Superior Synaptogenic Effect of Electrospun PLGA-PEG Nanofibers Versus PLGA Nanofibers on Human Neural SH-SY5Y Cells in a Three-Dimensional Culture System

Kazemi, Leila; Rahbarghazi‬, Reza; Salehi, Roya; Abedelahi, Ali; Niari, Shabnam Asghari; Karimipour, Mohammad; Nasrabadi, Hamid Tayefi

doi:10.1007/s12031-020-01596-7

Cited by 12 publications

(13 citation statements)

References 40 publications

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“…Neuronal proliferation and differentiation are essential elements for providing a neural network in vitro 30 . We indicated that three conditions consisted of Reelin, PLGA‐PEG, and Reelin‐loaded PLGA‐PEG micelles encouraged proliferation and differential maturation of mouse NSCs after 7 and 14 days, respectively.…”

Section: Discussionmentioning

confidence: 97%

“…These changes coincided with an increased neurite number and length and branch points in Reelin‐loaded PLGA‐PEG micelles compared to the other groups. In our previous study, we noted that PLGA‐PEG nanofibers can accelerate neural differentiation and neurite growth in human neuroblastoma cells during 2 weeks in the in vitro condition 30 . It has been shown that Reelin phosphorylates S6 kinase 1 in mTORC1/PI3K‐dependent manner, which leads to dendritic growth and branching 58 .…”

Section: Discussionmentioning

confidence: 98%

“…In our previous study, we noted that PLGA‐PEG nanofibers can accelerate neural differentiation and neurite growth in human neuroblastoma cells during 2 weeks in the in vitro condition. 30 It has been shown that Reelin phosphorylates S6 kinase 1 in mTORC1/PI3K‐dependent manner, which leads to dendritic growth and branching. 58 These data showed that substrate composed of PLGA‐PEG is eligible to support neural growth and neurite formation and the addition of ECM glycoprotein, Reelin, acts as an anchor for cell attachment, migration, and development of cellular projections.…”

Section: Discussionmentioning

confidence: 99%

“…25 Therefore, the combining PLGA copolymer with non‐cytotoxic and hydrophilic PEG substrate offers the desired microenvironment for the generation of perineuronal net. 30 …”

Section: Introductionmentioning

confidence: 99%

“…One important avenue for PLGA functionalization is the attachment of polyethylene glycol (PEG) polymer chains known also as PEGylation 25 . Therefore, the combining PLGA copolymer with non‐cytotoxic and hydrophilic PEG substrate offers the desired microenvironment for the generation of perineuronal net 30 …”

Section: Introductionmentioning

confidence: 99%

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Transplantation of bioengineered Reelin‐loadedPLGA/PEGmicelles can accelerate neural tissue regeneration in photothrombotic stroke model of mouse

Shabani

Rahbarghazi‬

Karimipour

et al. 2021

Bioengineering & Transla Med

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Ischemic stroke is characterized by extensive neuronal loss, glial scar formation, neural tissue degeneration that leading to profound changes in the extracellular matrix, neuronal circuitry, and long‐lasting functional disabilities. Although transplanted neural stem cells (NSCs) can recover some of the functional deficit after stroke, retrieval is not complete and repair of lost tissue is negligible. Therefore, the current challenge is to use the combination of NSCs with suitably enriched biomaterials to retain these cells within the infarct cavity and accelerate the formation of a de novo tissue. This study aimed to test the regenerative potential of polylactic‐co‐glycolic acid‐polyethylene glycol (PLGA‐PEG) micelle biomaterial enriched with Reelin and embryonic NSCs on photothrombotic stroke model of mice to gain appropriate methods in tissue engineering. For this purpose, two sets of experiments, either in vitro or in vivo models, were performed. In vitro analyses exhibited PLGA‐PEG plus Reelin‐induced proliferation rate (Ki‐67 + NSCs) and neurite outgrowth (axonization and dendritization) compared to PLGA‐PEG + NSCs and Reelin + NSCs groups ( p < 0.05). Besides, neural differentiation (Map‐2 + cells) was high in NSCs cultured in the presence of Reelin‐loaded PLGA‐PEG micelles ( p < 0.05). Double immunofluorescence staining showed that Reelin‐loaded PLGA‐PEG micelles increased the number of migrating neural progenitor cells (DCX + cells) and mature neurons (NeuN + cells) around the lesion site compared to the groups received PLGA‐PEG and Reelin alone after 1 month ( p < 0.05). Immunohistochemistry results showed that the PLGA/PEG plus Reelin significantly decreased the astrocytic gliosis and increased local angiogenesis (vWF‐positive cells) relative to the other groups. These changes led to the reduction of cavity size in the Reelin‐loaded PLGA‐PEG+NSCs group. Neurobehavioral tests indicated Reelin‐loaded PLGA‐PEG+NSCs promoted neurological outcome and functional recovery ( p < 0.05). These results indicated that Reelin‐loaded PLGA‐PEG is capable of promoting NSCs dynamic growth, neuronal differentiation, and local angiogenesis following ischemic injury via providing a desirable microenvironment. These features can lead to neural tissue regeneration and functional recovery.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

“…25 Therefore, the combining PLGA copolymer with non‐cytotoxic and hydrophilic PEG substrate offers the desired microenvironment for the generation of perineuronal net. 30 …”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Transplantation of bioengineered Reelin‐loadedPLGA/PEGmicelles can accelerate neural tissue regeneration in photothrombotic stroke model of mouse

Shabani

Rahbarghazi‬

Karimipour

et al. 2021

Bioengineering & Transla Med

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Biomaterials patterning regulates neural stem cells fate and behavior: The interface of biology and material science

Niari

Rahbarghazi‬

Geranmayeh

et al. 2021

J Biomedical Materials Res

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The combination of nanotechnology and stem cell biology is one of the most promising advances in the field of regenerative medicine. This novel combination has widely been utilized in vitro settings in an attempt to develop efficient therapeutic strategies to overcome the limited capacity of the central nervous system (CNS) in replacing degenerating neural cells with functionally normal cells after the onset of acute and chronic neurological disorders. Importantly, biomaterials, not only, enhance the endogenous CNS neurogenesis and plasticity, but also, could provide a desirable supportive microenvironment to harness the full potential of the in vitro expanded neural stem cells (NSCs) for regenerative purposes. Here, first, we discuss how the physical and biochemical properties of biomaterials, such as their stiffness and elasticity, could influence the behavior of NSCs. Then, since the NSCs niche or microenvironment is of fundamental importance in controlling the dynamic destiny of NSCs such as their quiescent and proliferative states, topographical effects of surface diversity in biomaterials, that is, the micro-and nano-patterned surfaces will be discussed in detail. Finally, the influence of biomaterials as artificial microenvironments on the behavior of NSCs through the specific mechanotransduction signaling pathway mediated by focal adhesion formation will be reviewed.

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Polycaprolactone fumarate acts as an artificial neural network to promote the biological behavior of neural stem cells

et al. 2020

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Herein, we investigated the effect of electrospun polycaprolactone fumarate (PCLF) nanofibers on neural stem cell (NSC) behavior in the in vitro setting. Murine NSCs were isolated from adult mice subventricular zone and immunophenotyped by flow cytometry assay and immunofluorescence staining. Cells were cultured on the plastic surface, laminin-coated surface, and electrospun PCLF nanofibers. Cell morphology, attachment, and spreading were evaluated by scanning electron microscopy analysis. Cell viability and proliferation rates were evaluated by MTT assay. The proliferation of plated cells was investigated by monitoring Ki-67-positive cells using flow cytometry analysis. The protein levels of Map-2 and GFAP were detected by using immunofluorescence staining to show neural and astrocyte differentiation capacity. Scanning electron microscopy images revealed an extensive distribution, morphological adaptation, and cell-to-cell connectivity in NSCs upon culture on the PCLF surface. MTT analysis showed that the NSCs had more survival rates on the PCLF surface compared to the laminin and control groups over time (p < 0.05). In contrast to the laminin group, Ki-67 analysis showed a decrease of proliferating cells in the PCLF group. Immunofluorescence staining revealed the prominent increase of Map-2 and GFAP reduction in NSCs from the PCLF group compared to the laminin and control groups, showing the stimulatory effect of PCLF on targeted maturation of NSCs (p < 0.05). In brief, PCLF based construct promotes NSCs morphological adaptation and neuronal differentiation, suggesting PCLF as an appropriate and applicable substrate in neural tissue engineering.

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Superior Synaptogenic Effect of Electrospun PLGA-PEG Nanofibers Versus PLGA Nanofibers on Human Neural SH-SY5Y Cells in a Three-Dimensional Culture System

Cited by 12 publications

References 40 publications

Transplantation of bioengineered Reelin‐loadedPLGA/PEGmicelles can accelerate neural tissue regeneration in photothrombotic stroke model of mouse

Transplantation of bioengineered Reelin‐loadedPLGA/PEGmicelles can accelerate neural tissue regeneration in photothrombotic stroke model of mouse

Biomaterials patterning regulates neural stem cells fate and behavior: The interface of biology and material science

Polycaprolactone fumarate acts as an artificial neural network to promote the biological behavior of neural stem cells

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