Superoxide dismutase activity is significantly lower in end-stage osteoarthritic cartilage than non-osteoarthritic cartilage

Abstract

Recent studies have shown that superoxide dismutase 1 (SOD1), SOD2, and SOD3 are significantly decreased in human osteoarthritic cartilage. SOD activity is a marker that can be used to comprehensively evaluate the enzymatic capacities of SOD1, SOD2, and SOD3; however, the trend of SOD activity in end-stage osteoarthritic tissues remains unknown. In the present study, we found that SOD activity in end-stage osteoarthritic synovium of the knee was significantly lower than that in control synovium without the influence of age. The SOD activity was significantly lower in the end-stage knee osteoarthritic cartilage than in the control, but a weak negative correlation was observed between aging and SOD activity. However, SOD activity in end-stage hip osteoarthritic cartilage was significantly lower than that in control cartilage without the influence of aging. The relationship between osteoarthritis and SOD activity was stronger than the relationship between aging and SOD activity. These results indicate that direct regulation of SOD activity in joint tissues may lead to suppression of osteoarthritis progression.

Association of Superoxide‐Induced Oxidative Stress With Rotator Cuff Tears in Human Patients

“…32 Therefore, it is important to determine each individual cause and evaluate the SOD activity comprehensively. 42 In this study, the total SOD activity was not significantly different between the Ruptured and Unruptured groups, although there was a weak correlation between the degeneration score and SOD activity. One of the factors for this result might be that the unruptured rotator cuff tissue is not a truly adequate control as it did display some degrees of degeneration, and SOD activity might have been affected by some degeneration.…”

“…SOD activity is regulated by both the amount of SOD protein and post‐translational modification (i.e., acetylation, nitration, phosphorylation, and glycation) causing decreased activity . Therefore, it is important to determine each individual cause and evaluate the SOD activity comprehensively . In this study, the total SOD activity was not significantly different between the Ruptured and Unruptured groups, although there was a weak correlation between the degeneration score and SOD activity.…”

“…There were some Ruptured group tissues in which SOD activity was low. Koike et al reported that SOD activity was decreased in end‐stage osteoarthritic cartilage. Similarly, in cases of advanced rotator cuff tears, it is suggested that the SOD might be inactivated and not compensable.…”

Effects of Superoxide Dismutase 1 on cartilage chondrocyte metabolism: a protective mechanism against ankle osteoarthritis?

“…Cells were seeded within media suspension (100,000/well of a 48-well plate) and subsequently treated (n=6) with 0.31U or 0.63U of bovine erythrocyte SOD1 (25U/mg and 50U/mg of cellular protein respectively). This represented the physiological concentrations of SOD1 reported for knee and ankle articular cartilage in the literature (6). SOD1 treatment occurred in the presence or absence of 5ng/ml IL-1 and 10ng/ml Oncostatin M treatment -used to mimic the pro-in ammatory catabolic response observed in OA; untreated cells served as controls.…”

Pathological Relationship between Intracellular Superoxide Metabolism and p53 Signaling in Mice

“…In mammals, copper/zinc-SOD (SOD1) exists in the cytoplasm, while manganese-SOD (SOD2) is distributed in the mitochondrial matrix to regulate intracellular redox balance in cells. Since SOD expression and activity are significantly decreased in aged osteoporotic, end-stage osteoarthritic, and Alzheimer’s disease individuals [ 4 , 5 , 6 ], redox imbalance caused by SOD decline is considered an important mechanism underlying the induction of age-related pathological changes.…”