2009
DOI: 10.1007/s00216-009-2702-3
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Surface-enhanced Raman scattering for protein detection

Abstract: Proteins are essential components of organisms and they participate in every process within cells. The key characteristic of proteins that allows their diverse functions is their ability to bind other molecules specifically and tightly. With the development of proteomics, exploring high-efficiency detection methods for large-scale proteins is increasingly important. In recent years, rapid development of surface-enhanced Raman scattering (SERS)-based biosensors leads to the SERS realm of applications from chemi… Show more

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Cited by 329 publications
(256 citation statements)
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“…Their capability for sensitive and multiplexed protein detection has been demonstrated by Porter and coworkers [7][8][9][10][11] and Ozaki and co-workers. [12][13][14] A typical SERS immunoassay for protein detection is based on the sandwich immunocomplex, in which the capture antibodies are immobilized on a substrate such as glass coated with a thin gold lm [8][9][10] and magnetic beads/nanoparticles. 15,16 The corresponding antigen is captured from the sample solution and detected via the characteristic Raman spectrum of the SERS-labeled detection antibody aer the formation of the sandwich.…”
Section: Introductionmentioning
confidence: 99%
“…Their capability for sensitive and multiplexed protein detection has been demonstrated by Porter and coworkers [7][8][9][10][11] and Ozaki and co-workers. [12][13][14] A typical SERS immunoassay for protein detection is based on the sandwich immunocomplex, in which the capture antibodies are immobilized on a substrate such as glass coated with a thin gold lm [8][9][10] and magnetic beads/nanoparticles. 15,16 The corresponding antigen is captured from the sample solution and detected via the characteristic Raman spectrum of the SERS-labeled detection antibody aer the formation of the sandwich.…”
Section: Introductionmentioning
confidence: 99%
“…Recently, a type of SERS-active substrate with uniformly large and highly reproducible Raman-enhancing power has been developed by growing Ag nanoparticles on arrays of anodic aluminum oxide (AAO) nanochannels to take advantage of the sub-10-nm inter-particle gaps, which act as 'hot junctions' for creating the electromagnetic enhancement 4 . The high sensitivity and reproducibility of such a substrate-hereafter referred to as Ag/AAO-SERS substrate-facilitated the use of SERS for chemical/biological sensing applications [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] . SERS of various types of bacteria including Gram-positive, Gram-negative and mycobacteria have been acquired and the response of bacteria to antibiotics has been examined 21 .…”
mentioning
confidence: 99%
“…by selection of longer wavelength excitations for less fluorescence background productions or using noble metals in SERS to resolve weak signal problems. In addition, SERS optimization has been considered in the detection of biological materials [8,9,1122]. …”
Section: Principles Of Raman Spectroscopymentioning
confidence: 99%
“…Currently available substrates usually contain irregular active sites that suffer from strong spatial and temporal fluctuation in Raman intensity, and thus do not produce stable Raman output [29]. Still, there are a few sample pretreatment methods, such as Western blot SERS, fluorescein isothiocyanate-linked SERS, and ligand SERS, that can increase the sensitivity of SERS detection [22,37]. …”
Section: Principles Of Raman Spectroscopymentioning
confidence: 99%