Surface Properties and Osteoblastic Cytocompatibility of Two Blasted and Acid-Etched Titanium Implant Systems with Distinct Microtopography

Mesquita, Pedro; Gomes, Pedro; Sampaio, Paula; Juodžbalys, Gintaras; Afonso, Américo; Fernandes, Maria Helena

doi:10.5037/jomr.2012.3104

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…Tissue scaffold morphology and cell-biomaterial interaction sets the stage for cell attachment and also affects the cell phenotype and functions [14,35,36]. Therefore, it is important to understand the effect of PCL nanofiber introduction within collagen scaffolds in respect to scaffold morphology.…”

Section: Pcl Nanofiber Concentration Within Pn-col Mediates Scaffold ...mentioning

confidence: 99%

Polycaprolactone nanofiber interspersed collagen type-I scaffold for bone regeneration: a unique injectable osteogenic scaffold

Baylan¹,

Bhat²,

Ditto³

et al. 2013

Biomed. Mater.

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There is an increasing demand for an injectable cell coupled three-dimensional (3D) scaffold to be used as bone fracture augmentation material. To address this demand, a novel injectable osteogenic scaffold called PN-COL was developed using cells, a natural polymer (collagen type-I), and a synthetic polymer (polycaprolactone (PCL)). The injectable nanofibrous PN-COL is created by interspersing PCL nanofibers within pre-osteoblast cell embedded collagen type-I. This simple yet novel and powerful approach provides a great benefit as an injectable bone scaffold over other non-living bone fracture stabilization polymers, such as polymethylmethacrylate and calcium content resin-based materials. The advantages of injectability and the biomimicry of collagen was coupled with the structural support of PCL nanofibers, to create cell encapsulated injectable 3D bone scaffolds with intricate porous internal architecture and high osteoconductivity. The effects of PCL nanofiber inclusion within the cell encapsulated collagen matrix has been evaluated for scaffold size retention and osteocompatibility, as well as for MC3T3-E1 cells osteogenic activity. The structural analysis of novel bioactive material proved that the material is chemically stable enough in an aqueous solution for an extended period of time without using crosslinking reagents, but it is also viscous enough to be injected through a syringe needle. Data from long-term in vitro proliferation and differentiation data suggests that novel PN-COL scaffolds promote the osteoblast proliferation, phenotype expression, and formation of mineralized matrix. This study demonstrates for the first time the feasibility of creating a structurally competent, injectable, cell embedded bone tissue scaffold. Furthermore, the results demonstrate the advantages of mimicking the hierarchical architecture of native bone with nano- and micro-size formation through introducing PCL nanofibers within macron-size collagen fibers and in promoting osteoblast phenotype progression for bone regeneration.

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Section: Pcl Nanofiber Concentration Within Pn-col Mediates Scaffold ...mentioning

confidence: 99%

Polycaprolactone nanofiber interspersed collagen type-I scaffold for bone regeneration: a unique injectable osteogenic scaffold

Baylan¹,

Bhat²,

Ditto³

et al. 2013

Biomed. Mater.

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show abstract

“…In addition, the micro-roughness of titanium alloys has also been reported [17]. Numerous studies have shown that such micro-scale topography promotes the adhesion, differentiation, and extracellular matrix formation and mineralization of osteoblasts [18][19][20][21][22]. In contrast, several studies have shown that such osteoblast attachment, spreading, and proliferation can be negatively affected by micro-scale topography [19,20,23].…”

Section: Introductionmentioning

confidence: 99%

A Newly Created Meso-, Micro-, and Nano-Scale Rough Titanium Surface Promotes Bone-Implant Integration

Hasegawa

Saruta

Hirota

et al. 2020

IJMS

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Titanium implants are the standard therapeutic option when restoring missing teeth and reconstructing fractured and/or diseased bone. However, in the 30 years since the advent of micro-rough surfaces, titanium’s ability to integrate with bone has not improved significantly. We developed a method to create a unique titanium surface with distinct roughness features at meso-, micro-, and nano-scales. We sought to determine the biological ability of the surface and optimize it for better osseointegration. Commercially pure titanium was acid-etched with sulfuric acid at different temperatures (120, 130, 140, and 150 °C). Although only the typical micro-scale compartmental structure was formed during acid-etching at 120 and 130 °C, meso-scale spikes (20–50 μm wide) and nano-scale polymorphic structures as well as micro-scale compartmental structures formed exclusively at 140 and 150 °C. The average surface roughness (Ra) of the three-scale rough surface was 6–12 times greater than that with micro-roughness only, and did not compromise the initial attachment and spreading of osteoblasts despite its considerably increased surface roughness. The new surface promoted osteoblast differentiation and in vivo osseointegration significantly; regression analysis between osteoconductivity and surface variables revealed these effects were highly correlated with the size and density of meso-scale spikes. The overall strength of osseointegration was the greatest when the acid-etching was performed at 140 °C. Thus, we demonstrated that our meso-, micro-, and nano-scale rough titanium surface generates substantially increased osteoconductive and osseointegrative ability over the well-established micro-rough titanium surface. This novel surface is expected to be utilized in dental and various types of orthopedic surgical implants, as well as titanium-based bone engineering scaffolds.

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“…It is well known that cell behaviors are strongly influenced by topographical features. [21][22][23] First, grain size plays an important role in the improved biocompatibility of USP-Ti. Cells are willing to respond to nanostructure surfaces, since in vivo they live inside an extracellular matrix containing nanoscale collagen fibrils and since their own surfaces are structured on the nanoscale level (receptors and filopodia).…”

mentioning

confidence: 99%

Increased osteoblast function in vitro and in vivo through surface nanostructuring by ultrasonic shot peening

Guo

Tang

et al. 2015

IJN

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Surface topography has significant influence on good and fast osseointegration of biomedical implants. In this work, ultrasonic shot peening was conducted to modify titanium to produce nanograined (NG) surface. Its ability to induce new bone formation was evaluated using an in vivo animal model. We demonstrated that the NG surface enhanced osteoblast adhesion, proliferation, differentiation, and mineralization in in vitro experiments compared to coarse-grained titanium surface. Push-out test, histological observations, fluorescent labeling, and histomorphometrical analysis consistently indicated that the NG surfaces developed have the higher osseointegration than coarse-grained surfaces. Those results suggest that ultrasonic shot peening has the potential for future use as a surface modification method in biomedical application.

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Surface Properties and Osteoblastic Cytocompatibility of Two Blasted and Acid-Etched Titanium Implant Systems with Distinct Microtopography

Cited by 6 publications

References 25 publications

Polycaprolactone nanofiber interspersed collagen type-I scaffold for bone regeneration: a unique injectable osteogenic scaffold

Polycaprolactone nanofiber interspersed collagen type-I scaffold for bone regeneration: a unique injectable osteogenic scaffold

A Newly Created Meso-, Micro-, and Nano-Scale Rough Titanium Surface Promotes Bone-Implant Integration

Increased osteoblast function in vitro and in vivo through surface nanostructuring by ultrasonic shot peening

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