Transplantation of bioengineered Reelin‐loaded<scp>PLGA</scp>/<scp>PEG</scp>micelles can accelerate neural tissue regeneration in photothrombotic stroke model of mouse

Shabani, Zahra; Rahbarghazi‬, Reza; Karimipour, Mohammad; Ghadiri, Tahereh; Salehi, Roya; Sadigh-Eteghad, Saeed; Farhoudi, Mehdi

doi:10.1002/btm2.10264

Cited by 22 publications

(7 citation statements)

References 71 publications

(135 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, Shabani et al [ 56 ] encapsulated NSCs with bioengineered Reelin-loaded polylactic coglycolic acidpolyethylene glycol (PLGA/PEG) micelles to retain the NSCs within the ischemic infarct cavity and accelerate the differentiation of these cells to generate de novo neuronal tissue after transplantation into a photothrombotic stroke model of mice[ 56 ]. Reelin, encoded by the RELN gene, is a large secreted ECM glycoprotein that regulates dendritic growth, dendritic spine development, synapse formation, and plasticity[ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Biomaterial application strategies to enhance stem cell-based therapy for ischemic stroke

Satar¹,

Othman²,

Tan³

2022

World J Stem Cells

View full text Add to dashboard Cite

BACKGROUND Ischemic stroke is a condition in which an occluded blood vessel interrupts blood flow to the brain and causes irreversible neuronal cell death. Transplantation of regenerative stem cells has been proposed as a novel therapy to restore damaged neural circuitry after ischemic stroke attack. However, limitations such as low cell survival rates after transplantation remain significant challenges to stem cell-based therapy for ischemic stroke in the clinical setting. In order to enhance the therapeutic efficacy of transplanted stem cells, several biomaterials have been developed to provide a supportable cellular microenvironment or functional modification on the stem cells to optimize their reparative roles in injured tissues or organs. AIM To discuss state-of-the-art functional biomaterials that could enhance the therapeutic potential of stem cell-based treatment for ischemic stroke and provide detailed insights into the mechanisms underlying these biomaterial approaches. METHODS The PubMed, Science Direct and Scopus literature databases were searched using the keywords of “biomaterial” and “ischemic stroke”. All topically-relevant articles were then screened to identify those with focused relevance to in vivo , in vitro and clinical studies related to “stem cells” OR “progenitor cells” OR “undifferentiated cells” published in English during the years of 2011 to 2022. The systematic search was conducted up to September 30, 2022. RESULTS A total of 19 articles matched all the inclusion criteria. The data contained within this collection of papers comprehensively represented 19 types of biomaterials applied on seven different types of stem/progenitor cells, namely mesenchymal stem cells, neural stem cells, induced pluripotent stem cells, neural progenitor cells, endothelial progenitor cells, neuroepithelial progenitor cells, and neuroblasts. The potential major benefits gained from the application of biomaterials in stem cell-based therapy were noted as induction of structural and functional modifications, increased stem cell retention rate in the hostile ischemic microenvironment, and promoting the secretion of important cytokines for reparative mechanisms. CONCLUSION Biomaterials have a relatively high potential for enhancing stem cell therapy. Nonetheless, there is a scarcity of evidence from human clinical studies for the efficacy of this bioengineered cell therapy, highlighting that it is still too early to draw a definitive conclusion on efficacy and safety for patient usage. Future in-depth clinical investigations are necessary to realize translation of this therapy into a more conscientious and judicious evidence-based therapy for clinical application.

show abstract

Section: Discussionmentioning

confidence: 99%

Biomaterial application strategies to enhance stem cell-based therapy for ischemic stroke

Satar¹,

Othman²,

Tan³

2022

World J Stem Cells

View full text Add to dashboard Cite

show abstract

“…As a non-cytotoxic amphiphilic polymer, PEG can endow PLGA with good hydrophilic properties and it provides suitable conditions for cells to grow [ 56 ]. PLGA-PEG composite hydrogels or electrospun fibers have been used for post-myocardial infarction tissue regeneration [ 7 ], neural engineering [ 40 , 57 , 58 ], and skeletal muscle regeneration [ 59 ]. Liu et al directly reprogrammed mouse embryonic fibroblasts (MEFs) into neural stem cells (iNSCs), and they demonstrated that the PLGA-PEG nanofiber scaffold was superior to the PLGA scaffold for iNSC adhesion and proliferation [ 58 ].…”

Section: Plga Scaffold Surface-modified Materialsmentioning

confidence: 99%

Surface Modification Progress for PLGA-Based Cell Scaffolds

Yan,

Hua,

Wang

et al. 2024

Polymers

View full text Add to dashboard Cite

Poly(lactic-glycolic acid) (PLGA) is a biocompatible bio-scaffold material, but its own hydrophobic and electrically neutral surface limits its application as a cell scaffold. Polymer materials, mimics ECM materials, and organic material have often been used as coating materials for PLGA cell scaffolds to improve the poor cell adhesion of PLGA and enhance tissue adaptation. These coating materials can be modified on the PLGA surface via simple physical or chemical methods, and coating multiple materials can simultaneously confer different functions to the PLGA scaffold; not only does this ensure stronger cell adhesion but it also modulates cell behavior and function. This approach to coating could facilitate the production of more PLGA-based cell scaffolds. This review focuses on the PLGA surface-modified materials, methods, and applications, and will provide guidance for PLGA surface modification.

show abstract

“…The Reelin-loaded PLGA-PEG + NSCs group showed a significantly higher NSC proliferation rate, neurite outgrowth, and neuronal differentiation compared to the PLGA-PEG + NSCs and Reelin + NSCs groups but decreased astrocytic gliosis and cavity size. Reelin-loaded PLGA-PEG + NSCs improved tissue regeneration and neurological outcomes [ 132 ].…”

Section: Applications Of Plga-based Nanostructures In Cell Transplant...mentioning

confidence: 99%

The Recent Applications of PLGA-Based Nanostructures for Ischemic Stroke

Yan,

Huang,

Feng

et al. 2023

Pharmaceutics

View full text Add to dashboard Cite

With the accelerated development of nanotechnology in recent years, nanomaterials have become increasingly prevalent in the medical field. The poly (lactic acid–glycolic acid) copolymer (PLGA) is one of the most commonly used biodegradable polymers. It is biocompatible and can be fabricated into various nanostructures, depending on requirements. Ischemic stroke is a common, disabling, and fatal illness that burdens society. There is a need for further improvement in the diagnosis and treatment of this disease. PLGA-based nanostructures can facilitate therapeutic compounds’ passage through the physicochemical barrier. They further provide both sustained and controlled release of therapeutic compounds when loaded with drugs for the treatment of ischemic stroke. The clinical significance and potential of PLGA-based nanostructures can also be seen in their applications in cell transplantation and imaging diagnostics of ischemic stroke. This paper summarizes the synthesis and properties of PLGA and reviews in detail the recent applications of PLGA-based nanostructures for drug delivery, disease therapy, cell transplantation, and the imaging diagnosis of ischemic stroke.

show abstract

Transplantation of bioengineered Reelin‐loadedPLGA/PEGmicelles can accelerate neural tissue regeneration in photothrombotic stroke model of mouse

Cited by 22 publications

References 71 publications

Biomaterial application strategies to enhance stem cell-based therapy for ischemic stroke

Biomaterial application strategies to enhance stem cell-based therapy for ischemic stroke

Surface Modification Progress for PLGA-Based Cell Scaffolds

The Recent Applications of PLGA-Based Nanostructures for Ischemic Stroke

Contact Info

Product

Resources

About