Suppressor cells in rabbit peripheral blood

Abstract

Rabbit peripheral blood leukocytes (PBL) added to cultures of autologous spleen cells, primed in vivo to sheep red cells, are able to suppress with high efficacy the secondary in vitro response of the spleen cells to that antigen. Removal of nylon wool adherent cells from PBL abolishes the suppressive effect. When the PBL are fractionated by velocity sedimentation the suppressor cells can be separated from the responding cells. The circulating lymphocytes, freed from the inhibiting effect, either by nylon wool absorption or by velocity sedimentation fractionation, are able to give a strong secondary in vitro anti-SRC response, in which the long latency period, usually observed when PBL are stimulated with antigen in culture, is abolished or at least reduced. The suppressor effect present in the PBL is not due to granulocytes, platelets or erythrocytes.

Induction of an antibody response in cultures of human peripheral blood lymphocytes.

“…We report evidence that the number of such cells must be relatively high, in fact no fluctuation of the response was observed among different cultures when culturing 10 ~ cells/well, thus indicating that each well must have received at least one precursor cell. Triggering can occur only after removal of adhering inhibitor cells, which are likely to be analogous to the adhering inhibitor cells previously described in rabbit PBL (11).…”

“…The number of direct (IgM) and indirect (IgG) PFC in the cultures was assayed using a modification of the method originally described by Jerne et al (16) and adapted for microscope slides by Mishell and Dutton (14) as previously described in detail (11). Briefly, 20 ~l of a 10 mg/ml solution of DEAE-dextran (Pharmacia Fine Chemicals, Inc.), 20 ~l of 20% SRBC or HRBC, and 10-50/xl of cell suspension were added to tubes containing 0.5 ml of 0.7% agar at 46°C.…”

Histamine‐binding suppressor T cells in rabbit peripheral blood

“…However, this response was found to be under the control of very active suppressor cells [3]. The presence of suppressor cells in the blood or lymphoid organs has been demonstrated by a number of investigators for different mammalian species including humans ( [3][4][5]*).…”

“…However, this response was found to be under the control of very active suppressor cells [3]. The presence of suppressor cells in the blood or lymphoid organs has been demonstrated by a number of investigators for different mammalian species including humans ( [3][4][5]*). The existence of certain cell types regulating the network of lymphoid cell interactions was demonstrated using different cell fractionation methods which selectively removed a certain regulating cell type.…”

“…The existence of certain cell types regulating the network of lymphoid cell interactions was demonstrated using different cell fractionation methods which selectively removed a certain regulating cell type. Indeed, suppressive cells have been isolated by rosetting with IgG-coated erythrocytes [4], by adherence to glass wool [6] or to antigencoated dishes [7], by velocity sedimentation [3] and by selective complement (C)-mediated killing with anti-Ly antisera [8,91. In the murine system, one efficient way to separate suppressor cells has been the fractionation of lymphoid cells on columns of insolubilized histamine [lo, 111.…”