Suppressive Effect of Kampo Formula “Juzen-taiho-to” on Carbon Tetrachloride-Induced Hepatotoxicity in Mice

Yoshioka, Hiroki; Fukaya, Shiori; Miura, Nobuhiko; Onosaka, Satomi; Nonogaki, Tsunemasa; Nagatsu, Akito

doi:10.1248/bpb.b16-00421

Cited by 17 publications

(15 citation statements)

References 26 publications

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“…Moreover, i.p. administration of JTX induces an increase in hepatic MT (Yoshioka et al, 2016a). Our results showed that administration of JTX did not increase in the hepatic MT levels, which suggests that MT does not involve a protective role in the present investigation.…”

Section: Discussionsupporting

confidence: 42%

“…We measured the plasma levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) using the Transaminase CII Test Wako (Wako Pure Chemical, Osaka, Japan) according to the manufacturer's instructions and as previously described (Yoshioka et al, 2016a).…”

Section: Biochemical Analysis Of Plasmamentioning

confidence: 99%

“…The results of our previous study showed that pretreatment with JTX prevents acute liver disease (Yoshioka et al, 2016a) induced by carbon tetrachloride, which is an established agent that induces liver. Thus, our results suggest that JTX, which contains various active ingredients, may attenuate several kinds of hepatic diseases.…”

Section: Introductionmentioning

confidence: 99%

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The Kampo formula “Juzen-taiho-to” exerts protective effects on ethanol-induced liver injury in mice

Fukaya

Nagatsu

Yoshioka

2018

Fundam. Toxicol. Sci.

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-The aim of this study was to investigate whether the Japanese herbal medicine "Juzen-taiho-to (JTX)" has a protective effect on ethanol (EtOH)-induced liver injury. Seven-week-old male ICR mice were orally administered JTX or saline once a day for three days. Twenty-four hours after the last administration, the mice were intraperitoneally injected with EtOH (2 g/kg). The mice in each group were killed 24 hr after EtOH administration and were bled to obtain plasma. The mice injected with EtOH had high plasma levels of alanine aminotransferase and aspartate aminotransferase and lipid peroxidation. Histopathological examination of the liver of mice treated with EtOH revealed an abnormal outline around the central vein, glycogen depletion, and expression of prostaglandin-endoperoxide synthase 2. Pretreatment with JTX prevented the EtOH-induced increase in the levels of alanine aminotransferase and aspartate aminotransferase, lipid peroxidation, and histopathological changes. Our results suggest that JTX exerts protective effects against EtOH-induced liver disease by modulating oxidative stress and inflammatory response.

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Section: Discussionsupporting

confidence: 42%

Section: Biochemical Analysis Of Plasmamentioning

confidence: 99%

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The Kampo formula “Juzen-taiho-to” exerts protective effects on ethanol-induced liver injury in mice

Fukaya

Nagatsu

Yoshioka

2018

Fundam. Toxicol. Sci.

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“…Total malondialdehyde (MDA) levels were estimated by a colorimetric microplate assay (Oxford Biochemical Research, Oxford, MI, USA) according to the manufacturer's protocol and as previously described (Yoshioka et al, 2016a(Yoshioka et al, , 2016b.…”

Section: Measurement Of Malondialdehyde Levels and Total Antioxidant mentioning

confidence: 99%

Multidirectional analyses of hepatic chronotoxicity induced by cadmium in mice

et al. 2017

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-The expression levels or activities of biological defense factors can fluctuate daily following biological rhythms. We have focused on the relationship between injection timing and the degree of toxicity of cadmium (Cd) to promote the concept of "chronotoxicology," which introduces chronobiology to the field of toxicology. Our previous studies have clearly indicated that Cd may be subject to chronotoxicity. In this report, to confirm the character of the Cd-induced chronotoxicity, we performed multidirectional examinations. Male C57BL/6J mice that received a single intraperitoneal injection of CdCl 2 at ZT6 showed drastic hepatic injury estimated by histopathological analyses, i.e., nuclear condensations, fatty degenerations, and hemorrhages, but showed no injury when injected at ZT18. This difference was supported by several biochemical analyses that were indicators of hepatic injury (levels of alanine aminotransferase, aspartate aminotransferase, and malondialdehyde). The chronotoxicity of Cd was also observed in multiple strains (ICR and Balb/c), in a different injection route (subcutaneous), and in multiple injections (5 injections). Based on these results, we propose that chronotoxicology may provide important information not only for toxicology but also for occupational health, i.e., the importance of injection timing for toxicity evaluation tests, the reproducibility of animal experiments, and the improvement in the quality of risk assessments for night shift workers who may be exposed to toxic substances at various times of the day.

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“…The levels of these enzymes provide a sensitive diagnostic for liver damage (Kim et al, ). Similarly, increased serum AKP is associated with the liver cell membrane injury (Yoshioka et al, ). Increased serum γ‐GT can result in the production of free radicals (Chen et al, ).…”

Section: Discussionmentioning

confidence: 99%

Herbal Formula, Baogan Yihao (BGYH), Prevented Dimethylnitrosamine(DMN)‐Induced Liver Injury in Rats

Liu

Shi

et al. 2017

Drug Development Research

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Preclinical Research Baogan Yihao (BGYH) is a traditional Chinese herbal medicine for the treatment of chronic liver diseases. In this study, the effects of BGYH on dimethylnitrosamine (DMN)-induced liver fibrosis were investigated using a rat model. BGYH alleviate liver damage, as indicated by decreased levels of AST, ALT, γ-GT, and AKP. BGYH also prevented collagen deposition and reduced pathological tissue injury in liver tissue. In fibrosis, high levels of α-SMA and TGF-β in liver tissue were markedly attenuated by BGYH. The inhibitory effect of BGYH on HSC-T6 proliferation demonstrated that BGYH exhibited significant hepatoprotective and antifibrogenic effects on DMN-induced liver injury. These findings suggest that BGYH may have therapeutic potential in the prevention and therapy of liver fibrosis. Drug Dev Res 78 : 155-163, 2017. © 2017 Wiley Periodicals, Inc.

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Suppressive Effect of Kampo Formula “Juzen-taiho-to” on Carbon Tetrachloride-Induced Hepatotoxicity in Mice

Abstract: The aim of the present study was to investigate whether pretreatment with the Japanese herbal medicine, "Juzen-taiho-to" (JTX), had an ameliorative effect on carbon tetrachloride (

Cited by 17 publications

References 26 publications

The Kampo formula “Juzen-taiho-to” exerts protective effects on ethanol-induced liver injury in mice

The Kampo formula “Juzen-taiho-to” exerts protective effects on ethanol-induced liver injury in mice

Multidirectional analyses of hepatic chronotoxicity induced by cadmium in mice

Herbal Formula, Baogan Yihao (BGYH), Prevented Dimethylnitrosamine(DMN)‐Induced Liver Injury in Rats

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