Suppression of Peritoneal Fibrosis by Sonoporation of Hepatocyte Growth Factor Gene-Encoding Plasmid DNA in Mice

Nishimura, Kayoko; Ogawa, Kazuhiro; Kawaguchi, Maho; Fumoto, Shintaro; Mukai, Hidefumi; Kawakami, Shigeru

doi:10.3390/pharmaceutics13010115

Cited by 13 publications

(6 citation statements)

References 35 publications

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“…Moreover, the application of ultrasound damages cells, altering enzymatic and mitochondrial activity and resulting in apoptosis [68]. In contrast to reports with naked DNA and electroporation, in some cases, expression of transgenes introduced via sonoporation was only detectable for a day following administration before returning to pretreatment levels [69]. When compared directly to naked pDNA delivery, there was no significant difference in the expression of introduced transgenes, although sonoporation resulted in successful gene transfer in more animals per treatment group [70].…”

Section: Sonoporationmentioning

confidence: 93%

Exosomes as an Emerging Plasmid Delivery Vehicle for Gene Therapy

et al. 2023

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Despite its introduction more than three decades ago, gene therapy has fallen short of its expected potential for the treatment of a broad spectrum of diseases and continues to lack widespread clinical use. The fundamental limitation in clinical translatability of this therapeutic modality has always been an effective delivery system that circumvents degradation of the therapeutic nucleic acids, ensuring they reach the intended disease target. Plasmid DNA (pDNA) for the purpose of introducing exogenous genes presents an additional challenge due to its size and potential immunogenicity. Current pDNA methods include naked pDNA accompanied by electroporation or ultrasound, liposomes, other nanoparticles, and cell-penetrating peptides, to name a few. While the topic of numerous reviews, each of these methods has its own unique set of limitations, side effects, and efficacy concerns. In this review, we highlight emerging uses of exosomes for the delivery of pDNA for gene therapy. We specifically focus on bovine milk and colostrum-derived exosomes as a nano-delivery “platform”. Milk/colostrum represents an abundant, scalable, and cost-effective natural source of exosomes that can be loaded with nucleic acids for targeted delivery to a variety of tissue types in the body. These nanoparticles can be functionalized and loaded with pDNA for the exogenous expression of genes to target a wide variety of disease phenotypes, overcoming many of the limitations of current gene therapy delivery techniques.

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Section: Sonoporationmentioning

confidence: 93%

Exosomes as an Emerging Plasmid Delivery Vehicle for Gene Therapy

et al. 2023

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“…A sonoporation-based human Hepatocyte growth factor (hHGF) gene transfection was attempted in the peritoneal fibrosis model of mice. This showed a preventive and curative effect as gene expression was observed in the submesothelial layer which is suitable for treating early stage peritoneal fibrosis …”

Section: Interaction Of Ultrasound With Biological Systems: Physical ...mentioning

confidence: 99%

“…95 and curative effect as gene expression was observed in the submesothelial layer which is suitable for treating early stage peritoneal fibrosis. 96 In vitro studies on colon and rectum cells do not represent the complexity of heterogeneous intestinal epithelium. Hence, Tarcanu and colleagues grew a confluent cell layer from rectal OGs on inserts to compare the effects between US+MBs (MBs) and US+nanodroplets (NDs) for insulin permeation into intestinal barrier insulin.…”

Section: Interaction Of Ultrasound Withmentioning

confidence: 99%

Ultrasound-Enabled Therapeutic Delivery and Regenerative Medicine: Physical and Biological Perspectives

Bansal

Jha

Bhatia

et al. 2021

ACS Biomater. Sci. Eng.

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The role of ultrasound in medicine and biological sciences is expanding rapidly beyond its use in conventional diagnostic imaging. Numerous studies have reported the effects of ultrasound on cellular and tissue physiology. Advances in instrumentation and electronics have enabled successful in vivo applications of therapeutic ultrasound. Despite path breaking advances in understanding the biophysical and biological mechanisms at both microscopic and macroscopic scales, there remain substantial gaps. With the progression of research in this area, it is important to take stock of the current understanding of the field and to highlight important areas for future work. We present herein key developments in the biological applications of ultrasound especially in the context of nanoparticle delivery, drug delivery, and regenerative medicine. We conclude with a brief perspective on the current promise, limitations, and future directions for interfacing ultrasound technology with biological systems, which could provide guidance for future investigations in this interdisciplinary area.

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“…Physical methods are the most common administration strategies, including electroporation, gene gun and sonoporation, which lead to the difficulties in clinical promotion. [101][102][103] DNA vaccines have demonstrated limited immunogenicity in clinical trials, despite their effective delivery. Efforts have been made to enhance the efficacy of DNA vaccines through various strategies.…”

Section: Vaccine Constituent and Platformmentioning

confidence: 99%

Therapeutic cancer vaccines: advancements, challenges and prospects

Fan,

Zhang,

Yang

et al. 2023

Sig Transduct Target Ther

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With the development and regulatory approval of immune checkpoint inhibitors and adoptive cell therapies, cancer immunotherapy has undergone a profound transformation over the past decades. Recently, therapeutic cancer vaccines have shown promise by eliciting de novo T cell responses targeting tumor antigens, including tumor-associated antigens and tumor-specific antigens. The objective was to amplify and diversify the intrinsic repertoire of tumor-specific T cells. However, the complete realization of these capabilities remains an ongoing pursuit. Therefore, we provide an overview of the current landscape of cancer vaccines in this review. The range of antigen selection, antigen delivery systems development the strategic nuances underlying effective antigen presentation have pioneered cancer vaccine design. Furthermore, this review addresses the current status of clinical trials and discusses their strategies, focusing on tumor-specific immunogenicity and anti-tumor efficacy assessment. However, current clinical attempts toward developing cancer vaccines have not yielded breakthrough clinical outcomes due to significant challenges, including tumor immune microenvironment suppression, optimal candidate identification, immune response evaluation, and vaccine manufacturing acceleration. Therefore, the field is poised to overcome hurdles and improve patient outcomes in the future by acknowledging these clinical complexities and persistently striving to surmount inherent constraints.

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Suppression of Peritoneal Fibrosis by Sonoporation of Hepatocyte Growth Factor Gene-Encoding Plasmid DNA in Mice

Cited by 13 publications

References 35 publications

Exosomes as an Emerging Plasmid Delivery Vehicle for Gene Therapy

Exosomes as an Emerging Plasmid Delivery Vehicle for Gene Therapy

Ultrasound-Enabled Therapeutic Delivery and Regenerative Medicine: Physical and Biological Perspectives

Therapeutic cancer vaccines: advancements, challenges and prospects

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