Surface fluorination of polylactide as a path to improve platelet associated hemocompatibility

Khalifehzadeh, Razieh; Ciridon, Winston; Ratner, Buddy D.

doi:10.1016/j.actbio.2018.07.042

Cited by 29 publications

(22 citation statements)

References 63 publications

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“…Functionalization can not only be executed by coating with cross-linked collagen but also with a fluorination of the material surface to upgrade the actual basic PLA framework e.g., leading to increased hemocompatibility as an indicator of cytocompatibility [33] or elevated cell adhesion [34]. The effect of gas-phase fluorination on biomaterials is not fully understood but includes radical chain reactions on the material surface, resulting in fluorine radical formation, which opens C-H bonds and generates novel C-F, C-F2 and C-F3 groups ( [35], Schroepfer et al: Gas-phase fluorination on PLA improves cell adhesion and spreading, submitted in 2020 to ACS Omega).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Growth of Lapine Anterior Cruciate Ligament-Derived Fibroblasts on Scaffolds Embroidered from Poly(l-lactide-co-ε-caprolactone) and Polylactic Acid Threads Functionalized by Fluorination and Hexamethylene Diisocyanate Cross-Linked Collagen Foams

Gögele

Hahn

Elschner

et al. 2020

IJMS

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Reconstruction of ruptured anterior cruciate ligaments (ACLs) is limited by the availability and donor site morbidity of autografts. Hence, a tissue engineered graft could present an alternative in the future. This study was undertaken to determine the performance of lapine (L) ACL-derived fibroblasts on embroidered poly(l-lactide-co-ε-caprolactone) (P(LA-CL)) and polylactic acid (PLA) scaffolds in regard to a tissue engineering approach for ACL reconstruction. Surface modifications of P(LA-CL)/PLA by gas-phase fluorination and cross-linking of a collagen foam using either ethylcarbodiimide (EDC) or hexamethylene diisocyanate (HMDI) were tested regarding their influence on cell adhesion, growth and gene expression. The experiments were performed using embroidered P(LA-CL)/PLA scaffolds that were seeded dynamically or statically with LACL-derived fibroblasts. Scaffold cytocompatibility, cell survival, numbers, metabolic activity, ultrastructure and sulfated glycosaminoglycan (sGAG) synthesis were evaluated. Quantitative real-time polymerase chain reaction (QPCR) revealed gene expression of collagen type I (COL1A1), decorin (DCN), tenascin C (TNC), Mohawk (MKX) and tenomodulin (TNMD). All tested scaffolds were highly cytocompatible. A significantly higher cellularity and larger scaffold surface areas colonized by cells were detected in HMDI cross-linked and fluorinated scaffolds compared to those cross-linked with EDC or without any functionalization. By contrast, sGAG synthesis was higher in controls. Despite the fact that the significance level was not reached, gene expressions of ligament extracellular matrix components and differentiation markers were generally higher in fluorinated scaffolds with cross-linked collagen foams. LACL-derived fibroblasts maintained their differentiated phenotype on fluorinated scaffolds supplemented with a HMDI cross-linked collagen foam, making them a promising tool for ACL tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Enhanced Growth of Lapine Anterior Cruciate Ligament-Derived Fibroblasts on Scaffolds Embroidered from Poly(l-lactide-co-ε-caprolactone) and Polylactic Acid Threads Functionalized by Fluorination and Hexamethylene Diisocyanate Cross-Linked Collagen Foams

Gögele

Hahn

Elschner

et al. 2020

IJMS

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“…The lower contact angle reflects an increased surface hydrophilicity and higher surface energy. The wettability of material surfaces can influence platelet and cell attachment behavior [33]. As shown in Figure 3B, the water contact angle on SS was 67.8 ± 2.7°.…”

Section: Resultsmentioning

confidence: 99%

Surface-Degradable Drug-Eluting Stent with Anticoagulation, Antiproliferation, and Endothelialization Functions

Hou

Wang

et al. 2019

Biomolecules

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Drug-eluting stents (DES) have been widely applied for saving the life of patients with coronary artery diseases (CADs). However, conventional polymers such as polylactic acid (PLA) and poly (lactic-co-glycolic acid) (PLGA), which are widely applied for drug-eluting stents studies, have serious bulk erosion problems, like high local acidity and poor mechanical properties. Instead, we chose surface erosion polymer poly (1, 3-trimethylene carbonate) (PTMC) as a drug carrier in this study. Here, we fabricated and characterized a novel durable-polymer drug-eluting 316 L stainless steel (SS) stent, in which the inner surface was coated with a Ti–O film using the magnetron sputtering method to promote the growth of endothelial cells (ECs). On the outer layer of the stent, first, a Ti–O film was deposited and, then, on top of it a rapamycin-loaded PTMC coat was deposited using the ultrasonic atomization spray method. This dual coating inhibited the migration and expansion of smooth muscle cells (SMCs). The drug coating also inhibited the adhesion/activation of platelets. In tests on dogs, it was found the novel stent promoted re-endothelialization and reduced restenosis, in contrast to the plain SS stent. Thus, the novel stent may have promise for use in treating patients with CAD.

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“…the fluorinated surface shows relatively few adherent platelets, and those platelets are less activated. In terms of reducing thrombogenicity and reducing platelet reactivity, these fluorinated surfaces will show improved blood compatibility in the body [14] . The use of poly (ethylene glycol) (PEG) to modify PLA-based nanocarriers is a strategy often used in research.…”

Section: Hemocompatibility Characteristics or Improvement Strategies Of Various Nanocarriersmentioning

confidence: 99%

Relationship and improvement strategies between drug nanocarrier characteristics and hemocompatibility: What can we learn from the literature

Guo

Shi

et al. 2021

Asian Journal of Pharmaceutical Sciences

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This article discusses the various blood interactions that may occur with various types of nano drug-loading systems. Nanoparticles enter the blood circulation as foreign objects. On the one hand, they may cause a series of inflammatory reactions and immune reactions, resulting in the rapid elimination of immune cells and the reticuloendothelial system, affecting their durability in the blood circulation. On the other hand, the premise of the drug-carrying system to play a therapeutic role depends on whether they cause coagulation and platelet activation, the absence of hemolysis and the elimination of immune cells. For different forms of nano drug-carrying systems, we can find the characteristics, elements and coping strategies of adverse blood reactions that we can find in previous researches. These adverse reactions may include destruction of blood cells, abnormal coagulation system, abnormal effects of plasma proteins, abnormal blood cell behavior, adverse immune and inflammatory reactions, and excessive vascular stimulation. In order to provide help for future research and formulation work on the blood compatibility of nano drug carriers.

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Surface fluorination of polylactide as a path to improve platelet associated hemocompatibility

Cited by 29 publications

References 63 publications

Enhanced Growth of Lapine Anterior Cruciate Ligament-Derived Fibroblasts on Scaffolds Embroidered from Poly(l-lactide-co-ε-caprolactone) and Polylactic Acid Threads Functionalized by Fluorination and Hexamethylene Diisocyanate Cross-Linked Collagen Foams

Enhanced Growth of Lapine Anterior Cruciate Ligament-Derived Fibroblasts on Scaffolds Embroidered from Poly(l-lactide-co-ε-caprolactone) and Polylactic Acid Threads Functionalized by Fluorination and Hexamethylene Diisocyanate Cross-Linked Collagen Foams

Surface-Degradable Drug-Eluting Stent with Anticoagulation, Antiproliferation, and Endothelialization Functions

Relationship and improvement strategies between drug nanocarrier characteristics and hemocompatibility: What can we learn from the literature

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