Survival ofEnterococcus faecalisin infected dentinal tubules after root canal filling with different root canal sealersin vitro

Abstract

Root fillings in vitro with gutta-percha and AH or GS were effective in killing E. faecalis in dentinal tubules. Other endodontic sealers, as well as CH, were less effective.

Influence of the length of remaining root canal filling and post space preparation on the coronal leakage of Enterococcus faecalis

“…According to Pizzo et al, (13), AH Plus sealer, used in this study, presents bacterial inhibition within the first 24 hours, which may have delayed bacterial penetration but was not able to completely prevent coronal leakage of E. faecalis. Therefore, an important role of the endodontic obturation is the formation of a physical barrier to prevent bacteria from reaching the apical region and periapical tissues (16). In Group 1 (root filling remnant of 6 mm), this physical barrier was 3 times larger than that in Group 3 (root filling remnant of 2 mm), which may possibility explain the difference observed between these groups at 20 days concerning the number of specimens with bacterial leakage.…”

“…E. faecalis is the most often retrieved pathogen in asymptomatic endodontic infections because of its capacity to invade dentinal tubules, compete with other microorganisms and resist nutritional privation. It is a facultative anaerobic Gram-positive coccus that may present either isolated, in pairs or in chains (4,16,17,20).…”

“…However, even in these groups the residual obturation was not capable of preventing bacterial leakage, probably due to the presence of gaps at the interface between the filling material and the root canal walls. These interfacial defects may be attributed to variables such as internal anatomy of the root canal system, biomechanical preparation, proprieties of endodontic irrigants, physicochemical proprieties of endodontic materials and root filling techniques (16).…”

“…These properties have been claimed to enhance the dentin/sealer interweaving (19), increase the resistance to removal and/or dislodgment from dentin surface and reduce marginal infiltration (9,16,21). Some factors that may contribute to attainment of different results with respect to bacterial leakage time include study protocol, type of microorganism and its concentration, dental anatomy, amount of remaining tooth structure and sample size (3).…”

Comparative Evaluation of Push-out bond Strength of Three Endodontic Sealers with and without Amoxicillin-An Invitro Study

“…Stevens et al, found that a final rinse with 95% ethanol increased the depth of penetration of a ZnOE based sealers and significantly reduced micro leakage [27]. Tests on dentinal tubule penetration have shown calcium hydroxide based sealers to be less effective in killing bacteria when compared to resin and eugenol based sealers [28,29]. Direct contact tests of sealers and microorganisms concluded calcium hydroxide sealers to be having mild anti-microbial effect over a short duration [30,31].…”