column technologies [21,22], and in the preparation of composite biomaterials [23,24]. It is believed that the hydrogel polymer coating prepared by this method forms a uniform and highly cross-linked resin layer which is tightly bonded onto the capillary surface.…”

Section: Resultsmentioning

confidence: 99%

Hydrogel polymer coating for capillary electrophoretic separation of proteins

Huang

Lee

1992

J Microcolumn Separations

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Abstract. Three hydrogel polymers, poly(2-hydroxypropylmethacrylate), poly(2-hydroxyethylmethacrylate) , and hydroxypropylcellulose, were coated and immobilized on fused silica capillary surfaces using three different methods, all involving modification of the surfaces before polymerization and/or cross-linking. Since the hydrogel polymers swelled in aqueous solution and allowed permeation of low-molecular-weight compounds, an equilibrated state was attained between the hydrogel polymer layers and the buffer solution, which eliminated the interactions between proteins and the capillary surface, and allowed highly efficient and reproducible capillary electrophoretic separations of both basic and acidic proteins. The experimental results indicated that silanol groups on the capillary surface were covered and phosphate anions were trapped in the coating layers, which produced defined, but reduced, electroosmotic flows when compared to uncoated capillaries. High resolution separations of ribonuclease B variants and cytochrome c proteins from different sources were obtained.

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Section: Resultsmentioning

confidence: 99%

Hydrogel polymer coating for capillary electrophoretic separation of proteins

Huang

Lee

1992

J Microcolumn Separations

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“…Detection in the liquid state, by lowering the temperature, is in principle preferred to detection in the supercritical state, since supercritical fluids are more sensitive to refractiveindex changes as a function of density, compared to liquids. With narrow-bore columns, the detection is performed either on a fusedsilica tube with large diameter than the column, or directly on the column with the polyimide coating removed (Kuei et al, 1987;Fields et al, 1988). With 50 ~-tm i.d.…”

Section: 1 Uv and Fluorescence Detectionmentioning

confidence: 99%

Applications of Supercritical Fluids in Industrial Analysis

Dean¹

1993

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“…Supercritical ammonia, for example is highly polar, has a boiling point of -33.5 "C and reasonable critical parameters (T, 132.3 "C, P, 11 1.3 atm). It has also been shown to be a much stronger solvent than carbon dioxide for large polarizable PAC [26].…”

Section: Sfe-sfc Of Pac Standard Extracted Initially At Looatm (30 MImentioning

confidence: 99%

Supercritical fluid extraction / capillary supercritical fluid chromatography / Fourier transform infrared microspectrometry of polycyclic aromatic compounds in a coal tar pitch

Raynor

Davies

Bartle

et al. 1988

J. High Resol. Chromatogr.

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The combination of supercritical fluid extraction, high resolution capillary supercritical fluid chromatography, and Fourier transform infrared microspectrometry is described for the separation and identification of polycyclic aromatic hydrocarbons in a coal tar pitch. The variable solvating power of the supercritical fluid was utilized to selectively fractionate the sample. The fluid extract was decompressed through a frit restrictor into the sample cavity of a cooled microvalve injector, where the analytes were deposited and concentrated for subsequent chromatographic analysis. Several of the analytes separated in the chromatograph were collected on a potassium bromide disc at a solvent elimination interface for subsequent infrared analysis involving the use of an infrared microscope accessory. The spectra obtained show the power of this detection technique for distinguishing between isomers.

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Supercritical ammonia as mobile phase in capillary chromatography

Cited by 48 publications

References 20 publications

Hydrogel polymer coating for capillary electrophoretic separation of proteins

Hydrogel polymer coating for capillary electrophoretic separation of proteins

Applications of Supercritical Fluids in Industrial Analysis

Supercritical fluid extraction / capillary supercritical fluid chromatography / Fourier transform infrared microspectrometry of polycyclic aromatic compounds in a coal tar pitch

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