Surface characterization, biocompatibility and osteogenic differentiation of drop-casted multilayer graphene oxide film towards human wharton’s jelly derived mesenchymal stem cells

Abstract

Graphene oxide (GO) materials have been extensively employed in mesenchymal stem cell (MSCs) research due to its unique nano topography. Herein, various concentrations of GO flakes were used to fabricate different thickness of multilayer graphene oxide (m-GO) films using a simple drop casting method and characterized by FTIR and AFM. The biocompatibility of m-GO films in culturing WJ-MSCs was investigated based on cell morphology, cell viability and osteogenic differentiation ability. Importantly, WJ-MSCs adhered and proliferated successfully on the m-GO films (6.25 µg, 12.5 µg and 25 µg) and showed no difference in cell morphology and viability after 5 days culture. Moreover, the WJ-MSCs growth on GO films (6.25 µg, 12.5 µg and 25 µg) enhanced the osteogenic differentiation as compared to the control (glass coverslip). Hence, the simple and inexpensive drop-casted fabrication strategy could provide biocompatible m-GO films to unlock the wider potential of WJ-MCSs in tissue engineering application.

Peptide Conjugate on Multilayer Graphene Oxide Film for the Osteogenic Differentiation of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells

“…The preparation process of the peptide conjugate on the multilayer GO (m-GO) films is shown in Scheme 1 . The m-GO films were naturally formed by drying the aqueous solution of GO as previously reported [ 19 , 54 ]. Then, the designated peptide sequences (PepS1, PepS2, PepS3, and PepS4) with positively charged and aromatic rings interacted with the negatively charged m-GO film surface [ 52 , 57 , 58 ].…”

“…The sp2 carbon structure of the GO can be observed at 1729 and 1632 cm −1 , respectively. A broad peak at 3395 cm −1 can be ascribed to the O–H vibrations of the hydroxyl group of the GO [ 54 ]. The FTIR spectra of the m-GO film conjugated with four different peptide sequences (PepS1, PepS2, PepS3, and PepS4) appear to be very similar, exhibiting one broad peak with weaker intensities at ~3390 cm −1 as compared with the m-GO film, which indicates that the O–H vibrations of the hydroxyl group on the surface of the m-GO film were diminished in the presence of the peptide.…”

“…A multilayer graphene oxide (m-GO) film with 25 µg of GO loading prepared according to our previously reported method [ 54 ] was used as a substrate for the fabrication of GO/peptide films. Briefly, 100 µL of the GO flake solution (0.25 mg/mL) was added over the treated 10 mm glass coverslip and allowed to dry at 30 °C for 24 h. GO/peptide films were fabricated following the modified previously reported method [ 52 ].…”

“…In our previous study, we tested the biocompatibility of multilayer GO (m-GO) film toward WJ-MSCs [ 54 ]. The present work aimed to conjugate a peptide on the m-GO film through a self-assembly technique to improve the biocompatibility and osteogenic differentiation of the m-GO film toward WJ-MSCs.…”

Memristive Non-Volatile Memory Based on Graphene Materials

“…The electrochemical exfoliation method reported by Feng, Müllen, and co-workers [1] demonstrated that electrochemically exfoliated graphite provides graphene flakes from one to three layers with a high yield of >80%, a high C/O ratio of~12, a sheet resistance value of 4.8 kΩ•m −1 , and hole mobility of 233 cm 2 •V −1 •s −1 for a single sheet [1]. Currently, solution process methods of graphene and GO materials also receive considerable attention, mainly including spin coating, dip coating, and drop casting [73][74][75][76][77][78]. A comparison of these approaches is shown in Table 2.…”