Suppression of Interleukin-2 Receptor Expression on Mouse CD4⁺T Cells by Bovine κ-Caseinoglycopeptide

Abstract

8m-ine K-caseinoglycopeptide (residues lOfr169, CGP) completely inhibited the PH A-induced proliferation of mouse splenocytes when CGP was added simultaneously with PHA. The inhibitory effect, however, was reduced to about one-half when CGP was added after 24 h of cultinting the splenocytes with PHA or when anti-IL-Ira antibody was added simultaneously with PHA and CGP. On the other hand, CG P bound to mouse CD4 + T cells but not to CDS + T cells. CGP suppressed IL-2 receptor expression of the PHA-stimulated mouse CD4 + T cells.

Health effects and technological features of caseinomacropeptide

“…CMP also exerts immunomodulating activities, as it inhibits mitogens from inducing the proliferation of lymphocytes, and can even induce apoptosis of certain lymphocytes (Matin & Otani, 2000;Monnai & Otani, 1997;Otani, Horimoto, & Monnai, 1996;Otani, Monnai, Kawasaki, Kawakami, & Tanimoto, 1995). These immunomodulatory effects depend both on the polypeptide portion of CMP and on the presence of sialic acid (Li & Mine, 2004a;, but also on the immunological responsiveness of the mouse strain used for the cell proliferation assay (Otani, Horimoto, Matin, Ohnuki, & Kawahara, 2005).…”

Novel Mechanisms Underlying the Therapeutic Effect of Glycomacropeptide on Allergy: Change in Gut Microbiota, Upregulation of TGF-β, and Inhibition of Mast Cells

“…This peptide inhibits lipopolysaccharide and phytohemagglutinin-induced proliferation of mouse splenocytes and rabbit Peyer's patch cells [15], upregulates the expression of IL-1 receptor antagonist-like component in mouse lymphocytes [16] and suppresses the expression of IL-2 receptor on mouse CD4+ T cells [17]. It enhances the proliferation and the phagocytic activities of the human macrophage cell line U937 [18] and upregulates the secretion of tumor necrosis factor (TNF)-α, IL-1β, and IL-18 in human monocyte THP-1 cells [19].…”

Promoting Effect of Colostrum on the Phagocytic Activity of Bovine Polymorphonuclear Leukocytes in vitro

“…Therefore milk and colostrum have many bioactivity. Recently, it has been reported that kappa-casein and its digests in milk, which has been considered as just a protein nutrient, possess bioactive activity [7][8][9][10]. Furthermore, colostrum also contains living lymphocytes and mammary epithelial cells that express the mRNA of cytokines [11][12][13].…”