Surface Minimal Bactericidal Concentration: A comparative study of active glasses functionalized with different-sized silver nanoparticles

Barzan, Giulia; Rocchetti, Luca; Portesi, C.; Pellegrino, Francesco; Taglietti, Angelo; Rossi, Andrea Mario; Giovannozzi, Andrea Mario

doi:10.1016/j.colsurfb.2021.111800

Cited by 14 publications

(10 citation statements)

References 49 publications

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“…By cautiously altering the nano-structures, their solubility, biocompatibility, stability and molecule binding ability could be enhanced. The development of hybrid systems made up of nanoparticles and biological molecules opens the door to a wide range of applications, such as biosensing, bioimaging and targeted drug delivery [11][12][13][14][15][16]. In addition, hybrid systems have been proved to improve oral delivery by increasing the bioavailability of the drugs that are not well absorbed [17].…”

Section: Introductionmentioning

confidence: 99%

Delafloxacin-Capped Gold Nanoparticles (DFX-AuNPs): An Effective Antibacterial Nano-Formulation of Fluoroquinolone Antibiotic

et al. 2022

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New antibiotics are seen as ‘drugs of last resort’ against virulent bacteria. However, development of resistance towards new antibiotics with time is a universal fact. Delafloxacin (DFX) is a new fluoroquinolone antibiotic that differs from existing fluoroquinolones by the lack of a protonatable substituent, which gives the molecule a weakly acidic nature, affording it higher antibacterial activity under an acidic environment. Furthermore, antibiotic-functionalized metallic nanoparticles have been recently emerged as a feasible platform for conquering bacterial resistance. In the present study, therefore, we aimed at preparing DFX-gold nano-formulations to increase the antibacterial potential of DFX. To synthesize DFX-capped gold nanoparticles (DFX-AuNPs), DFX was used as a reducing and stabilizing/encapsulating agent. Various analytical techniques such as UV-visible spectroscopy, TEM, DLS, FTIR and zeta potential analysis were applied to determine the properties of the synthesized DFX-AuNPs. The synthesized DFX-AuNPs revealed a distinct surface plasmon resonance (SPR) band at 530 nm and an average size of 16 nm as manifested by TEM analysis. In addition, Zeta potential results (−19 mV) confirmed the stability of the synthesized DFX-AuNPs. Furthermore, FTIR analysis demonstrated that DFX was adsorbed onto the surface of AuNPs via strong interaction between AuNPs and DFX. Most importantly, comparative antibacterial analysis of DFX alone and DFX-AuNPs against Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus and Bacillus subtilis) verified the superior antibacterial activity of DFX-AuNPs against the tested microorganisms. To sum up, DFX gold nano-formulations can offer a promising possible solution, even at a lower antibiotic dose, to combat pathogenic bacteria.

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Section: Introductionmentioning

confidence: 99%

Delafloxacin-Capped Gold Nanoparticles (DFX-AuNPs): An Effective Antibacterial Nano-Formulation of Fluoroquinolone Antibiotic

et al. 2022

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“…The minimum inhibitory concentration (MIC) is the minimum concentration of an antimicrobial agent that prevents the turbidity caused by the growth of bacteria in the liquid microbial culture medium. 88 The standard broth dilution method was used to evaluate the antimicrobial effect of the prepared CFM@SiO 2 /PVA/Au− CR TNBC by evaluating the observable growth of microorganisms in the agar broth. One-day culture of E. coli and K. pneumoniae broth was prepared at 37 °C.…”

Section: Resultsmentioning

confidence: 99%

Cefixime-Containing Silica Nanoseeds Coated by a Hybrid PVA-Gold Network with a Cys–Arg Dipeptide Conjugation: Enhanced Antimicrobial and Drug Release Properties

et al. 2021

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Therapeutic nano-bioconjugates (TNBCs) as an advanced class of drug delivery systems have attracted much attention due to more efficacy than the individual medications. Hence, in this study, a novel anti-infection TNBC system is designed based on highly porous silica nanoparticles, gold nanoparticles (AuNPs), and hybridized polyvinyl alcohol (PVA) for the efficient delivery of cefixime (CFM). Furthermore, a conjugation of cysteine−arginine (CR) dipeptide is made onto the surfaces for the enhancement of cell adhesion. Concisely, the AuNPs incorporated inside the PVA network play the key role in the controlled release process triggered by localized surface plasmon resonance (LSPR) heating. The drug content of the CFM-containing cargo (named as CFM@SiO 2 /PVA/Au−CR) and related release profile have been precisely studied by the confirmed analytical methods. Eventually, confocal microscopy on the stained cells has revealed that the TNBC particles are capable of entering the Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) bacterial cells better than the individual CFM. Also, optical density experiments (OD 600 ) have corroborated that the prepared CFM@SiO 2 /PVA/Au−CR TNBC includes a high antimicrobial effect on K. pneumoniae and E. coli cells with (93.0 ± 1.5) % and (86.8 ± 1.0) % success rates, respectively, whereas the same dosage of the individual CFM has shown a lower effect on the cell growth rate. Also, estimation of minimum inhibitory/ bactericidal concentrations (MIC/MBC) confirmed the enhanced antibacterial property of the CFM through the presented delivery method. Overall, this product is suggested to be clinically administrated instead of the individual CFM due to its high efficacy and containing lower dosage of the antibiotic drug.

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“…However, García et al [70] have reported that at high inoculum levels, cells exhibit reduced absorption of disinfectants, yet the underlying mechanism behind this phenomenon remains relatively unknown and understudied [70]. From the literature reviewed, it appears that changes in the density of the test inoculum are not frequent, with only three authors [34,52,55] reporting the use of higher inoculum densities, namely, Barzan et al [52], Richert et al, [55] and Yamada et al [34], who opted for 1.0 × 10 6 CFU/mL, 10 6 CFU/mL and 0.4 to 3.0 × 10 8 CFU/mL, respectively. These authors provided no comments on whether this change in densities significantly affected the antibacterial activity efficacy of the compounds tested.…”

Section: Inoculummentioning

confidence: 99%

“…These authors provided no comments on whether this change in densities significantly affected the antibacterial activity efficacy of the compounds tested. Conversely, 37% of the authors (20 studies) [2,8,24,27,31,32,[38][39][40][41]46,47,50,52,54,56,59,66,68] reported an adjustment of the bacterial inoculum volume due to the size of the test specimen used. This modification has not been reported to influence the result of the protocol since ISO 22196 allows for different sizes/measurements of the test specimen and plastic cover film, as long as the inoculum volume is adjusted to be proportional to the area of the cover film used.…”

Section: Inoculummentioning

confidence: 99%

Assessing Antimicrobial Efficacy on Plastics and Other Non-Porous Surfaces: A Closer Look at Studies Using the ISO 22196:2011 Standard

Bento de Carvalho,

Barbosa,

Teixeira

2024

Biology

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The survival and spread of foodborne and nosocomial-associated bacteria through high-touch surfaces or contamination-prone sites, in either healthcare, domestic or food industry settings, are not always prevented by the employment of sanitary hygiene protocols. Antimicrobial surface coatings have emerged as a solution to eradicate pathogenic bacteria and prevent future infections and even outbreaks. Standardised antimicrobial testing methods play a crucial role in validating the effectiveness of these materials and enabling their application in real-life settings, providing reliable results that allow for comparison between antimicrobial surfaces while assuring end-use product safety. This review provides an insight into the studies using ISO 22196, which is considered the gold standard for antimicrobial surface coatings and examines the current state of the art in antimicrobial testing methods. It primarily focuses on identifying pitfalls and how even small variations in methods can lead to different results, affecting the assessment of the antimicrobial activity of a particular product.

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Surface Minimal Bactericidal Concentration: A comparative study of active glasses functionalized with different-sized silver nanoparticles

Cited by 14 publications

References 49 publications

Delafloxacin-Capped Gold Nanoparticles (DFX-AuNPs): An Effective Antibacterial Nano-Formulation of Fluoroquinolone Antibiotic

Delafloxacin-Capped Gold Nanoparticles (DFX-AuNPs): An Effective Antibacterial Nano-Formulation of Fluoroquinolone Antibiotic

Cefixime-Containing Silica Nanoseeds Coated by a Hybrid PVA-Gold Network with a Cys–Arg Dipeptide Conjugation: Enhanced Antimicrobial and Drug Release Properties

Assessing Antimicrobial Efficacy on Plastics and Other Non-Porous Surfaces: A Closer Look at Studies Using the ISO 22196:2011 Standard

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