Surface Modification of Polycarbonate Urethane with Zwitterionic Polynorbornene via Thiol‐ene Click‐Reaction to Facilitate Cell Growth and Proliferation

Abstract

Herein, we grafted the zwitterionic polynorbornene onto polycarbonate urethane (PCU) film surface by a convenient route of thiol-ene click-chemistry. The PCU film surface was first treated with hexamethylene-1,6-diisocynate and subsequently with two different thiol agents (L-cysteine and b-marcaptoethanol) in the presence of di-n-butyltin dilaurate (DBTDL) to immobilize sulfhydryl groups onto the surface. Here, DBTDL acted as selective catalyst for the reaction between surface-tethered isocyanates and amine/hydroxyl groups in thiol agents over that of free thiol groups. In the next step, zwitterionic polynorbornene (poly(NSulfoZI)) having functionalizable double bonds was grafted onto these surfaces by photo-initiated thiol-ene click-reaction. The modified surfaces were characterized by water contact angle and XPS analysis. Moreover, the cytocompatibility of these surfaces was investigated by model endothelial cells, EA.hy926, for 1, 3, and 7 d culture times, which showed enhanced cell adhesion and growth. Therefore, the poly(NSulfoZI) functionalized PCU surface using l-cysteine as thiol agent could be a good candidate for tissue engineering material application.

Biofunctionalized Electrospun PCL‐PIBMD/SF Vascular Grafts with PEG and Cell‐Adhesive Peptides for Endothelialization

“…The first approach is to improve the hemocompatibility of the internal lumen of the grafts with some antithrombogenic treatments or agents. Hydrophilic polymers, zwitterionic polymers, and antithrombogenic drugs have been widely used to improve the hemocompatibility of biomaterial surface . Polyethylene glycol (PEG) and other hydrophilic polymers can inhibit platelet adhesion and nonspecific protein adsorption, thus enhancing hemocompatibility.…”

REDV–polyethyleneimine complexes for selectively enhancing gene delivery in endothelial cells

“…Various bioinert and bioactive molecules such as poly(ethylene glycol) (PEG), zwitterionic polymers, heparin, silk-fibroin and gelatin have been used to improve the hemocompatibility of scaffolds. [2][3][4][5][6][7][8][9][10][11] The modification can improve the hydrophilicity and reduce their interaction with proteins and platelets, thus significantly enhancing the hemocompatibility. [12][13][14][15] In order to prevent thrombosis and restenosis in implants, rapid endothelialization which can form a robust and confluent endothelial cell (EC) monolayer has been considered to be another promising method.…”

Targeting REDV peptide functionalized polycationic gene carrier for enhancing the transfection and migration capability of human endothelial cells

“…We have modified artificial vascular grafts by poly(ethylene glycol), gelatin, silk fibroin, and zwitterionic polymers with the aim of minimizing protein adsorption and hindering thrombocyte adhesion. [7][8][9][10][11][12][13][14][15] Besides poor hemocompatibility, the delayed or uncompleted endothelialization on the artificial vascular grafts is another major reason for the occurrence of restenosis and thrombosis. Thus, rapid and complete endothelialization of vascular grafts is a radical approach to avoid a series of pathological processes.…”