Suppressor of RNA silencing encoded by Rice gall dwarf virus genome segment 11

FuXiu, Liu; Zhao, Qin; Ruan, Xiaolei; He, Yunwei; Li, Huaping

doi:10.1007/s11434-008-0095-x

Cited by 11 publications

(10 citation statements)

References 40 publications

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“…To date, the function of the protein encoded by S12 and Pns12 remains unknown. A previous report showed that RGDV S11 encodes a silencing suppressor [21]. In this study, we present evidence that Pns12 of RGDV also has silencing suppressor activities.…”

Section: Rice Gall Dwarf Virus (Rgdv) Is a Member Of The Genussupporting

confidence: 66%

“…In addition, Cui et al [47] demonstrated that a Geminivirus, Tobacco curly shoot virus Y35 (TbCSV-Y35), could process three VSRs, namely AC2, AC4, and βC1. Liu et al [21] have reported that RGDV Pns11 was a silencing suppressor.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, some VSRs have been shown to interfere with the movement of silencing signals. A number of VSRs have been identified from plant and animal viruses [15][16][17][18][19][20][21][22][23][24][25]. Although most plant viruses only have one VSR, some plant viruses encode more than one silencing suppressor, which target distinct steps of RNA silencing [26,27].…”

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confidence: 99%

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Identification of Pns12 as the second silencing suppressor of Rice gall dwarf virus

Wang

et al. 2011

Sci. China Life Sci.

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RNA silencing is a conserved mechanism found ubiquitously in eukaryotic organisms. It has been used to regulate gene expression and development. In addition, RNA silencing serves as an important mechanism in plants' defense against invasive nucleic acids, such as viruses, transposons, and transgenes. As a counter-defense, most plants, and some animal viruses, encode RNA silencing suppressors to interfere at one or several points of the silencing pathway. In this study, we showed that Pns12 of RGDV (Rice gall dwarf virus) exhibits silencing suppressor activity on the reporter green fluorescent protein in transgenic Nicotiana benthamiana line 16c. Pns12 of RGDV suppressed local silencing induced by sense RNA but had no effect on that induced by dsRNA. Expression of Pns12 also enhanced Potato virus X pathogenicity in N. benthamiana. Collectively, these results suggested that RGDV Pns12 functions as a virus suppressor of RNA silencing, which might target an upstream step of dsRNA formation in the RNA silencing pathway. Furthermore, we showed that Pns12 is localized mainly in the nucleus of N. benthamiana leaf cells.

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Section: Rice Gall Dwarf Virus (Rgdv) Is a Member Of The Genussupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Identification of Pns12 as the second silencing suppressor of Rice gall dwarf virus

Wang

et al. 2011

Sci. China Life Sci.

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“…Most viruses can achieve self-assembly effectively in eukaryotic expression systems. For example, Orthoreovirus [10] , Rotavirus [22] and Bluetongue virus [9] in the Reoviridae family can assemble into single-, double-or triple- experiments, such as S1 fragment encoding RNAdependent RNA polymerases (RdRp) [13] , S5-encoded minor core protein having the guanylyltransferase activity [8] , and S11 encoding an RNA silencing suppressor [11] , etc. In the genus Phytoreovirus, the structure and assembly mechanism of RDV particles have been most clearly described.…”

Section: Discussionmentioning

confidence: 99%

Expression of rice gall dwarf virus outer coat protein gene (S8) in insect cells

et al. 2010

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To obtain the P8 protein of Rice gall dwarf virus (RGDV) with biological activity, its outer coat protein gene S8 was expressed in Spodoptera frugiperda (Sf9) insect cells using the baculovirus expression system. The S8 gene was subcloned into the pFastBac™1 vector, to produce the recombinant baculovirus transfer vector pFB-S8. After transformation, pFB-S8 was introduced into the competent cells (E. coli DH10Bac) containing a shuttle vector, Bacmid, generating the recombinant bacmid rbpFB-S8. After being infected by recombinant baculovirus rvpFB-S8 at different multiplicities of infection, Sf9 cells were collected at different times and analyzed by SDS-PAGE, Western blotting and immunofluorescence microscopy. The expression level of the P8 protein was highest between 48-72 h after transfection of Sf9 cells. Immunofluorescence microscopy showed that P8 protein of RGDV formed punctate structures in the cytoplasm of Sf9 cells.

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“…In the case of rice-infecting reoviruses, Pns10 encoded by RDV segment 10 and Pns11 encoded by RGDV segment 11, have been shown to have RNA silencing suppressor activity (Cao et al, 2005; Zhang et al, 2005; Liu et al, 2008). …”

Section: Conferring Resistance Against Rice-infecting Reovirusesmentioning

confidence: 99%

Transgenic strategies to confer resistance against viruses in rice plants

et al. 2014

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Rice (Oryza sativa L.) is cultivated in more than 100 countries and supports nearly half of the world’s population. Developing efficient methods to control rice viruses is thus an urgent necessity because viruses cause serious losses in rice yield. Most rice viruses are transmitted by insect vectors, notably planthoppers and leafhoppers. Viruliferous insect vectors can disperse their viruses over relatively long distances, and eradication of the viruses is very difficult once they become widespread. Exploitation of natural genetic sources of resistance is one of the most effective approaches to protect crops from virus infection; however, only a few naturally occurring rice genes confer resistance against rice viruses. Many investigators are using genetic engineering of rice plants as a potential strategy to control viral diseases. Using viral genes to confer pathogen-derived resistance against crops is a well-established procedure, and the expression of various viral gene products has proved to be effective in preventing or reducing infection by various plant viruses since the 1990s. RNA interference (RNAi), also known as RNA silencing, is one of the most efficient methods to confer resistance against plant viruses on their respective crops. In this article, we review the recent progress, mainly conducted by our research group, in transgenic strategies to confer resistance against tenuiviruses and reoviruses in rice plants. Our findings also illustrate that not all RNAi constructs against viral RNAs are equally effective in preventing virus infection and that it is important to identify the viral “Achilles’ heel” gene to target for RNAi attack when engineering plants.

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Suppressor of RNA silencing encoded by Rice gall dwarf virus genome segment 11

Cited by 11 publications

References 40 publications

Identification of Pns12 as the second silencing suppressor of Rice gall dwarf virus

Identification of Pns12 as the second silencing suppressor of Rice gall dwarf virus

Expression of rice gall dwarf virus outer coat protein gene (S8) in insect cells

Transgenic strategies to confer resistance against viruses in rice plants

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