Surface Plasmon Resonance Microscopy Based on Total Internal Reflection

Abstract

Surface plasmon resonance microscopy (SPRM) has been widely employed in biological fields because of its high spatial resolution and label-free detection modality. In this study, SPRM based on total internal reflection (TIR) is studied via a home-built SPRM system, and the principle of imaging of a single nanoparticle is analyzed as well. By designing a ring filter and combining it with the deconvolution algorithm in Fourier space, the parabolic tail of the nanoparticle image is removed, in which a spatial resolution of 248 nm is obtained. In addition, we also measured the specific binding between the human IgG antigen and goat anti-human IgG antibody using the TIR-based SPRM. The experimental results have proved that the system can image sparse nanoparticles and monitor biomolecular interactions.

Reconstruction Filters Improving the Spatial Resolution and Signal-to-Noise Ratio of Surface Plasmon Resonance Microscopy

“…Compared with previous works, ,, our work has three improvements. First, all the researchers’ work focused on reconstruction to small nanoparticles, i.e., 100 nm PS nanospheres, exosomes, and 200 nm PS nanospheres, to manifest the improvement in spatial resolution.…”

“…Compared with previous works, 9,24,25 our work has three improvements. First, all the researchers' work focused on reconstruction to small nanoparticles, i.e., 100 nm PS nanospheres, 24 exosomes, 9 and 200 nm PS nanospheres, 25 to manifest the improvement in spatial resolution. Our work discussed the reconstruction of particles with different sizes, materials, and shapes, which extends the reconstruction of SPRM to a universal method.…”

“…9 Recently, Zhang et al reconstructed the SPRM imaging of 200 nm PS nanospheres with improved spatial resolution of 248 nm using a ring shape filter. 25 The scattered SPP retrieval from k-space has demonstrated effective reconstruction to SPRM imaging of nanospheres with diameters no larger than 200 nm to illustrate the improved spatial resolution. For nanoparticles with different sizes and shapes, scattered SPPs indicate different distribution features.…”

“…Also, Yang et al reconstructed the SPRM imaging of single exosomes and improved the spatial resolution to 260 nm . Recently, Zhang et al reconstructed the SPRM imaging of 200 nm PS nanospheres with improved spatial resolution of 248 nm using a ring shape filter . The scattered SPP retrieval from k -space has demonstrated effective reconstruction to SPRM imaging of nanospheres with diameters no larger than 200 nm to illustrate the improved spatial resolution.…”