Surveillance of Enteric Viruses and Thermotolerant Coliforms in Surface Water and Bivalves from a Mangrove Estuary in Southeastern Brazil

Keller, Regina; Pratte-Santos, Rodrigo; Scarpati, Karolina; Martins, Sara Angelino; Loss, Suzanne Mariane; Fumian, Túlio Machado; Miagostovich, Marize Pereira; Cassini, Sérvio Túlio Alves

doi:10.1007/s12560-019-09391-3

Cited by 22 publications

(15 citation statements)

References 55 publications

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“…In Brazil, there is no specific legislation to evaluate these pathogens in food; however, there are environmental monitoring studies for the occurrence of enteric viruses [ 24 , 36 , 37 ]. The presence of these pathogens in the samples evaluated in the present study demonstrates poor hygiene conditions in places where products are handled and marketed; however, it does not allow for inferring their infectious capacities.…”

Section: Discussionmentioning

confidence: 99%

Detection of adenovirus, rotavirus, and hepatitis E virus in meat cuts marketed in Uruguaiana, Rio Grande do Sul, Brazil

et al. 2022

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Section: Discussionmentioning

confidence: 99%

Detection of adenovirus, rotavirus, and hepatitis E virus in meat cuts marketed in Uruguaiana, Rio Grande do Sul, Brazil

et al. 2022

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“…All of these need to be monitored closely. Several studies in various parts of the world seem to agree on the fact that microbial contaminants are the results of treated and untreated sewage being discharged into shellfsh growing waters, sewage overfow during rainfall periods, and runof from agricultural areas [42,43]. Sewage is loaded with nutrients that, in excessive amounts, could stimulate microbial growth, production of harmful algal blooms, and eutrophication, ultimately afecting the viability of shellfsh mariculture [44].…”

Section: Prevalence Of Faecal Coliforms and Escherichia Coli Inmentioning

confidence: 99%

The Prevalence of Bacteria Commonly Related to the Production of Mussels and Oysters in Saldanha Bay

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et al. 2023

Aquaculture Research

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Bivalve molluscs are a good source of high quality protein and perform important ecological functions. Their ability to bioaccumulate materials in their soft tissues makes them suitable aquatic species for biomonitoring of environmental conditions. The discharge of treated and untreated sewage into the bivalve-growing areas is a concern. The aim of this study was to investigate the prevalence of bacterial microbiota in shellfish farms in Saldanha Bay harbour using pathogens commonly associated with shellfish-related foodborne disease outbreaks. Seawater and mussel samples were collected from five sampling points located in three sampling locations. Oyster samples were collected from the harbour deck immediately after harvesting by the farmers. The most probable number (MPN) method was used to enumerate E. coli and faecal coliforms. Cultural methods were used for the detection of Salmonella and Vibrio spp. The E. coli concentrations for 15 March and 14 July are <0.18 MPN/100 ml at all sampling sites and for 25 August, <0.18 MPN/100 ml for all sampling sites except sampling site SP2 (0.2 MPN/100 ml). Spikes were observed on the total MPN counts in winter. Salmonella and Vibrio spp. were not detected. However, other bacterial species were identified through their phenotypic profile using the VITEK 2 system. Based on the low E. coli-MPN concentrations, the study concluded that the molluscs were safe for human consumption. Further studies need to be conducted on the bacterial species identified.

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“…Bivalve molluscan shellfish like clams and oysters are commonly eaten raw or undercooked are capable to accumulate enteric viruses from their surrounding waters and bioconcentrate them within their digestive systems and, thus, have been reported for some large outbreaks of enteric viruses like norovirus and hepatitis A (Meghnath et al, 2019 ; Richards, 2016 ; Upfold et al, 2021 ). Detection of RVs in sewage water (Hoque et al, 2019a , 2019b ; Thongprachum et al, 2018 ) and in shellfish (Ito et al, 2021 ; Keller et al, 2019 ; Kittigul et al, 2014 ; Quiroz-Santiago et al, 2014 ) has been shown scattered in some studies. However, the seasonal variation of RV detection in shellfish or its association with gastroenteritis epidemics is not yet clear.…”

Section: Introductionmentioning

confidence: 99%

Detection of Rotavirus Strains in Freshwater Clams in Japan

et al. 2022

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Bivalve molluscan shellfish like clams and oysters, etc., are capable to bioaccumulate surrounding contaminants from waters into their digestive systems and posing serious threats of food poisoning. Detection of rotaviruses (RVs) in shellfish is of particular importance because RVs are prone to genome reassortment resulting in the emergence of new RV variants that may compromise vaccine safety. Herein, we have detected the wild-type RVs and Rotarix/RotaTeq vaccine strains in freshwater clams collected on the riverside, Kawasaki city, from July 2019 to January 2020 and correlated the detected genotypes with that of gastroenteritis cases of nearby clinics to understand the transmission of RVs in the environment. The wild-type RVs were detected in 62 (64.6%) out of 96 freshwater clams in every study month: July, September, November, and January that are considered as off-season for RV infections. The most frequent genotypes were G2 (42.9%), G8 (28.6%), G3 (14.3%), G1 (7.1%), and G10 (7.1%), which remained comparable with genotypic distribution found in the clinical samples over the last few years indicating that these RVs may accumulate in clams since a long time. However, G10 genotype was detected in clam but not in clinical samples suggesting the presence of asymptomatic infection or RVs could be carried out from a long distance. Importantly, vaccine strains, RotaTeq (1%) but not Rotarix (0%), were also detected in a clam. Attention must be paid to monitoring the potential transmission of wild-type and vaccine RV strains in the environment to prevent the emergence of new variants generated from genome reassortment with vaccine strains.

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Surveillance of Enteric Viruses and Thermotolerant Coliforms in Surface Water and Bivalves from a Mangrove Estuary in Southeastern Brazil

Cited by 22 publications

References 55 publications

Detection of adenovirus, rotavirus, and hepatitis E virus in meat cuts marketed in Uruguaiana, Rio Grande do Sul, Brazil

Detection of adenovirus, rotavirus, and hepatitis E virus in meat cuts marketed in Uruguaiana, Rio Grande do Sul, Brazil

The Prevalence of Bacteria Commonly Related to the Production of Mussels and Oysters in Saldanha Bay

Detection of Rotavirus Strains in Freshwater Clams in Japan

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