Suppression of LPS-induced epithelial-mesenchymal transition by aqueous extracts of Prunella vulgaris through inhibition of the NF-κB/Snail signaling pathway and regulation of EMT-related protein expression

Cho, In-Hye; Jang, Eun Hyang; Hong, Darong; Jung, Bom; Park, Min‐Ju; Kim, Jong-Ho

doi:10.3892/or.2015.4218

Cited by 11 publications

(6 citation statements)

References 30 publications

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“…We found that the overall variance was higher in treated cells versus untreated cells with an accompanying increase in the mean duration 23.7±4.73 and 21.7±3.42 hours, respectively (Figure 4a). Additionally, we also found that the addition of LPS appeared to enhance cell motility, as LPS treated cells showed an increase in overall cell displacement (Supplementary Figure 5), which supports the notion that LPS can induce epithelial-mesenchymal transition via TLR4 signaling [26][27][28] .…”

Section: Impact Of Lps On Cell Cycle Duration Variabilitysupporting

confidence: 79%

Impact of Variability in Cell Cycle Periodicity on Cell Population Dynamics

Nowak

Quarton

Bleris

2021

Preprint

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Cell cycle synchronization has been pivotal in the development of our understanding of cell population dynamics. Intriguingly, when cells are released from a synchronized state, they do not maintain synchronized cell division and rapidly become asynchronous. Here, using a combination of experiments and model simulations, we investigate this process of "cell cycle desynchronization" in cervical cancer cells (HeLa) that are arrested at the G1/S boundary. We tracked DNA content overtime at regular intervals to monitor cell cycle progression and developed a custom auto-similarity function to quantify the convergence to asynchronicity. In parallel, using experimental data, we developed a single-cell phenomenological model that returns DNA concentration across the cell cycle stages from a desynchronizing cell population. Our simulations revealed that desynchronization is primarily sensitive to cell cycle variability. We tested this prediction by introducing lipopolysaccharide to increase cellular noise, which resulted in greater cell cycle variability with an enhanced rate of desynchronization. Our results show that the desynchronization rate of cell populations can be used a proxy of the degree of variance in cell cycle periodicity.

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Section: Impact Of Lps On Cell Cycle Duration Variabilitysupporting

confidence: 79%

Impact of Variability in Cell Cycle Periodicity on Cell Population Dynamics

Nowak

Quarton

Bleris

2021

Preprint

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“…37 The exposure to LPS may induce tumor proliferation and facilitate invasion. 29,30,32 For example, Wang et al reported that hepatocellular cancer cell survival and proliferation were enhanced upon LPS stimulation. 38 Similarly, Yang et al demonstrated that LPS could induce cell proliferation in colorectal cancer.…”

Section: Discussionmentioning

confidence: 99%

“…LPS is known as an inducer of cell proliferation and invasion in various cancers. 29,30 Therefore, we examined the effect of MRPS23 down-regulation on LPS-induced proliferation and invasion of OS cells. The MTT and transwell assays were performed to measure cell proliferation and invasion, respectively.…”

Section: Down-regulation Of Mrps23 Induces Loss Of Mitochondrial Membmentioning

confidence: 99%

Down-regulation of MRPS23 inhibits LPS-induced proliferation and invasionviaregulation of the NF-κB signaling pathway in osteosarcoma cells

Liu

Wang

et al. 2019

RSC Adv.

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Mitochondrial ribosomal protein S23 (MRPS23), encoded by a nuclear gene, is a participant in the translation of mitochondrial proteins. Recently, MRPS23 has been reported to be overexpressed in many types of cancers and have a close association with cancer progression. However, the specific roles of MRPS23 in osteosarcoma (OS) remain unknown. In this study, we investigated the expression pattern and biological functions of MRPS23 in OS cells. Our results demonstrated that MRPS23 was up-regulated in OS tissues and cell lines. Down-regulation of MRPS23 significantly inhibited OS cell proliferation and invasion induced by lipopolysaccharide (LPS) in vitro. Furthermore, the in vivo experiments showed that MRPS23 down-regulation markedly suppressed OS cell growth and metastasis induced by LPS. Mechanistically, down-regulation of MRPS23 inhibited the activity of NF-kB signaling pathway in OS cells. In conclusion, these findings indicated that MRPS23 may be a potential therapeutic target for OS treatment. Results Expression of MRPS23 is elevated in OS tissues and cell linesThe expression of MRPS23 in OS tissues was examined by immunohistochemistry, RT-PCR and western blot analysis. The

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“…Strikingly, PV annulled such inflammatory responses induced by dsDNA or dsRNA in thyrocytes, and provided an exceptional protective effect in the immune-activated thyrocytes. Such antiinflammatory action of PV in thyrocytes, in addition to its previously documented immunomodulatory effects in macrophages (26,27,29), may help to explain the observed therapeutic efficacy of PV for Hashimoto's thyroiditis. In turn, our new findings highlight that innate immune responses in thyrocytes triggered by dsDNA or dsRNA is likely a critical factor for precipitating thyroid autoimmunity, and herbal/ compound agents (i.e.…”

Section: Discussionmentioning

confidence: 81%

Innate Immune-Modulatory Activity of Prunella vulgaris in Thyrocytes Functions as a Potential Mechanism for Treating Hashimoto’s Thyroiditis

et al. 2020

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Prunella vulgaris (PV), a perennial herb, has been used to treat thyroid diseases in China for over 2,000 years. In particular, its therapeutic effect has been described for Hashimoto’s thyroiditis, including reducing titers autoantibodies against thyroid peroxidase and thyroglobulin of and T helper 17 (Th17) cells. However, the underlying mechanism for how PV exerts such effects has not been investigated. We examined the effects of PV on innate immune activation, which is thought to be one of the triggers for the development of autoimmune diseases, including Hashimoto’s thyroiditis. In cultured thyrocytes, PV reduced mRNA levels of inflammatory cytokines that were originally induced as a result of innate immune activation initiated by transfection of double-stranded DNA (dsDNA) or dsRNA. PV suppressed activation of nuclear factor κB (NF-κB) and interferon regulatory factor 3 (IRF3), and suppressed corresponding promoter activation, which were initially activated by dsDNA or dsRNA. PV also suppressed the mRNA levels of molecules responsible for antigen processing and presentation, and PV protected thyrocytes from apoptosis induced by dsDNA and dsRNA. Additionally, PV suppressed the expression of genes involved in iodide uptake and oxidation. Taken together, these results suggest that PV exerts its protective effect on thyrocytes by suppressing both innate and adaptive immune responses and cell death. PV may also protect cells from iodide-associated oxidative injury. This report is among the first to identify the mechanisms to explain PV’s beneficial effects in Hashimoto’s thyroiditis.

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Suppression of LPS-induced epithelial-mesenchymal transition by aqueous extracts of Prunella vulgaris through inhibition of the NF-κB/Snail signaling pathway and regulation of EMT-related protein expression

Cited by 11 publications

References 30 publications

Impact of Variability in Cell Cycle Periodicity on Cell Population Dynamics

Impact of Variability in Cell Cycle Periodicity on Cell Population Dynamics

Down-regulation of MRPS23 inhibits LPS-induced proliferation and invasionviaregulation of the NF-κB signaling pathway in osteosarcoma cells

Innate Immune-Modulatory Activity of Prunella vulgaris in Thyrocytes Functions as a Potential Mechanism for Treating Hashimoto’s Thyroiditis

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