Survival and Recovery of Escherichia coli O157: H7 in Inoculated Bottled Water

Abstract

A methodology used to isolate Escherichia coli O157:H7 from water and survival of this pathogen in inoculated water is described. The methodology used in the isolation of E. coli O157:H7 included the use of selective plating on Sorbitol MacConkey agar (supplemented with potassium tellurite [2.5 mg/liter], cefixime [0.05 mg/liter], and cefsulodin [10 mg/liter], and modified hemorrhagic colitis agar (also supplemented with potassium tellurite [2.5 mg/liter]) and cefsulodin [10 mg/liter]). There were no significant differences (P < 0.05) between the recoveries of E. coli O157:H7 on these two selective media. Direct plating on these selective agars was used to determine the length of time that E. coli O157:H7 was able to grow, remain viable, and be resistant to the selective agents. E. coli O157:H7 survived in inoculated water for up to > 300 days, depending on the type of water. Observation by scanning electron microscopy indicated that E. coli O157:H7 cells attached to, and multiplied on, the container walls.

Loss of O157 O Antigenicity of Verotoxin-Producing Escherichia coli O157:H7 Surviving under Starvation Conditions

“…It is suggested that water is contaminated with E. coli O157:H7 by humans and animals harboring the organism. There are several studies showing survival of E. coli O157:H7 in water for a long time under starvation conditions (12,15,16). For E. coli serotypes other than E. coli O157, expression of genes has been shown to be changed relative to the survival of the organism in water (3,10).…”

Qualidade microbiológica de águas minerais

“…Além disso, a presença de E.coli, indica com maior segurança a possível presença de enteropatógenos. [28], verificaram em seu estudo que E.coli 0157:H7 inoculada em água engarrafada podia sobreviver por mais de 300 dias e que as células deste microrganismo aderiam à superfície interna das garrafas plásticas ou de vidro. JAYASEKARA et al [17], consideram que a aderên-cia à superfície interna das garrafas pode explicar o longo tempo de sobrevivência dos microrganismos na água mineral.…”

Survival of verocytotoxigenicEscherichia coliO157 in soil, water and on surfaces

“…coli 0157 has also been shown to persist for extended periods of time in bottled water (Warburton et al 1998). In these studies commercially available bottled water was inoculated with a mixture of 10 strains of washed E. coli 0157 to a level of 10 3_106 CFUml-l.…”