Surface potential and roughness controlled cell adhesion and collagen formation in electrospun PCL fibers for bone regeneration

Metwally, Sara; Ferraris, Sara; Spriano, Silvia Maria; Krysiak, Zuzanna J.; Kaniuk, Łukasz; Marzec, Mateusz; Kim, Sung Kyun; Szewczyk, Piotr K.; Gruszczyński, Adam; Wytrwał-Sarna, Magdalena; Karbowniczek, Joanna; Bernasik, Andrzej; Kar‐Narayan, Sohini; Stachewicz, Urszula

doi:10.1016/j.matdes.2020.108915

Cited by 139 publications

(98 citation statements)

References 39 publications

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“…The greatest spreading area was observed for sPCL-aligned fibers due to its low interfiber porosity (see Figure 2 B) that inhibited its penetration deeper into the patch, and oil spreading was limited to the fibers top surface layer ( Figure 5 D–F). The increased spreading area on pPCL compared to sPCL random fibers was due to the change in wetting properties of smooth and porous PCL fibers [ 9 ]. Patches with reduced oil spreading areas showed higher oil release in time, as the oil penetrated inside the 3D patch system.…”

Section: Discussionmentioning

confidence: 99%

“…In the second sub-type, the drug is dissolved or dispersed in an adhesive layer which is in contact with the skin [ 6 ]. Fibrous membranes, scaffolds, and patches have been widely utilized in tissue engineering [ 7 , 8 , 9 ], hemostats [ 10 ], tendon repair [ 11 ], drug delivery systems for wound healing [ 12 , 13 ], and in the face masks applications [ 14 , 15 ]. Importantly, the scaffold must show adequate mechanical properties, to provide robust support to the damaged tissue.…”

Section: Introductionmentioning

confidence: 99%

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Electrospun PCL Patches with Controlled Fiber Morphology and Mechanical Performance for Skin Moisturization via Long-Term Release of Hemp Oil for Atopic Dermatitis

et al. 2020

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Atopic dermatitis (AD) is a chronic, inflammatory skin condition, caused by wide genetic, environmental, or immunologic factors. AD is very common in children but can occur at any age. The lack of long-term treatments forces the development of new strategies for skin regeneration. Polycaprolactone (PCL) is a well-developed, tissue-compatible biomaterial showing also good mechanical properties. In our study, we designed the electrospun PCL patches with controlled architecture and topography for long-term release in time. Hemp oil shows anti-inflammatory and antibacterial properties, increasing also the skin moisture without clogging the pores. It can be used as an alternative cure for patients that do not respond to traditional treatments. In the study, we tested the mechanical properties of PCL fibers, and the hemp oil spreading together with the release in time measured on skin model and human skin. The PCL membranes are suitable material as patches or bandages, characterized by good mechanical properties and high permeability. Importantly, PCL patches showed release of hemp oil up to 55% within 6 h, increasing also the skin moisture up to 25%. Our results confirmed that electrospun PCL patches are great material as oil carriers indicating a high potential to be used as skin patches for AD skin treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Electrospun PCL Patches with Controlled Fiber Morphology and Mechanical Performance for Skin Moisturization via Long-Term Release of Hemp Oil for Atopic Dermatitis

et al. 2020

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“…The morphology and size of fibers prepared via electrospinning were affected by the polymer solution, environmental conditions, and applied electric field ( Krysiak et al, 2020 ; Szewczyk et al, 2020 ). It was followed by mechanical ( Ura et al, 2020 ) and surface properties ( Metwally et al, 2020 ). Therefore, PHBV-based scaffolds described in Suslu et al (2014) had significantly smaller diameters of 571 ± 160 nm compared to our study, however, similarly, they obtained increased fiber diameter with the addition of HA particles.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Cells Anchoring to Electrospun Hybrid Scaffolds With PHBV and HA Particles for Bone Tissue Regeneration

Karbowniczek

Kaniuk

Berniak

et al. 2021

Front. Bioeng. Biotechnol.

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Hybrid materials combining organic and inorganic compounds used as scaffolds are highly beneficial in bone regeneration. In this study, we successfully produced by blend electrospinning poly(3-hydroxybutyric acid-co-3-hydrovaleric acid) (PHBV) scaffolds enriched with hydroxyapatite (HA) particles to biomimic bone tissue for improved and faster regeneration processes. The morphology, fiber diameters, and composition of the scaffolds were investigated by scanning electron microscopy (SEM) techniques followed by focused ion beam (FIB) sectioning to verify HA particles integration with PHBV fibers. In vitro cell culture was performed for 7 days and followed with the cell proliferation test (CellTiter-Blue® Assay). Additionally, cell integration with the scaffold was visualized by confocal and SEM imaging. We developed a simple way of obtaining hybrid scaffolds by electrospinning PHBV solution with HA particles without any post-processing. The PHBV + HA scaffold enhanced cell proliferation and filopodia formation responsible for cell anchoring within the created 3D environment. The obtained results show the great potential in the development of hybrid scaffolds stimulating bone tissue regeneration.

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“…Accordingly, Guarino et al demonstrated that the use of highly polar solvents coupled with low concentration of the PCL solution tends to form more crystalline fibers rather than high concentrated solutions and low polar/apolar solvents. Other studies also suggested a further contribution of polymer crystallinity on cellular response mediated by changes in surface roughness at the nanometric scale [77,78], which is universally recognized to play a crucial role on the interface between cells and surrounding material.…”

Section: Natural Vs Synthetic Fibersmentioning

confidence: 98%

Topographical and Biomechanical Guidance of Electrospun Fibers for Biomedical Applications

et al. 2020

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Electrospinning is gaining increasing interest in the biomedical field as an eco-friendly and economic technique for production of random and oriented polymeric fibers. The aim of this review was to give an overview of electrospinning potentialities in the production of fibers for biomedical applications with a focus on the possibility to combine biomechanical and topographical stimuli. In fact, selection of the polymer and the eventual surface modification of the fibers allow selection of the proper chemical/biological signal to be administered to the cells. Moreover, a proper design of fiber orientation, dimension, and topography can give the opportunity to drive cell growth also from a spatial standpoint. At this purpose, the review contains a first introduction on potentialities of electrospinning for the obtainment of random and oriented fibers both with synthetic and natural polymers. The biological phenomena which can be guided and promoted by fibers composition and topography are in depth investigated and discussed in the second section of the paper. Finally, the recent strategies developed in the scientific community for the realization of electrospun fibers and for their surface modification for biomedical application are presented and discussed in the last section.

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Surface potential and roughness controlled cell adhesion and collagen formation in electrospun PCL fibers for bone regeneration

Cited by 139 publications

References 39 publications

Electrospun PCL Patches with Controlled Fiber Morphology and Mechanical Performance for Skin Moisturization via Long-Term Release of Hemp Oil for Atopic Dermatitis

Electrospun PCL Patches with Controlled Fiber Morphology and Mechanical Performance for Skin Moisturization via Long-Term Release of Hemp Oil for Atopic Dermatitis

Enhanced Cells Anchoring to Electrospun Hybrid Scaffolds With PHBV and HA Particles for Bone Tissue Regeneration

Topographical and Biomechanical Guidance of Electrospun Fibers for Biomedical Applications

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