Supplementation of lactobacilli improves growth, regulates microbiota composition and suppresses skeletal anomalies in juvenile pike-perch ( Sander lucioperca ) reared in recirculating aquaculture system (RAS): A pilot study

Ljubobratović, Uroš; Kosanovic, Dejana; Vukotić, Goran; Molnar, Zsuzsanna; Stanisavljević, Nemanja; Ristović, Tijana; Péter, Géza; Lukić, Jovanka; Jeney, Galina

doi:10.1016/j.rvsc.2017.07.018

Cited by 37 publications

(31 citation statements)

References 43 publications

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“…They are often efficacious, although most of this research has been conducted on other pathogens than Mycobacterium spp. [ 132 ]. Similarly, while no vaccine are currently commercially available, it is likely that autogenous vaccines could be applied to protect fish stocks although, once again, this will require more investigation to confirm.…”

Section: Discussionmentioning

confidence: 99%

Mycobacteriosis and Infections with Non-tuberculous Mycobacteria in Aquatic Organisms: A Review

2020

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The Mycobacteriaceae constitute a family of varied Gram-positive organisms that include a large number of pathogenic bacteria. Among these, non-tuberculous mycobacteria are endemic worldwide and have been associated with infections in a large number of organisms, including humans and other mammals and reptiles, as well as fish. In this review, we summarize the most recent findings regarding this group of pathogens in fish. There, four species are most commonly associated with disease outbreaks: Mycobacterium marinum, the most common of these fish mycobacterial pathogens, Mycobacterium fortuitum, Mycobacterium gordonae, and Mycobacterium chelonae. These bacteria have a broad host range: they are zoonotic, and infections have been reported in a large number of fish species. The main route of entry of the bacterium into the fish is through the gastrointestinal route, and the disease is associated with ulcerative dermatitis as well as organomegaly and the development of granulomatous lesions in the internal organs. Mycobacteriaceae are slow-growing and fastidious and isolation is difficult and time consuming and diagnostic is mostly performed using serological and molecular tools. Control of the disease is also difficult: there is currently no effective vaccine and infections react poorly to antibiotherapy. For this reason, more research is needed on the subject of these vexing pathogens.

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Section: Discussionmentioning

confidence: 99%

Mycobacteriosis and Infections with Non-tuberculous Mycobacteria in Aquatic Organisms: A Review

2020

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“…The issue of producing pikeperch (Sander lucioperca L.) juveniles suitable for intensive ongrowing has gained considerable research attention during the last two decades (Zakęś and Demska-Zakęś 1996;Kestemont et al 2007;Ljubobratović et al 2017a). Nevertheless, spawning induction of these fish after the grow-out phase has been the subject of a handful of studies thus far (Hermelink et al 2013(Hermelink et al , 2017Zakęś et al 2013).…”

Section: Introductionmentioning

confidence: 99%

The effect of hormonal preparation (gonadotropins vs. gonadoliberins) on pre-seasonally obtained eggs and larvae quality in pikeperch (Sander lucioperca L.)

et al. 2019

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Although several papers were dealing with intensively reared broodstocks, most of the published artificial reproduction protocols were applied in wild pikeperch breeders. Therefore, we aimed to evaluate the effects of ovulation induction agents, namely human chorionic gonadotropin (hCG) and salmon gonadotropin-releasing hormone analogue (sGnRHa) on the reproductive performance in pre-seasonal artificial propagation of cultured pikeperch. Breeders were harvested from an outdoor in-pond circulation system in mid-September and transferred to indoor facility in late January, conditioned and injected with either 50 μg kg −1 of sGnRHa or 500 IU kg −1 of hCG, with six females in each hormonal group. Stripped eggs from each female were sampled for chemical composition analysis, fertilised with the milt of two males, incubated and obtained larvae were further reared. No significant differences were noticed between the treatments in any of the analysed parameters of embryo survival nor larviculture performance. Rather high differences in intragroup variation were seen between the treatments, expressed in several-fold higher coefficients of variation in embryo survival in experimental and commercial conditions in the hCG group. Eggs obtained from females treated with hCG had a significantly higher lipid content (31.1% ± 2.0%) compared to sGnRHa-treated females (27.4% ± 1.8%). Fatty acid profiles of the eggs revealed differences in total n − 6 and linoleic acid 18:2n − 6 content while n − 3/n − 6 was significantly lower in the hCG group. Gonadoliberins reduced the variability in the spawning outcome. It appears that hormones can modify the eggs' fat content during the pre-season artificial reproduction.

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“…For feed treatment, bacteria were cultured until late logarithmic growth phase: 10 h for BGHN14 at 30°C and 12 h for BGT10 at 37°C in aerobic atmosphere. Cultivation of both strains was done in De Man, Rogosa and Sharpe medium (MRS) (Oxoid, Hamshire, UK) as explained previously [27].…”

Section: Methodsmentioning

confidence: 99%

“…The combination of two lactobacilli strains: Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10, which already showed the ability to hydrolyze proteins in fishmeal-based diet [27], was used. Different parameters related to lactobacilli application were varied, including total bacterial concentration, BGHN14 to BGT10 ratio, percentage of added saline and duration of incubation.…”

Section: Introductionmentioning

confidence: 99%

Solid state treatment with Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 improves nutrient bioavailability in granular fish feed

et al. 2019

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The aim of this research was to improve nutritive value of fishmeal-based feed by lactobacilli in order to achieve satisfactory nutrient availability needed to support fish development. Feed was solid-state treated at a laboratory scale with the combination of Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 in different experimental settings, which included the variation of strain ratio, total lactobacilli concentration, percentage of moisture and duration of incubation. Short peptides, soluble proteins, phospho-, neutral and unsaturated lipids were quantified. Differences among treated and control feeds were evaluated by Student t-test, while Gaussian process regression (GPR) modeling was employed to simulate the incubation process and define the optimal treatment combination in the context of overall feed nutritional profile. Treatment duration was shown to be the critical determinant of final outcome, either as single factor or via interaction with strain ratio. Optimal nutrient balance was achieved with 12 h incubation period, 260% moisture, 75:25 and 50:50 BGHN14:BGT10 ratios and 200 mg of lactobacilli per g of dry feed. This study should serve as the basis for large-scale tests which would simulate on-farm production of both fishmeal-based and unconventional, lower cost aquafeed with added value.

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Supplementation of lactobacilli improves growth, regulates microbiota composition and suppresses skeletal anomalies in juvenile pike-perch ( Sander lucioperca ) reared in recirculating aquaculture system (RAS): A pilot study

Cited by 37 publications

References 43 publications

Mycobacteriosis and Infections with Non-tuberculous Mycobacteria in Aquatic Organisms: A Review

Mycobacteriosis and Infections with Non-tuberculous Mycobacteria in Aquatic Organisms: A Review

The effect of hormonal preparation (gonadotropins vs. gonadoliberins) on pre-seasonally obtained eggs and larvae quality in pikeperch (Sander lucioperca L.)

Solid state treatment with Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 improves nutrient bioavailability in granular fish feed

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