Suppression of interleukin-2 gene expression by isoeugenol is mediated through down-regulation of NF-AT and NF-κB

Park, Kyung-Ran; Lee, Jung‐Hee; Choi, ChunYeon; Liu, Kwang-Hyeon; Seog, Dae‐Hyun; Kim, Yong-Ho; Kim, Dong-Eun; Yun, Chul‐Ho; Yea, Sung Su

doi:10.1016/j.intimp.2007.05.015

Cited by 24 publications

(15 citation statements)

References 31 publications

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“…Eugenol modulated cyclooxygenase (COX)-2 expression through the activation of NF-B in human osteoblasts (Lee, Yang, Ho, Lee, & Hung, 2007). Isoeugenol, another methoxyphenol component of clove oil, suppressed IL-2 production through the down-regulation of NF-B and NF-AT (Park et al, 2007).…”

Section: Resultsmentioning

confidence: 99%

In vitroandin vivoeffects of clove on pro-inflammatory cytokines production by macrophages

Rodrigues

Fernandes

Sousa³

et al. 2009

Natural Product Research

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Biological properties of clove have been reported, but little is known about its effect on the immune system. This work was aimed to investigate the effect in vivo of a water-soluble part of hydroalcoholic extract of clove on pro-inflammatory cytokines (IL-1beta and IL-6) production by macrophages of BALB/c mice. The action of the essential oil of clove on the production of these cytokines macrophages was also investigated in vitro. The chemical compositions of the extract and of the oil were also investigated. Treatment of mice with water extract of clove was found to inhibit macrophages to produce both IL-1beta and IL-6. The essential oil of clove also inhibited the production of these cytokines in vitro. Eugenol was found to be the major component of the clove extract and essential oil, and probably is the causative agent of cytokine inhibition. Taken together, these data suggest an anti-inflammatory action of this spice.

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Section: Resultsmentioning

confidence: 99%

In vitroandin vivoeffects of clove on pro-inflammatory cytokines production by macrophages

Rodrigues

Fernandes

Sousa³

et al. 2009

Natural Product Research

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“…It has been well established that activation of AhR by exogenous compounds, such as 3-methylcholanthrene and 2,3,7,8-tetrachlorodibenzo-p-dioxin, promotes the nuclear transport and transcriptional activation of CYP1A1 (Kawajiri & Fujii-Kuriyama, 2007;Pollenz, 2002). It is also been reported that the expression of hepatic CYP is reduced by lipopolysaccharide and cytokines, such as interleukin-1b and tumor necrosis factor-a, through the nuclear factor kappa B signaling pathway (Ke et al, 2001;Park et al, 2007). Indeed, further investigations need to be conducted to elucidate the detailed mechanisms underlying the suppression of CYP1A1 expression by eugenol.…”

Section: Discussionmentioning

confidence: 99%

Effect of dietary eugenol on xenobiotic metabolism and mediation of UDP-glucuronosyltransferase and cytochrome P450 1A1 expression in rat liver

Iwano¹,

Ujita²,

Nishikawa³

et al. 2013

International Journal of Food Sciences and Nutrition

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Xenobiotic-metabolizing enzymes (XMEs) play an important role in the elimination and detoxification of xenobiotics and drugs. A variety of natural dietary agents are known to protect against cancer by inducing XME. To elucidate the molecular mechanism of XME induction, we examined the effect of dietary eugenol (4-allyl-1-hydroxy-2-methoxybenzene) on xenobiotic metabolism. In this study, rats were administered dietary eugenol for 4 weeks to investigate the various effects of UDP-glucuronosyltransferase (UGT) and cytochrome P450 (CYP) expression. In rats administered dietary eugenol, expression levels of hepatic CYP1A 1 were reduced to 40% than of the controls, while expression of hepatic UGT1A6, UGT1A7 and UGT2B1 increased to 2-3 times than observed in the controls. Hepatic protein levels of UGT1A6 and 2B1 were also elevated in the eugenol-treated rats. These results suggest that the natural compound eugenol improves the xenobiotic-metabolizing systems that suppress and induce the expression of CYP1A1 and UGT, respectively.

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“…Following the induction of signaling cascades proximal to TCR ligation, nuclear factors translocate from the cytosol into the nucleus to elicit the expression of an array of cytokines. It is now appreciated that nuclear factor-kB (NF-kB) delivers a ''life signal'' by stimulating proinflammatory cytokines such as interleukin-2 (IL-2) and interferon-g in T-cells (14)(15)(16). IL-2 gene transcription, a hallmark feature of T-cell activation, is also regulated by activator protein-1 (AP-1) and nuclear factor of activated T-cell (NFAT) (17).…”

Section: Introductionmentioning

confidence: 99%

Dietary Curcumin and Limonin Suppress CD4+ T-Cell Proliferation and Interleukin-2 Production in Mice

Kim

Yang

Smith

et al. 2009

The Journal of Nutrition

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Phytochemicals may reduce chronic inflammation and cancer risk in part by modulating T-cell nuclear factor-kappaB (NF-kappaB) activation. Therefore, we examined the effects of curcumin (Cur) and limonin (Lim) feeding on NF-kappaB-dependent CD4(+) T-cell proliferation. DO11.10 transgenic mice (n = 5-7) were fed diets containing 1% Cur or 0.02% Lim combined with either (n-6) PUFA [5% corn oil (CO)] or (n-3) PUFA [4% fish oil+1% corn oil (FO)] for 2 wk, followed by splenic CD4(+) T-cell isolation and stimulation with ovalbumin peptide 323-339 (OVA) and antigen-presenting cells from mice fed a conventional nonpurified rodent diet. Both Cur and Lim diets suppressed (P < 0.05) NF-kappaB p65 nuclear translocation in activated CD4(+) T-cells. In contrast, activator protein-1 (c-Jun) and nuclear factor of activated T-cells c1 were not affected compared with the CO control diet (no Cur or Lim). CD4(+) T-cell proliferation in response to either mitogenic anti-CD3/28 monoclonal antibodies (mAb) or antigenic stimulation by OVA was also suppressed (P < 0.05) by Cur as assessed by carboxyfluorescein succinimidyl ester staining. In contrast, interleukin-2 production was not directly associated with NF-kappaB status. Interestingly, dietary combination with FO enhanced the suppressive effects (P < 0.05) of Cur or Lim with respect to CD4(+) T-cell proliferation in response to anti-CD3/28 mAb. These results suggest that combination chemotherapy (FO+Cur or Lim) may favorably modulate CD4(+) T-cell-mediated inflammation.

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Suppression of interleukin-2 gene expression by isoeugenol is mediated through down-regulation of NF-AT and NF-κB

Cited by 24 publications

References 31 publications

In vitroandin vivoeffects of clove on pro-inflammatory cytokines production by macrophages

In vitroandin vivoeffects of clove on pro-inflammatory cytokines production by macrophages

Effect of dietary eugenol on xenobiotic metabolism and mediation of UDP-glucuronosyltransferase and cytochrome P450 1A1 expression in rat liver

Dietary Curcumin and Limonin Suppress CD4+ T-Cell Proliferation and Interleukin-2 Production in Mice

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