Surface-textured PEG-based hydrogels with adjustable elasticity: Synthesis and characterization

Pfister, Pascal; Wendlandt, Michael; Neuenschwander, Peter; Suter, Ulrich W.

doi:10.1016/j.biomaterials.2006.09.016

Cited by 36 publications

(30 citation statements)

References 57 publications

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“…In addition, the mechanical properties of PEG acrylates can be tuned by varying PEG molecular weight, monomer concentration, and crosslinking density. 12,44,45 Previous studies have also focused on the effects of 2D substrate stiffness or 3D homogeneous matrix properties. [46][47][48] In contrast, this PEGDMA microrods system was developed to investigate the effect of micromechanical domains on cell proliferation and gene expression in 3D.…”

Section: Discussionmentioning

confidence: 99%

Microtopographical Cues in 3D Attenuate Fibrotic Phenotype and Extracellular Matrix Deposition: Implications for Tissue Regeneration

Ayala

López

Desai

2010

Tissue Engineering Part A

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Recent studies have highlighted the role of external biophysical cues on cell morphology and function. In particular, substrate geometry and rigidity in two dimensions has been shown to impact cell growth, death, differentiation, and motility. Knowledge of how these physical cues affect cell function in three dimensions is critical for successful development of novel regenerative therapies. In this work, the effect of discrete micromechanical cues in three-dimensional (3D) system on cell proliferation, gene expression, and extracellular matrix synthesis was investigated. Poly(ethylene glycol) dimethacrylate hydrogel microrods were fabricated using photolithography and suspended in gel to create a 3D culture with microscale cues of defined mechanical properties in the physiological range (2-50 kPa). These microrods significantly affected fibroblast proliferation, matrix production, and gene expression. Cultures with stiff microrods reduced fibroblast proliferation and downregulated expression of key extracellular matrix proteins involved in scar tissue formation. In addition, the contractility marker alpha smooth muscle actin and adhesion molecule integrin a3 were also significantly downregulated. Cultures with soft microrods had no significant difference on fibroblast proliferation and expression of Cyclin D1, alpha smooth muscle actin, and integrin a3 compared to cultures with no microrods. Here, we present a new platform of potentially injectable microrods with tunable elasticity; in addition, we show that cell proliferation and gene expression are influenced by discrete physical cues in 3D.

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Section: Discussionmentioning

confidence: 99%

Microtopographical Cues in 3D Attenuate Fibrotic Phenotype and Extracellular Matrix Deposition: Implications for Tissue Regeneration

Ayala

López

Desai

2010

Tissue Engineering Part A

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“…Additionally, PEGDA-based products have also been successfully used for injectable drug delivery systems [24][25][26] and cell culture mediums. [27][28][29] Although the composite systems of PEGDA and other materials have been extensively developed and used, few studies directly addressed the effects of PEGDA molecular weights on the properties of hygrogels with same polymer concentration.…”

mentioning

confidence: 99%

Controllable properties and microstructure of hydrogels based on crosslinked poly(ethylene glycol) diacrylates with different molecular weights

Zhang

Wang

Song

et al. 2011

J of Applied Polymer Sci

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This work describes a comprehensive study of hydrogels based on polyethylene glycol diacrylates (PEGDAs) with the molecular weight (MW) range of 400-2000. The blends of low-and high-molecular weight PEGDA macromers with different ratios were photopolymerized under visible light irradiation, using a blue light sensitive photoinitiator Irgacure819, at the total polymer concentration of 60 wt %. Swelling ratios, wetting property, elastic moduli, transparency, and the microstructure of the resulting hydrogels were investigated. Among them, equilibrium water contents, hydrophilicity, and mesh size of the hydrogels increased while the elastic moduli decreased when increased the PEGDA MW or the content of higher MW PEGDA in the blends. Most of the hydrogels possessed excellent transparency in visible region. The viability of L929 cells on the surface of hydrogel was also estimated. All the selected hydrogels exhibited a relatively high proliferation rate, which demonstrated this hydrogel system with photoinitiator Irgacure819 had good biocompatibility. These results show the properties of PEGDA hydrogel could be easily adjusted by varying PEGDA MW or the ratios of low-and high-MW macromers in the composites. It could be helpful for the design of proper PEGDA hydrogels in the applications as tissue engineering or drug delivery system.

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“…Pluronic is also formulated into hydrogels with relatively weak mechanical properties (maximum shear storage modulus of 13.7 kPa at 20 wt% pluronic) used for drug delivery [278] and soft tissue engineering [279]. The mechanical strength of these polyether-based hydrogels is enhanced through cross-linking with excess acryloyl chloride or methacryloyl chloride yielding to the hydrogels PEG diacrylate (PEGDA; shear storage modulus of 68 kPa at 20 wt%) [280] and PEG dimethacrylate (PEGDMA; shear storage modulus of 125 kPa at 20 wt%) [281] that are used in the delivery of chemotherapeutics, hormones, antibacterials, and anti-inflammatory drugs [282] as well as scaffolds in the engineering of cartilage, bone, endothelial, and vascular tissues [283]. PEGDA and PEGDMA are also available in composite systems with other acrylated and methacrylated degradable polymers, most commonly polyesters [284], polyanhydrides [285], and chitosan [286].…”

Section: Synthetic Polyethersmentioning

confidence: 99%

Biodegradable polymers for dental tissue engineering and regeneration

Conte¹,

Riccitiello²,

Luise³

et al. 2017

Biodegradable Polymers: Recent Developments and New Perspectives

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Surface-textured PEG-based hydrogels with adjustable elasticity: Synthesis and characterization

Cited by 36 publications

References 57 publications

Microtopographical Cues in 3D Attenuate Fibrotic Phenotype and Extracellular Matrix Deposition: Implications for Tissue Regeneration

Microtopographical Cues in 3D Attenuate Fibrotic Phenotype and Extracellular Matrix Deposition: Implications for Tissue Regeneration

Controllable properties and microstructure of hydrogels based on crosslinked poly(ethylene glycol) diacrylates with different molecular weights

Biodegradable polymers for dental tissue engineering and regeneration

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